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Stem Cells: From Myth to Reality and Evolving

Ed. by Haider, Khawaja Husnain

eBook (PDF)
Publication Date:
September 2019
Copyright year:
2019
ISBN
978-3-11-064243-8
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5. Influence of selected cryoprotectants on regeneration of cryopreserved cells properties

Jelínková, Šárka / Kratochvílová, Irena / Pešl, Martin / Skládal, Petr / Rotrekl, Vladimír / Přibyl, Jan

Abstract

The cryopreservation of cells is a fundamental task for a wide range of applications, from basic storage of cells to fertilization. Cells must be protected against damage during freezing by application of specific cryoprotectants and freezing/melting protocols. This chapter presents a brief overview of the predominant cryopreservation methods and most common cryoprotectants. Furthermore, it explores the specifics of cryopreservation of stem cells with various types and levels of multipotency. Special attention is dedicated to the effect of cryopreservation on human pluripotent stem cells, including the effect of different cryoprotectants used to prepare fibroblast feeder layer for the cultivation of pluripotent cells. Finally, the chapter concludes with a review of selected methods that have been successfully used to probe the postthaw state of cryopreserved cells through their viability, inner structure, and mechanical properties.

Citation Information

Šárka Jelínková, Irena Kratochvílová, Martin Pešl, Petr Skládal, Vladimír Rotrekl, Jan Přibyl (2019). 5. Influence of selected cryoprotectants on regeneration of cryopreserved cells properties. In Khawaja Husnain Haider (Editor), Stem Cells: From Myth to Reality and Evolving (pp. 102–126). Berlin, Boston: De Gruyter. https://doi.org/10.1515/9783110642438-005

Book DOI: https://doi.org/10.1515/9783110642438

Online ISBN: 9783110642438

© 2019 Walter de Gruyter GmbH, Berlin/Munich/BostonGet Permission

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