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Stem Cells - From Drug to Drug Discovery

Ed. by Haider, Khawaja Husnain

With contrib. by Tong, Cao / Gopu, Sriram / Saleem, Asmat / Khan, Irfan / Naeem, Nadia / Ali, Anwar / Sheikh, Riazuddin / Tarar, Moazzam Nazeer / Mehmood, Azra / Latief, Noreen / Alsharida, Mansour / Abdalla, Elmuataz Elmansi / Afroze, Salman / Zhu, Kai / Guo, Changfa / Ziaee, Seyyed Mohyeddin / Tabeshmehr, Parisa / Lei, Ye

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March 2017
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6. Nanoparticle-based genetic engineering of mesenchymal stem cells

Guo, Changfa / Zhu, Kai / Haider, Khawaja Husnain


Given their ease of availability, in vitro expansion without altering their multilineage differentiation potential and paracrine behavior and, combined with the data from in vitro characterization studies, preclinical and clinical studies in human patients, mesenchymal stem cells (MSCs) are being viewed as choice cells for cell-based therapies. Although the results have been reassuring in terms of their ability to cross lineage restriction and adopt different functionally competent phenotypes, MSCs are also being conjoined with gene delivery to augment their therapeutic benefits. In this context, a safe, efficient, biocompatible and regulatable gene transfer vehicle is warranted that might effectively transfer genes of interest into MSCs. In comparison with the conventional gene transfer vectors, which suffer from issues vis-a-vis their safety, poor transfection efficiency, toxicity and immunogenicity, nanoparticle-based gene delivery vehicles afford safe, efficient and cost-effective alternatives. Numerous nano-vehicles with particle sizes of less than 100 nm have been designed with superior transgene delivery efficiency and biocompatibility. Likewise, with beacon-like and missile-like modifications, nanoparticles are being used for tracking the fate of transplanted cells in real-time. This chapter provides an overview of the application of nano-vehicles for genetic engineering of cells with a focus on MSCs. Key issues and future directions in nanoparticle-based gene transfer have also critically addressed.

Citation Information

Changfa Guo, Kai Zhu, Khawaja Husnain Haider (2017). 6. Nanoparticle-based genetic engineering of mesenchymal stem cells. In Khawaja Husnain Haider (Editor), Stem Cells - From Drug to Drug Discovery (pp. 129–147). Berlin, Boston: De Gruyter. https://doi.org/10.1515/9783110493764-006

Book DOI: https://doi.org/10.1515/9783110493764

Online ISBN: 9783110493764

© 2017 Walter de Gruyter GmbH, Berlin/Munich/BostonGet Permission

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