Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Acta Pharmaceutica

The Journal of Croatian Pharmaceutical Society

4 Issues per year


IMPACT FACTOR 2016: 1.288
5-year IMPACT FACTOR: 1.600

CiteScore 2016: 1.55

SCImago Journal Rank (SJR) 2016: 0.353
Source Normalized Impact per Paper (SNIP) 2016: 0.854

Open Access
Online
ISSN
1846-9558
See all formats and pricing
More options …
Volume 58, Issue 2 (Jun 2008)

Issues

Antioxidant and free radical scavenging potential of Citrullus colocynthis (L.) Schrad. methanolic fruit extract

Sunil Kumar
  • Department of Pharmaceutical Sciences, Kurukshetra University, Kurukshetra-136119 Haryana, India
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Dinesh Kumar
  • Department of Pharmaceutical Sciences, Kurukshetra University, Kurukshetra-136119 Haryana, India
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Man Jusha
  • Department of Pharmaceutical Sciences, Kurukshetra University, Kurukshetra-136119 Haryana, India
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Kamal Saroha
  • Department of Pharmaceutical Sciences, Kurukshetra University, Kurukshetra-136119 Haryana, India
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Nidhan Singh / Bhoodev Vashishta
Published Online: 2008-05-30 | DOI: https://doi.org/10.2478/v10007-008-0008-1

Antioxidant and free radical scavenging potential of Citrullus colocynthis (L.) Schrad. methanolic fruit extract

Citrullus colocynthis (L.) Schrad. (Cucurbitaceae) is a medicinal plant traditionally used as an abortifacient and to treat constipation, oedema, bacterial infections, cancer and diabetes. Preliminary phytochemical screening of the plant showed the presence of large amounts of phenolics and flavonoids. Subsequent quantification showed the presence of 0.74% (m/m) phenolics (calculated as gallic acid) and 0.13% (m/m) flavonoids calculated as catechin equivalents per 100 g of fresh mass. The presence of phenolic compounds prompted us to evaluate its antioxidant activity. In the present study, methanolic fruit extract of C. colocynthis was screened to evaluate its freeradical scavenging effect. The highest antioxidant and free radical scavenging ability of the fruit extract was observed at a concentration of 2500 μg mL-1.

Antioksidativni potencijal i sposobnost hvatanja slobodnih radikala metanolnog ekstrakta plodova Citrullus colocynthis (L.) Schrad.

Citrullus colocynthis (L.) Schrad. (Cucurbitaceae) je ljekovita biljka koja se tradicionalno upotrebljava kao abortiv i za liječenje konstipacije, edema, bakterijskih infekcija, karcinoma i dijabetesa. Preliminarno fitokemijsko pretraživanje ukazalo je na prisutnost velikih količina fenola i flavonoida. Udio fenola bio je 0,74% (preračunato na galnu kiselinu), a flavonoida 0,13% preračunato na ekvivalente katehina na 100 g svježe mase. Zbog prisutnosti fenolnih spojeva ispitivano je antioksidativno djelovanje i sposobnost hvatanja slobodnih radikala metanolnog ekstrakta plodova. Koncentracija 2500 μg mL-1 imala je najjači učinak.

Keywords: Citrullus colocynthis fruit; Cucurbitaceae; methanolic extract; antioxidants; free radical scavenging

Keywords: plod biljke Citrullus colocynthis (Cucurbitaceae); metanolni ekstrakt; antioksidansi; hvatanje slobodnih radikala

  • B. Halliwell and J. M. C. Gutteridge, Free Radicals in Biology and Medicine, 3rd ed., Oxford University Press, Oxford 1999.Google Scholar

  • T. Finkel and N. J. Holbrook, Oxidants, oxidative stress and the biology of aging, Nature 408 (2000) 239-247; DOI:10.1038/35041687.CrossrefGoogle Scholar

  • T. P. A. Devasagayam, J. C. Tilak, K. K. Boloor, K. S. Sane, S. S. Ghaskadbi and R. D. Lele, Review: Free radicals and antioxidants in human health: Current status and future prospects, J. Assoc. Phys. India 52 (2004) 794-804.Google Scholar

  • A. L. Miller, Antioxidant flavonoids: structure, function and clinical usage, Alt. Med. Rev. 1 (1996) 103-111.Google Scholar

  • A. Delazar, S. Gibbons, A. R. Kosari, H. Nazemiyeh, M. Modarresi, L. Nahar and S. D. Sarker, Flavone C-glycosides and cucurbitacin glycosides from Citrullus colocynthis, DARU 14 (2006) 109-114.Google Scholar

  • V. L. Singleton and J. A. Rossi, Colorimetry of total phenolics with phosphomolybdic phosphotungstic acid reagents, Am. J. Enol. Vitic. 16 (1965) 144-158.Google Scholar

  • D. Marinova, F. Ribarova and M. Atanasova, Total phenolics and flavonoids in Bulgarian fruits and vegetables, J. Univ. Chem. Tech. Metall. 40 (2005) 255-260.Google Scholar

  • N. Sreejayan and M. N. A. Rao, Free radical scavenging activity of curcuminoids, Drug Res. 46 (1996) 169-171.Google Scholar

  • S. M. Klein, G. Cohen and A. I. Cederbaum, Production of formaldehyde during metabolism of dimethylsulphoxide by hydroxyl radical generating system, Biochemistry 20 (1981) 6006-6012.CrossrefGoogle Scholar

  • I. Gülçin, H. A. Alici and M. Cesur, Determination of in vitro antioxidant and radical scavenging activities of propofol, Chem. Pharm. Bull. 53 (2005) 281-285; DOI: 10.1248/cpb.53.281.CrossrefGoogle Scholar

  • M. Nishikimi, N. A. Rao and K. Yagi, The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen, Biochem. Biophys. Res. Commun. 46 (1972) 849-853.PubMedGoogle Scholar

  • R. Govindarajan, S. Rastogi, M. Vijayakumar, A. Shirwaikar, A. K. Rawat, S. Mehrotra and P. Pushpangadan, Studies on antioxidant activities of Desmodium gangeticum, Biol. Pharm. Bull. 26 (2003) 1424-1427; DOI: 10.1248/bpd.26.1424.CrossrefPubMedGoogle Scholar

  • L. W. Aurand, N. H. Boonme and G. G. Gidding, Superoxide and singlet oxygen in milk lipid peroxidation, J. Dairy Sci. 60 (1977) 363-369.Google Scholar

  • A. E. Hagerman, K. M. Riedl, G. A. Jones, K. N. Sovik, N. T. Ritchard, P. W. Hartzfeld and T. L. Riechel, High molecular weight plant polyphenolics (tannins) as biological antioxidants, J. Agric. Food Chem. 46 (1998) 1887-1892; DOI: 10.1021/jf970975b.CrossrefGoogle Scholar

About the article


Published Online: 2008-05-30

Published in Print: 2008-06-01


Citation Information: Acta Pharmaceutica, ISSN (Online) 1846-9558, ISSN (Print) 1330-0075, DOI: https://doi.org/10.2478/v10007-008-0008-1.

Export Citation

This content is open access.

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

[2]
Susan Azizi, Rosfarizan Mohamad, Raha Abdul Rahim, Reza Mohammadinejad, and Arbakariya Bin Ariff
International Journal of Biological Macromolecules, 2017, Volume 104, Page 423
[4]
Yead Morshed Nibir, Ahmed Faisal Sumit, Anwarul Azim Akhand, Nazmul Ahsan, and Mohammad Shahnoor Hossain
Asian Pacific Journal of Tropical Biomedicine, 2017, Volume 7, Number 4, Page 352
[5]
Saeed Mehrzadi, Asie Shojaii, Sogol Attari Pur, and Manijeh Motevalian
Journal of Evidence-Based Complementary & Alternative Medicine, 2016, Volume 21, Number 4, Page NP31
[6]
Debasis Nayak, Sarbani Ashe, Pradipta Ranjan Rauta, and Bismita Nayak
Journal of Applied Biomedicine, 2017, Volume 15, Number 2, Page 119
[7]
Yogesh A. Kulkarni and Mayuresh S. Garud
Biomedicine & Pharmacotherapy, 2016, Volume 83, Page 122
[8]
Sangeeta Sankhalkar and Vrunda Vernekar
Pharmacognosy Research, 2016, Volume 8, Number 1, Page 16
[9]
Monica Rosa Bertao, Milena Cristina Moraes, Dario Abel Palmieri, Luciana Pereira Si, and Regildo Marcio Goncalves
Research Journal of Medicinal Plants, 2016, Volume 10, Number 4, Page 265
[10]
Batol Barghamdi, Fereshteh Ghorat, Khairollah Asadollahi, Kourosh Sayehmiri, Robab Peyghambari, and Ghobad Abangah
Journal of Pharmacy and Bioallied Sciences, 2016, Volume 8, Number 2, Page 130
[11]
Daniil Olennikov, Nadezhda Chirikova, Zhanna Okhlopkova, and Ismayl Zulfugarov
Molecules, 2013, Volume 18, Number 11, Page 14105
[12]
Mufidah Murdifin, Ermina Pakki, Abdul Rahim, Syatrianti A. Syaiful, Ismail ., Yayu M. Evary, and M. Akbar Bahar
Asian Journal of Plant Sciences, 2015, Volume 14, Number 2, Page 59
[13]
Fowzia Akter, Mozammel Haque, Jahirul Islam, Asiqur Rahaman, Sujan Bhowmick, and Shahdat Hossain
Advances in Alzheimer's Disease, 2015, Volume 04, Number 03, Page 78
[14]
Thiago Buno Lima Prando, Lorena Neris Barboza, Francielly Mourão Gasparotto, Valdinei de Oliveira Araújo, Cleide Adriane Slgnor Tirloni, Lauro Mera de Souza, Emerson Luiz Botelho Lourenço, and Arquimedes Gasparotto Junior
Journal of Ethnopharmacology, 2015, Volume 174, Page 369
[15]
Echaimaa Chekroun, Nabila Benariba, Houria Adida, Asma Bechiri, Rachid Azzi, and Rabeh Djaziri
Asian Pacific Journal of Tropical Disease, 2015, Volume 5, Number 8, Page 632
[16]
Mojtaba Heydari, Kaynoosh Homayouni, Mohammad Hashem Hashempur, and Mesbah Shams
Journal of Diabetes, 2016, Volume 8, Number 2, Page 246
[17]
Priti Gupta, Ira Bhatnagar, Se-Kwon Kim, Ajay Kumar Verma, and Anubhuti Sharma
Asian Pacific Journal of Tropical Biomedicine, 2014, Volume 4, Page S665
[18]
Nabila Benariba, Rabeh Djaziri, Wafaa Bellakhdar, Nacera Belkacem, Marcel Kadiata, Willy J. Malaisse, and Abdullah Sener
Asian Pacific Journal of Tropical Biomedicine, 2013, Volume 3, Number 1, Page 35
[19]
Narayanaswamy Tamilselvan, Thirunavukkarasu Thirumalai, Prabakar Shyamala, and Ernest David
Journal of Acute Disease, 2014, Volume 3, Number 2, Page 85
[20]
Abin Sebastian and M.N.V. Prasad
Journal of Photochemistry and Photobiology B: Biology, 2014, Volume 137, Page 135
[21]
Abdullah I. Hussain, Hassaan A. Rathore, Munavvar Z.A. Sattar, Shahzad A.S. Chatha, Satyajit D. Sarker, and Anwar H. Gilani
Journal of Ethnopharmacology, 2014, Volume 155, Number 1, Page 54
[22]
Udoamaka F. Ezuruike and Jose M. Prieto
Journal of Ethnopharmacology, 2014, Volume 155, Number 2, Page 857
[24]
Mohammad Shokrzadeh, Aroona Chabra, Farshad Naghshvar, and Amirhossein Ahmadi
The Scientific World Journal, 2013, Volume 2013, Page 1
[25]
T.A.F. Eissa, O.M. Palomino, M.E. Carretero, and M.P. Gómez-Serranillos
Journal of Ethnopharmacology, 2014, Volume 151, Number 1, Page 317
[26]
Ahmad Oryan, Mohammad Hashemnia, Ahmad-Reza Hamidi, and Adel Mohammadalipour
Asian Pacific Journal of Tropical Disease, 2014, Volume 4, Number 2, Page 125
[27]
Soodabeh Saeidnia and Mohammad Abdollahi
Toxicology and Applied Pharmacology, 2013, Volume 273, Number 3, Page 442
[28]
Maria G. Miguel, Leonor Faleiro, Maria D. Antunes, Smail Aazza, Joana Duarte, and Ana R. Silvério
Food and Chemical Toxicology, 2013, Volume 56, Page 136
[29]
Abdullah I. Hussain, Hassaan A. Rathore, Munavvar Z.A. Sattar, Shahzad A.S. Chatha, Fiaz ud din Ahmad, Ashfaq Ahmad, and Edward J. Johns
Industrial Crops and Products, 2013, Volume 45, Page 416
[30]
Geun-Hye Park, Jin-Tae Lee, and Bong-Jeun An
The Korea Journal of Herbology, 2012, Volume 27, Number 3, Page 23
[33]
Varahalarao Vadlapudi and D.S.V.G.K. Kaladhar
Asian Pacific Journal of Tropical Disease, 2012, Volume 2, Page S26
[34]
D. N. Olennikov, L. M. Tankhaeva, A. V. Rokhin, and S. V. Agafonova
Chemistry of Natural Compounds, 2012, Volume 48, Number 3, Page 396
[35]
Roja Rahimi, Gholamreza Amin, and Mohammad Reza Shams Ardekani
The Journal of Alternative and Complementary Medicine, 2012, Volume 18, Number 6, Page 551
[36]
Bolanle Ibrahim, Abimbola Sowemimo, Anzel van Rooyen, and Maryna Van de Venter
Journal of Ethnopharmacology, 2012, Volume 141, Number 1, Page 282
[37]
Shanti Bhushan Mishra, Amita Verma, Alok Mukerjee, and M Vijayakumar
Asian Pacific Journal of Tropical Medicine, 2011, Volume 4, Number 9, Page 689
[39]
Taghreed A. Ibrahim, Hala M. El-Hefnawy, and Atef A. El-Hela
Natural Product Research, 2010, Volume 24, Number 16, Page 1537
[40]
Babasaheb P. Bandgar, Shrikant S. Gawande, Suchita C. Warangkar, and Jalinder V. Totre
Bioorganic & Medicinal Chemistry, 2010, Volume 18, Number 10, Page 3618
[41]
N.S. Gill, K. Dhiman, J. Bajwa, P. Sharma, and S. Sood
International Journal of Pharmacology, 2010, Volume 6, Number 5, Page 652

Comments (0)

Please log in or register to comment.
Log in