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Medical Journal of Cell Biology

The Journal of Foundation for Cell Biology and Molecular Biology

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Amino acids metabolism and degradation is regulated during porcine oviductal epithelial cells (OECs) primary culture in vitro – a signaling pathways activation approach

Wiesława Kranc / Maurycy Jankowski / Joanna Budna / Piotr Celichowski / Ronza Khozmi / Artur Bryja / Sylwia Borys / Marta Dyszkiewicz-Konwińska
  • Department of Anatomy, Poznan University of Medical Sciences, Poznan, Poland
  • Department of Biomaterials and Experimental Dentistry, Poznan University of Medical Sciences, Poznan, Poland
  • Other articles by this author:
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/ Michal Jeseta
  • Department of Obstetrics and Gynecology, University Hospital and Masaryk University, Obilni trh 11, 602 00 Brno, Czech Republic
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/ Magdalena Magas
  • Department of Anatomy, Poznan University of Medical Sciences, Poznan, Poland
  • Veterinary Center, Nicolaus Copernicus University in Toruń, Torun, Poland
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/ Dorota Bukowska / Paweł Antosik / Klaus P. Brüssow / Małgorzata Bruska / Michał Nowicki / Maciej Zabel
  • Department of Histology and Embryology, Poznan University of Medical Sciences, Poznan, Poland
  • Department of Histology and Embryology, Wroclaw Medical University, Wroclaw, Poland
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/ Bartosz Kempisty
  • Corresponding author
  • Department of Anatomy, Poznan University of Medical Sciences, Poznan, Poland
  • Department of Histology and Embryology, Poznan University of Medical Sciences, Poznan, Poland
  • Department of Obstetrics and Gynecology, University Hospital and Masaryk University, Obilni trh 11, 602 00 Brno, Czech Republic
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Published Online: 2018-01-31 | DOI: https://doi.org/10.2478/acb-2018-0004

Abstract

The ovary is part of the reproductive system, possessing very important functions in the reproduction process (ovum and embryo transfer, providing a suitable environment for sperm capacitation, etc.). There are two types of cells in the fallopian tubes: alveolar and secretive cells. These study shows the metabolic processes in pig oviductal epithelial cells associated with the activation of signaling pathways of amino acids metabolism and degradation during long-term in vitro culture. Oviductal epithelial cells from 45 colonies in the anestrous phase of the estrous cycle have been utilized in this study. RNA extract from the OEC primary cultures was pooled after 24h, 7days, 15 days and 30 days from the beginning of culture and the transcriptome investigated by Affymetrix® Porcine Gene 1.1 ST. From the whole transcript that consisted of 2009 different genes, 1537 were upregulated and 995 were downregulated after 7 days of culture, 1471 were upregulated and 1061 were downregulated after 15 days of culture and 1329 were upregulated and 1203 were downregulated after 30 days of culture. The results of these studies provide, for the first time, information on the activation of metabolic pathways of amino acids such as valine, leucine, isoleucine, cysteine, and methionine in the investigated tissue. They also indicate genes that may be OECs-specific genetic markers that are expressed or upregulated during long-term in vitro culture.

Keywords: pig; oviductal epithelial cells; in vitro culture (IVC); signaling pathways

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About the article

Received: 2017-11-14

Accepted: 2017-12-30

Published Online: 2018-01-31

Published in Print: 2018-01-01


Citation Information: Medical Journal of Cell Biology, Volume 6, Issue 1, Pages 18–26, ISSN (Online) 2544-3577, DOI: https://doi.org/10.2478/acb-2018-0004.

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© 2018 Wiesława Kranc et al., published by De Gruyter Open. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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