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Acta Biologica Cracoviensia s. Botanica

The Journal of Polish Academy of Sciences


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1898-0295
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Using Morphological Characters and Simple Sequence Repeat (SSR) Markers to Characterize Tunisian Fig (Ficus Carica L.) Cultivars

Olfa Saddoud1 / Ghada Baraket1 / Khaled Chatti1 / Mokhtar Trifi1 / Mohamed Marrakchi1 / Messoud Mars1 / Amel Salhi-Hannachi1

Laboratory of Molecular Genetics, Immunology and Biotechnology, Tunis Campus University, 2092 El Manar Tunis, Tunisia1

Agronomic High Institute of Chott Mariem, 4042 Sousse, Tunisia2

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Citation Information: Acta Biologica Cracoviensia Series Botanica. Volume 53, Issue 2, Pages 7–14, ISSN (Online) 1898-0295, ISSN (Print) 0001-5296, DOI: 10.2478/v10182-011-0019-y, January 2012

Publication History

Published Online:
2012-01-02

Using Morphological Characters and Simple Sequence Repeat (SSR) Markers to Characterize Tunisian Fig (Ficus Carica L.) Cultivars

We used simple sequence repeat markers and 25 morphological characters to characterize 18 Tunisian fig (Ficus carica L.) cultivars. Morphological traits suggested a high level of variation in the germplasm. Principal component analysis (PCA) differentiated the studied cultivars. In the derived dendrogram the cultivars clustered independently of their geographical origin and sex of trees. Simple sequence repeat (SSR) markers were used to compare genetic polymorphism with the observed phenotypic variation. Using six microsatellite primers, 39 alleles and 59 genotypes were identified. The high values of polymorphism information content (PIC), ranging from 0.67 to 0.85, confirmed the effectiveness of microsatellite analysis for determining molecular polymorphism and characterizing the studied cultivars. Multilocus genotyping unambiguously distinguished all the cultivars. The ability of each type of feature to differentiate cultivars of this crop is discussed.

Keywords: Cultivars; Ficus carica L.; morphological characters; microsatellites; Tunisia; polymorphism

  • Baraket G, Chatti K, Saddoud O, Mars M, Marrakchi M, Trifi M, and Salhi-Hannachi A. 2009. Genetic analysis of Tunisian fig (Ficus carica L.) cultivars using amplified fragment length polymorphism (AFLP) markers. Scientia Horticulturae 120: 487-492. [Web of Science]

  • Belkhir K. 2001. Genetix version 4.0 Conception genome & populations programmation. Belkhir Biosoft, Laboratoire Génome, populations, interactions CNRS UPR 9060, Univ. Montpellier II, Montpellier, France.

  • Cavalli-Sforza LL, and Edwards AWF. 1967 Phylogenetic analysis: models and estimation procedures. American Journal of Human Genetics 19: 233-257.

  • Chatti K, Saddoud O, Salhi-Hannachi A, Mars M, Marrakchi M, and Trifi M. 2007. Analysis of genetic diversity and relationships in a Tunisian fig (Ficus carica) germplasm collection by Random Amplified Microsatellite Polymorphisms. Journal of Integrative Plant Biology 49(3): 386-391. [Web of Science] [Crossref]

  • Chatti K, Salhi-Hannachi A, Mars M, Marrakchi M, and Trifi M. 2004a. Analyse de la diversité génétique de cultivars tunisiens de figuier (Ficus carica L.) à l'aide de caractères morphologiques. Fruits 59: 49-61. [Crossref]

  • Chatti K, Salhi-Hannachi A, Mars M, Marrakchi M, and Trifi M. 2004b. Genetic diversity and phylogenetic relationships in Tunisian fig (Ficus carica L.) cultivars mediated by RAPD. Bioliogia Tunisie 1: 1-4.

  • Cregan P.B, Jarvik I, Bush AL, Shoemaker RC, Lark KG, Kehler AL, Kaya N, Vantoai TT, Lohnes DG, Chung J, and Specht JE. 1999. An integrated genetic linkage map soybean genome. Crop Science 39: 1464-1490.

  • Dean RE, Dahlberg JA, Hopkins MS, Mitchell CV, and Kresovich S. 1999. Genetic redundancy and diversity among ‘orange’ accessions in the US national sorghum collection assessed with simple sequence repeat (SSR) markers. Crop Science 39: 1215-1221.

  • Dellaporta SL, Wood J, and Hicks JB. 1983. A plant DNA preparation, Version II. Plant Molecular Biology Reporter 4: 19-21. [Crossref]

  • Felsenstein J. 1995. PHYLIP (Phylogeny Interference Package) version 3,5 c. Department of Genetics, University of Washington, Seattle, Washington

  • Goulão L, Cabrita L, Oliverita CM, and Leitao JM. 2001. Comparing RAPD and AFLP analysis in discrimination and estimation of genetic similarities among apple (Malus domestica Borkh.) cultivars. Euphytica 119: 259-270.

  • Gupta PK, and Varshney K. 2000. The development and use of microsatellite markers for genetic analysis and plant breeding with the emphasis on bread wheat. Euphytica 113: 163-185.

  • Ipgri and Ciheam. 2003. Descriptors for fig (Ficus carica L.). IPGRI, CIHEAM.

  • Khadari B, Hochu I, Santoni S, and Kjellberg F. 2001. Identification and characterization of microsatellite loci in the common fig (Ficus carica L.) and representative species of the genus Ficus. Molecular Ecology Notes 1: 191-193.

  • Kislev EM, Hartmann A, and Bar-Yosef O. 2006. Early domesticated Fig in the Jordan Valley. Science 312 (5778): 1372-1374.

  • Kjellberg F, Gouyon PH, Ibrahim M, Raymond M, and Valdeyron G. 1987. The stability of the symbiosis between dioecious figs and their pollinators: a study of Ficus carica L. and Blastophaga psenes L. Evolution 41: 693-704

  • Machado CA, Jousselin E, Kjellberg F, Compton SG, and Herre EA. 2001. Phylogenetic relationships, historical biogeography and character evolution of fig pollinating wasps. Proceedings of the Royal Society London Biological Science 268: 7-10.

  • Mantel N. 1967. The detection of disease clustering and a generalized Regression approach. Cancer Research 27: 209-220.

  • Mars M, Chelbi T, and Marrakchi M. 1998 Multivariate analysis of fig (Ficus carica L.) germplasm in southern Tunisia. Acta Horticulturae 480: 75-81.

  • Nei M. 1978 Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89: 583-590.

  • Page RDM. 1998. TREEVIEW: an application to display phylogenetic trees on personal computers. Computer Applications in the Biosciences 12: 357-358.

  • Powell W, Morgante M, Andre C, Hanafey M, Vogel J, Tingey S, and Rafalski A. 1996. The comparison of RFLP, RAPD, AFLP, and SSR (microsatellites) markers for germplasm analysis. Molecular Breeding 2: 225-238. [Crossref]

  • Rhouma A. 1996. Les ressources phytogénétiques oasiennes: le figuier (Ficus carica L.). Actes des 3èmes journées nationales sur les acquis de la recherche agronomique, vétérinaire et halieutique, Nabeul, Tunisie.

  • Saddoud O, Salhi-Hannachi A, Chatti K, Rhouma A, Mars M, Marrakchi M, and Trifi M. 2005. Tunisian Fig (Ficus carica L.) genetic diversity and cultivars identification mediated by microsatellites markers. Fruits 60 (2): 143-153. [Crossref]

  • Saddoud O, Chatti K, Salhi-Hannachi A, Mars M, Rhouma A, Marrakchi M, and TRIFI M. 2007. Genetic diversity of Tunisian figs (Ficus carica L.) as revealed by nuclear microsatellites. Hereditas 144: 149-157. [Web of Science]

  • Salhi-Hannachi A, Chatti K, Saddoud O, Messaoud M, Rhouma A, Marrakchi M, and Trifi M. 2006. Genetic diversity of different Tunisian fig (Ficus carica L.) collections revealed by RAPD fingerprints. Hereditas 143: 15-22.

  • Sambrook J, Frithsch EF, and Maniatis T. 1989. Molecular Cloning: A Laboratory Manual, 2nd ed., Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, USA.

  • SAS. 1990. SAS user's guide: SAS/STAT, SAS BASIC. Version 6.07, fourth edition.

  • Simioniuc D, Uptmoor R, Friedt W, and Ordon F. 2002. Genetic diversity and relationships among pea cultivars (Pisum sativum L.) revealed by RAPDs and AFLPs. Plant Breeding 121: 429-435.

  • Storey WB, Enderud JE, Saleeb WF, and Nauer EM. 1977. The Fig. Jurupa Mountains Cultural Center, Riverside, California.

  • Tanaka K, Tsumura Y, and Nakamura T. 1999. Development and polymorphism of microsatellite markers for Fagus crenata and the closely related species F. japonica.Theoretical and Applied Genetics 99: 11-15.

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[1]
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ISRN Agronomy, 2012, Volume 2012, Page 1

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