Characterization of Biological Membranes
Structure and Dynamics
Ed. by Nieh, Mu-Ping / Heberle, Frederick A. / Katsaras, John
With contrib. by Bowerman, Charles / Bozelli Junior, José Carlos / Brown, Michael F. / Butler, Paul D. / Chan, Chun / Cheng, Xiaolin / Cherniavskyi, Yevhen / Cheung, Eugene / Chini, Corryn E. / Craig, Andrew F. / Dabney-Smith, Carole / Davis, James H. / Dimova, Rumiana / DiPasquale, Mitchell / Eells, Rebecca / Epand, Richard M. / Fisher, Alessandro / Frank, Kilian / Gorman, Brittney L. / Harroun, Thad A. / Heinrich, Frank / Hoogerheide, David P. / Kelly, Elizabeth G. / Kienzle, Paul A. / Kiessling, Volker / Komorowski, Karlo / Konkolewicz, Dominik / Kraft, Mary / Kučerka, Norbert / Li, Ying / London, Erwin / Lorigan, Gary A. / Lösche, Mathias / Majkrzak, Charles F. / Marquardt, Drew / Nagao, Michihiro / Nguyen, Michael H.L. / Nickels, Jonathan / Poloni, Laura / Sahu, Indra D. / Salditt, Tim / Schick, Michael / Schmidt, Miranda / Shen, Zhingiang / Steinkühler, Jan / Tamm, Lukas K. / Tieleman, Peter / Uhríková, Daniela / Won, Amy / Xia, Yan / Ye, Huilin / Yeager, Ashley N. / Yip, Christopher M.
Series:De Gruyter STEM
- eBook (PDF)
- Publication Date:
- July 2019
- Copyright year:
5. Spontaneous lipid transfer rate constants
Xia, Yan / Nieh, Mu-Ping
This chapter will introduce three different experimental approaches to measure spontaneous lipid transfer rate constants between lipid bilayers, including timeresolved small angle neutron scattering (TR-SANS), differential scanning calorimetry (DSC), and fluorescence correlation spectroscopy (FCS). The basic principle of TR-SANS and DSC is applicable if the neutron scattering length density (NSLD) (in the case of TRSANS) or melting transition temperature (in the case of DSC) of the lipid in study is sufficiently different fromthat of its deuterated counterpart.Withminimal disturbance of the chemical properties of the systemusing the isotope molecules, these two approaches presumably yield more accurate lipid transfer rate constant than the outcome obtained from FCS, which requires the use of fluorophores having a similar chemical structure of the lipid in study. Examples of measuring lipid transfer rate constants will be demonstrated in two well-defined nanoparticles (NPs), discoidal bicelles and unilamellar vesicles (ULVs). At the end of this chapter, we summarize the pros and cons of each method in order to provide the researchers with the principles of selecting the appropriate method for the systems of their interest.
Yan Xia, Mu-Ping Nieh (2019). 5. Spontaneous lipid transfer rate constants. In Mu-Ping Nieh, Frederick A. Heberle, John Katsaras (Eds.), Characterization of Biological Membranes: Structure and Dynamics (pp. 177–194). Berlin, Boston: De Gruyter. https://doi.org/10.1515/9783110544657-005
Book DOI: https://doi.org/10.1515/9783110544657
Online ISBN: 9783110544657© 2019 Walter de Gruyter GmbH, Berlin/Munich/Boston