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Acta Medica Martiniana

The Journal of Comenius University in Bratislava

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1335-8421
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Non-Invasive Fetal Sex Determination Using SRY Specific Primers and Sybrgreen Real Time PCR

I. SvecovA
  • Clinic of Gynecology and Obstetrics, Comenius University and University Hospital, Martin, Slovak Republic
  • Other articles by this author:
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/ E. Jezkova
  • Clinic of Gynecology and Obstetrics, Comenius University and University Hospital, Martin, Slovak Republic
  • Department of Molecular Biology, Jessenius Faculty of Medicine, Comenius University and University Hospital, Martin, Slovak Republic
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ I Hudecova
  • Clinic of Gynecology and Obstetrics, Comenius University and University Hospital, Martin, Slovak Republic
  • Department of Molecular Biology, Jessenius Faculty of Medicine, Comenius University and University Hospital, Martin, Slovak Republic
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ T. Burjanivova
  • Clinic of Gynecology and Obstetrics, Comenius University and University Hospital, Martin, Slovak Republic
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  • De Gruyter OnlineGoogle Scholar
/ K. Biskupska-Bodova
  • Clinic of Gynecology and Obstetrics, Comenius University and University Hospital, Martin, Slovak Republic
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/ J. Danko
  • Clinic of Gynecology and Obstetrics, Comenius University and University Hospital, Martin, Slovak Republic
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  • De Gruyter OnlineGoogle Scholar
/ Z. Lasabová
  • Department of Molecular Biology, Jessenius Faculty of Medicine, Comenius University and University Hospital, Martin, Slovak Republic
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  • De Gruyter OnlineGoogle Scholar
Published Online: 2011-10-05 | DOI: https://doi.org/10.2478/v10201-011-0014-8

Non-Invasive Fetal Sex Determination Using SRY Specific Primers and Sybrgreen Real Time PCR

Presence of fetal cells and circulating free fetal DNA and RNA in maternal circulation represents the basic concept in developement of non-invasive prenatal diagnostic methods based on molecular biology and genetics. We introduced new methods for free fetal DNA isolation and detection in maternal circulation via DNA isolation from maternal plasma using real-time PCR SYBRGreen targeting and newly designed primers focused in SRY sequence. We determined gender in 46 singleton pregnancies, 22 boys and 24 girls and assessed the analytical and clinical validity. We reached 95.45% sensitivity and 95.83% specificity. We suggest improvements in molecular-biological procedures in the discussion, which could be used in studies of clinical utility of non-invasive prenatal diagnosis (NIPD) in decreasing amount of invasive procedures unnecessarily performed.

Keywords: non-invasive prenatal diagnosis; SRY; real-time PCR; fetal sex determination

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About the article


Published Online: 2011-10-05

Published in Print: 2011-08-01


Citation Information: Acta Medica Martiniana, Volume 11, Issue 2, Pages 26–30, ISSN (Print) 1335-8421, DOI: https://doi.org/10.2478/v10201-011-0014-8.

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