Electrochemical Nucleic Acid-Based Biosensors: Concepts, Terms, and Methodology
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Institute of Analytical Chemistry, Faculty of Chemical and Food Technology, Slovak University of Technology in Bratislava, Radlinského 9, 81237 Bratislava, Slovakia
8Corresponding authors: E-mail: jan.labuda@stuba.sk (J.L.), fojta@ibp.cz (M.F.)
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Department of Chemistry, Faculty of Sciences and Technology, University of Coimbra, 3004-533 Coimbra, Portugal
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Department of Analytical Chemistry, A.M. Butlerov Institute of Chemistry, Kazan State University, Kremlevskaya 18, 420008 Kazan, Russia
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Institute of Biophysics, Academy of Sciences, Královopolská 135, 612 65 Brno, Czech Republic
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Department of Chemistry, University of Florence, Via della Lastruccia 3, 50019 Sesto Fiorentino, Italy
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Faculty of Pharmacy, Ege University, Bornova-Izmir, Turkey 35100
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Department of Nanoengineering, University of San Diego, La Jolla, CA 92093, USA
Project Year: 2006, Project Code: 2006-026-1-500
Cite as: Jan Labuda, Ana Maria Oliveira Brett, Gennady Evtugyn, Miroslav Fojta, Marco Mascini, Mehmet Ozsoz, Ilaria Palchetti, Emil Paleček, Joseph Wang. Electrochemical Nucleic Acid-Based Biosensors: Concepts, Terms, and Methodology, Pure Appl. Chem. 82, 1161 (2010). DOI: https://doi.org/10.1351/PAC-REP-09-08-16
Abstract
An electrochemical nucleic acid (NA)-based biosensor is a biosensor that integrates a nucleic acid as the biological recognition element and an electrode as the electrochemical signal transducer. The present report provides concepts, terms, and methodology related to biorecognition elements, detection principles, type of interactions to be addressed, and construction and performance of electrochemical NA biosensors, including their critical evaluation, which should be valuable for a wide audience, from academic, biomedical, environmental, and food-testing, drug-developing, etc. laboratories to sensor producers.
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