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Stem Cells - From Drug to Drug Discovery

Ed. by Haider, Khawaja Husnain

With contrib. by Tong, Cao / Gopu, Sriram / Saleem, Asmat / Khan, Irfan / Naeem, Nadia / Ali, Anwar / Sheikh, Riazuddin / Tarar, Moazzam Nazeer / Mehmood, Azra / Latief, Noreen / Alsharida, Mansour / Abdalla, Elmuataz Elmansi / Afroze, Salman / Zhu, Kai / Guo, Changfa / Ziaee, Seyyed Mohyeddin / Tabeshmehr, Parisa / Lei, Ye

eBook (PDF)
Publication Date:
March 2017
Copyright year:
2017
ISBN
978-3-11-049376-4
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1. Human pluripotent stem-cell-derived vascular cells: in vitro model for angiogenesis and drug discovery

Gopu, Sriram / Tong, Cao

Abstract

Angiogenesis is the process of formation of new blood vessels in development, health and diseased states. It is an intricate process involving a multitude of overlapping phases that encompass cell-cell and cell-matrix interactions between the vascular cells (in particular, endothelial cells and vascular smooth muscle cells/ pericytes) and the surrounding extracellular matrix. Assays to investigate angiogenesis are used in the screening of novel proangiogenic and antiangiogenic drug candidates that have potential in the treatment of ischemic diseases, cancers, a range of vascular disorders and for therapeutic vascularization in regenerative medicine. Most assays rely on in vitro models of angiogenesis using monolayer cultures of primary and immortalized ECs and in vitro animal models. Pluripotent stem cells (PSCs) like human embryonic stem cells (ESCs) due to their ability to self-renew, differentiate to any cell type in the body, and recapitulate the embryological, physiological, and pathological states of angiogenesis, provide a promising in vitro cellular platform for development of novel proangiogenic and antiangiogenic drug candidates. This chapter highlights the benefits of using human PSC-derived vascular cells for drug discovery, the methods developed in our laboratory for differentiation of human ESCs to vascular cells and various co-culture models for drug screening studies. Further, we will discuss some of the obstacles and challenges that need to be addressed for stem-cell-based approaches to be used as an effective tool in the pursuit to develop clinically successful drug compounds.

Citation Information

Sriram Gopu, Cao Tong (2017). 1. Human pluripotent stem-cell-derived vascular cells: in vitro model for angiogenesis and drug discovery. In Khawaja Husnain Haider (Editor), Stem Cells - From Drug to Drug Discovery (pp. 1–34). Berlin, Boston: De Gruyter. https://doi.org/10.1515/9783110493764-001

Book DOI: https://doi.org/10.1515/9783110493764

Online ISBN: 9783110493764

© 2017 Walter de Gruyter GmbH, Berlin/Munich/BostonGet Permission

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