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Multiphoton Microscopy and Fluorescence Lifetime Imaging

Applications in Biology and Medicine

Ed. by König, Karsten

With contrib. by Baldeweck, Thérèse / Balu, Mihaela / Batista, Ana / Vecker, Wolfgang / Belousov, Vsevolod V / Breinig, Hans Georg / Breymayer, Jasmin / Brizion, Sébastien / Castello, Marco / Chandra, Dhyan / Choi, Heejin / Decencière, Etienne / Diaspro, Alberto / Donitzky, Christoph / Dudenkova, Varvara V. / Druzhkova, Irina N. / Harris, Ronald M. / Hoffman, Robert M. / Holmes, Amy / Huck, Volker / Joshi, Suchitra / Kaiser, Wolfgang / Kalinina, Sviatlana / Kantelhardt, Sven R. / Kapur, Jaideep / Kelly, Kristen M. / König, Karten / Koudoro, Serge / Krueger, Arnd / Lanzano, Luca / Liang, Xiaowen / Liu, Xin / Lukina, Maria M. / Mascaro, Anna Letizia Allegra / Melia, Meghan J.O. / Mess, Christian / Mishina, Natalie M. / Pavone, Francesco S. / Periasamy, Ammasi / Rehman Alam, Shagufta / Roberts, Michael S. / Pena, Ana-Maria / Rowlands, Christoph / Rück, Angelika / Sacconi, Leonardo / Scipioni, Lorenzo / Sergeeva, Tatiana F. / Shcheslavskiy, Vladislav I. / Shirmanova, Marina V. / Silvestri, Ludovico / So, Peter T.C. / Studier, Hauke / Svindrych, Zdenek / Tancrede-Bohin, Emmanuelle / Thorling, Camilla / Tromberg, Bruce j. / Uchugonova, Aisada / Vicidomini, Giuseppe / Victorin, Steeve / Wallrabe, Horst / Wang, Haolu / Weinigel, Martin / Yew, Elijah / Zachary, Christopher B. / Zagaynova, Elena V.

Open Access
eBook (PDF)
Publication Date:
January 2018
Copyright year:
2018
ISBN
978-3-11-042998-5
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6 Principles and applications of temporal-focusing wide-field two-photon microscopy

So, Peter T. C. / Choi, Heejin / Yew, Elijah / Rowlands, Christopher

Abstract

Temporal focusing allows for rapid optically sectioned two-photon widefield microscopy. Depth sectioning is provided in a wide-field manner, without spatial focusing, by controlling the temporalwidth of femtosecond laser pulses near the focal plane. This spatial control of the temporal pulse width is achieved by diffracting the light off a grating resulting in spectral component separation and temporal broadening; these spectral components are only recombined at the focal plane to reproduce short, femtosecond pulses. Applications include (i) high speed functional imaging in the brain, (ii) fast FLIM and PLIM, (iii) cell-selective optogenetic excitation, and (iv) temporal focusing photodynamic therapy that may allow selective killing of cancer cells.

Citation Information

Peter T. C. So, Heejin Choi, Elijah Yew, Christopher Rowlands (2018). 6 Principles and applications of temporal-focusing wide-field two-photon microscopy. In Karsten König (Editor), Multiphoton Microscopy and Fluorescence Lifetime Imaging: Applications in Biology and Medicine (pp. 103–140). Berlin, Boston: De Gruyter. https://doi.org/10.1515/9783110429985-008

Book DOI: https://doi.org/10.1515/9783110429985

Online ISBN: 9783110429985

© 2018 Walter de Gruyter GmbH, Berlin/Munich/Boston. BY 3.0 This work is licensed under the Creative Commons Attribution 3.0 Public License.

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