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Acta Biologica Cracoviensia s. Botanica

The Journal of Polish Academy of Sciences

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Using Morphological Characters and Simple Sequence Repeat (SSR) Markers to Characterize Tunisian Fig (Ficus Carica L.) Cultivars

Olfa Saddoud1 / Ghada Baraket1 / Khaled Chatti1 / Mokhtar Trifi1 / Mohamed Marrakchi1 / Messoud Mars1 / Amel Salhi-Hannachi1

Laboratory of Molecular Genetics, Immunology and Biotechnology, Tunis Campus University, 2092 El Manar Tunis, Tunisia1

Agronomic High Institute of Chott Mariem, 4042 Sousse, Tunisia2

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Citation Information: Acta Biologica Cracoviensia Series Botanica. Volume 53, Issue 2, Pages 7–14, ISSN (Online) 1898-0295, ISSN (Print) 0001-5296, DOI: 10.2478/v10182-011-0019-y, January 2012

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Using Morphological Characters and Simple Sequence Repeat (SSR) Markers to Characterize Tunisian Fig (Ficus Carica L.) Cultivars

We used simple sequence repeat markers and 25 morphological characters to characterize 18 Tunisian fig (Ficus carica L.) cultivars. Morphological traits suggested a high level of variation in the germplasm. Principal component analysis (PCA) differentiated the studied cultivars. In the derived dendrogram the cultivars clustered independently of their geographical origin and sex of trees. Simple sequence repeat (SSR) markers were used to compare genetic polymorphism with the observed phenotypic variation. Using six microsatellite primers, 39 alleles and 59 genotypes were identified. The high values of polymorphism information content (PIC), ranging from 0.67 to 0.85, confirmed the effectiveness of microsatellite analysis for determining molecular polymorphism and characterizing the studied cultivars. Multilocus genotyping unambiguously distinguished all the cultivars. The ability of each type of feature to differentiate cultivars of this crop is discussed.

Keywords: Cultivars; Ficus carica L.; morphological characters; microsatellites; Tunisia; polymorphism

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