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Multiphoton Microscopy and Fluorescence Lifetime Imaging

Applications in Biology and Medicine

Ed. by König, Karsten

With contrib. by Baldeweck, Thérèse / Balu, Mihaela / Batista, Ana / Vecker, Wolfgang / Belousov, Vsevolod V / Breinig, Hans Georg / Breymayer, Jasmin / Brizion, Sébastien / Castello, Marco / Chandra, Dhyan / Choi, Heejin / Decencière, Etienne / Diaspro, Alberto / Donitzky, Christoph / Dudenkova, Varvara V. / Druzhkova, Irina N. / Harris, Ronald M. / Hoffman, Robert M. / Holmes, Amy / Huck, Volker / Joshi, Suchitra / Kaiser, Wolfgang / Kalinina, Sviatlana / Kantelhardt, Sven R. / Kapur, Jaideep / Kelly, Kristen M. / König, Karten / Koudoro, Serge / Krueger, Arnd / Lanzano, Luca / Liang, Xiaowen / Liu, Xin / Lukina, Maria M. / Mascaro, Anna Letizia Allegra / Melia, Meghan J.O. / Mess, Christian / Mishina, Natalie M. / Pavone, Francesco S. / Periasamy, Ammasi / Rehman Alam, Shagufta / Roberts, Michael S. / Pena, Ana-Maria / Rowlands, Christoph / Rück, Angelika / Sacconi, Leonardo / Scipioni, Lorenzo / Sergeeva, Tatiana F. / Shcheslavskiy, Vladislav I. / Shirmanova, Marina V. / Silvestri, Ludovico / So, Peter T.C. / Studier, Hauke / Svindrych, Zdenek / Tancrede-Bohin, Emmanuelle / Thorling, Camilla / Tromberg, Bruce j. / Uchugonova, Aisada / Vicidomini, Giuseppe / Victorin, Steeve / Wallrabe, Horst / Wang, Haolu / Weinigel, Martin / Yew, Elijah / Zachary, Christopher B. / Zagaynova, Elena V.

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January 2018
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1 Brief history of fluorescence lifetime imaging

König, Karsten


This review gives an overview of the history of fluorescence lifetime imaging (FLIM) in life sciences. FLIM microscopy based on an ultrafast laser scanning microscope and time-correlated single photon counting (TCSPC) was introduced in Jena/ Germany in 1988/89. FLIM images of porphyrin-labeled live cells and live mice were taken with an unique ZEISS confocal picosecond laser microscope. Five years later, the first in vivo FLIM on human volunteers started with time-gated cameras to detect dental caries based on one-photon wide-field pulsed laser excitation of autofluorescent bacteria. Another five years later, two-photon FLIM of autofluorescent skin was performed on a volunteer with a lab microscope in the frequency domain. The first clinical non-invasive optical, two-photon 3DFLIMbiopsieswere obtained fifteen years ago in patientswith dermatological disorders using a certified clinical multiphoton tomograph based on a tunable femtosecond titanium:sapphire laser and TCSPC. A current major FLIM application in cell biology is the study of protein-protein interactions in transfected cells by FLIM-FRETmicroscopy. Clinical FLIMapplications are still on a research level and include preliminary studies on (i) one-photon FLIM autofluorescence microscopy of patients with ocular diseases using picosecond laser diodes, (ii) time-gated imaging in brain surgery using a nanosecond nitrogen laser, and (iii) two-photon clinical FLIM tomography of patients with skin cancer and brain tumors with near-infrared femtosecond lasers and TCSPC.

Citation Information

Karsten König (2018). 1 Brief history of fluorescence lifetime imaging. In Karsten König (Editor), Multiphoton Microscopy and Fluorescence Lifetime Imaging: Applications in Biology and Medicine (pp. 3–16). Berlin, Boston: De Gruyter. https://doi.org/10.1515/9783110429985-003

Book DOI: https://doi.org/10.1515/9783110429985

Online ISBN: 9783110429985

© 2018 Walter de Gruyter GmbH, Berlin/Munich/Boston. BY 3.0 This work is licensed under the Creative Commons Attribution 3.0 Public License.

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