Characterization of Biological Membranes
Structure and Dynamics
Ed. by Nieh, Mu-Ping / Heberle, Frederick A. / Katsaras, John
With contrib. by Bowerman, Charles / Bozelli Junior, José Carlos / Brown, Michael F. / Butler, Paul D. / Chan, Chun / Cheng, Xiaolin / Cherniavskyi, Yevhen / Cheung, Eugene / Chini, Corryn E. / Craig, Andrew F. / Dabney-Smith, Carole / Davis, James H. / Dimova, Rumiana / DiPasquale, Mitchell / Eells, Rebecca / Epand, Richard M. / Fisher, Alessandro / Frank, Kilian / Gorman, Brittney L. / Harroun, Thad A. / Heinrich, Frank / Hoogerheide, David P. / Kelly, Elizabeth G. / Kienzle, Paul A. / Kiessling, Volker / Komorowski, Karlo / Konkolewicz, Dominik / Kraft, Mary / Kučerka, Norbert / Li, Ying / London, Erwin / Lorigan, Gary A. / Lösche, Mathias / Majkrzak, Charles F. / Marquardt, Drew / Nagao, Michihiro / Nguyen, Michael H.L. / Nickels, Jonathan / Poloni, Laura / Sahu, Indra D. / Salditt, Tim / Schick, Michael / Schmidt, Miranda / Shen, Zhingiang / Steinkühler, Jan / Tamm, Lukas K. / Tieleman, Peter / Uhríková, Daniela / Won, Amy / Xia, Yan / Ye, Huilin / Yeager, Ashley N. / Yip, Christopher M.
Series:De Gruyter STEM
- eBook (PDF)
- Publication Date:
- July 2019
- Copyright year:
15. Application and characterization of asymmetric-supported membranes
Kiessling, Volker / Tamm, Lukas K.
The plasma membrane of cells is a heterogeneous compartment that consists of a variety of different lipid and protein species. Its structure and dynamics are determined by complex lipid-lipid, protein-protein, and lipid-protein interactions within the membrane and with its environment. Studies of model membranes have played crucial roles and contributed essential new knowledge to our current understanding of the structure and function of cell membranes. Recently, several groups have developed new model membrane systems in order to study consequences of the compositional asymmetry between the two leaflets of the plasma membrane. Our group has focused on the development, characterization, and application of asymmetric-supported planar bilayers. In this chapter, we first summarize methods of preparation and properties of asymmetric supported planar bilayers. We then highlight how we have utilized these membranes to address relevant biological questions in the fields of trans-bilayer lipid domain coupling, soluble N-ethylmaleimide- sensitive factor attachment proteins receptor (SNARE)-mediated membrane fusion, and viral entry into cells.
Volker Kiessling, Lukas K. Tamm (2019). 15. Application and characterization of asymmetric-supported membranes. In Mu-Ping Nieh, Frederick A. Heberle, John Katsaras (Eds.), Characterization of Biological Membranes: Structure and Dynamics (pp. 465–476). Berlin, Boston: De Gruyter. https://doi.org/10.1515/9783110544657-015
Book DOI: https://doi.org/10.1515/9783110544657
Online ISBN: 9783110544657© 2019 Walter de Gruyter GmbH, Berlin/Munich/Boston