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Acta Medica Martiniana

The Journal of Comenius University in Bratislava

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Our Experiences With B Cell Clonality Analysis Using Multiplex Pcr Amplification in Paraffin-Embedded Tissue

L. Stanek
  • Institute of Pathology. 1 * Faculty of Medicine. Charles University and General Faculty Hospital in Prague. Czech IP.m.Klin
  • Other articles by this author:
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/ S. Llsova
  • Institute of Pathology. 1 * Faculty of Medicine. Charles University and General Faculty Hospital in Prague. Czech IP.m.Klin
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ D. Tvrdlk
  • Institute of Pathology. 1 * Faculty of Medicine. Charles University and General Faculty Hospital in Prague. Czech IP.m.Klin
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2014-05-30 | DOI: https://doi.org/10.2478/acm-2014-0001


The clonal determination of B-cell lymphoproliferative diseases by immunoglobulin heavy chain (IgH) rearrangement by polymerase chain reaction (PCR) is widely used. In this study we report our experiences with B cell clonality analysis using multiplex PCR amplification followed by heteroduplex analysis, which was found to be essential for the efficient resolution of monoclonal bands within polyclonal backgrounds, in formalin-fixed paraffinembedded tissue.

Keywords: monoclonality; PCR; IgH; paraffin-embedded tissue; heteroduplex


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About the article

Received: 2014-02-02

Accepted: 2014-04-22

Published Online: 2014-05-30

Published in Print: 2014-05-01

Citation Information: Acta Medica Martiniana, Volume 14, Issue 1, Pages 5–13, ISSN (Online) 1335-8421, DOI: https://doi.org/10.2478/acm-2014-0001.

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© by L. Stanek. This article is distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. BY-NC-ND 3.0

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