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Characterization of Biological Membranes

Structure and Dynamics

Ed. by Nieh, Mu-Ping / Heberle, Frederick A. / Katsaras, John

With contrib. by Bowerman, Charles / Bozelli Junior, José Carlos / Brown, Michael F. / Butler, Paul D. / Chan, Chun / Cheng, Xiaolin / Cherniavskyi, Yevhen / Cheung, Eugene / Chini, Corryn E. / Craig, Andrew F. / Dabney-Smith, Carole / Davis, James H. / Dimova, Rumiana / DiPasquale, Mitchell / Eells, Rebecca / Epand, Richard M. / Fisher, Alessandro / Frank, Kilian / Gorman, Brittney L. / Harroun, Thad A. / Heinrich, Frank / Hoogerheide, David P. / Kelly, Elizabeth G. / Kienzle, Paul A. / Kiessling, Volker / Komorowski, Karlo / Konkolewicz, Dominik / Kraft, Mary / Kučerka, Norbert / Li, Ying / London, Erwin / Lorigan, Gary A. / Lösche, Mathias / Majkrzak, Charles F. / Marquardt, Drew / Nagao, Michihiro / Nguyen, Michael H.L. / Nickels, Jonathan / Poloni, Laura / Sahu, Indra D. / Salditt, Tim / Schick, Michael / Schmidt, Miranda / Shen, Zhingiang / Steinkühler, Jan / Tamm, Lukas K. / Tieleman, Peter / Uhríková, Daniela / Won, Amy / Xia, Yan / Ye, Huilin / Yeager, Ashley N. / Yip, Christopher M.

Series:De Gruyter STEM

eBook (PDF)
Publication Date:
July 2019
Copyright year:
2019
ISBN
978-3-11-054465-7
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6. Fundamentals of Nuclear Magnetic Resonance spectroscopy (NMR) and its applications

Cheung, Eugene / Cash, Darian

Abstract

Nuclear magnetic resonance (NMR) spectroscopy is an eminent technique for the characterization of structure and dynamics of molecules in solution and in the solid state. With applications in a majority of the physical and biological sciences, ranging from chemistry and materials science to mineralogy and molecular biology, the frontiers of NMR spectroscopic analysis seem limited only by the creativity of its users. NMR spectroscopy provides exquisite detail about the nuclear environment of any molecular system with little, if any, perturbation of the system during analysis. In the context of analyzing bilayers, biomimetics, or biological molecules, variants of 1H, 13C, 15N, and 31P NMR experiments are extending the reach of NMR spectroscopy for tackling key physical and chemical aspects of biologic drugs and drug-delivery systems. Pseudo-2D NMR experiments such as pulsed gradient spin echo NMR spectroscopy are exceptionally powerful in studying translational motion directly in biological fluids and under simulated physiological conditions. The NOESY experiment gives correlation between two nuclei that are within several angstroms from each other and is unique in its ability to elucidate membrane-bound entities. New NMR pulse sequences are invented and technological breakthroughs are constantly adopted as the ever expanding arsenal of NMR experiments continues to inspire generations of scientists. For characterization of complex structures such as biomembranes and nanoparticles, NMR spectroscopy is perhaps the only analytical technique that can elucidate structure and dynamics in situ and without dilution.

Citation Information

Eugene Cheung, Darian Cash (2019). 6. Fundamentals of Nuclear Magnetic Resonance spectroscopy (NMR) and its applications. In Mu-Ping Nieh, Frederick A. Heberle, John Katsaras (Eds.), Characterization of Biological Membranes: Structure and Dynamics (pp. 195–230). Berlin, Boston: De Gruyter. https://doi.org/10.1515/9783110544657-006

Book DOI: https://doi.org/10.1515/9783110544657

Online ISBN: 9783110544657

© 2019 Walter de Gruyter GmbH, Berlin/Munich/BostonGet Permission

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