Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Acta Pharmaceutica

The Journal of Croatian Pharmaceutical Society

4 Issues per year


IMPACT FACTOR 2016: 1.288
5-year IMPACT FACTOR: 1.600

CiteScore 2016: 1.55

SCImago Journal Rank (SJR) 2016: 0.353
Source Normalized Impact per Paper (SNIP) 2016: 0.854

Open Access
Online
ISSN
1846-9558
See all formats and pricing
More options …
Volume 58, Issue 3 (Sep 2008)

Issues

Development and validation of spectrophotometric methods for determination of ceftazidime in pharmaceutical dosage forms

Basavaraj Hiremath / Bennikallu Mruthyunjayaswamy
Published Online: 2008-12-22 | DOI: https://doi.org/10.2478/v10007-008-0017-0

Development and validation of spectrophotometric methods for determination of ceftazidime in pharmaceutical dosage forms

Two spectrophotometric methods for the determination of ceftazidime (CFZM) in either pure form or in its pharmaceutical formulations are described. The first method is based on the reaction of 3-methylbenzothiazolin-2-one hydrazone (MBTH) with ceftazidime in the presence of ferric chloride in acidic medium. The resulting blue complex absorbs at λmax 628 nm. The second method describes the reaction between the diazotized drug and N-(1-naphthyl)ethylenediamine dihydrochloride (NEDA) to yield a purple colored product with λmax at 567 nm. The reaction conditions were optimized to obtain maximum color intensity. The absorbance was found to increase linearly with increasing the concentration of CFZM; the systems obeyed the Beer's law in the range 2-10 and 10-50 μg mL-1 for MBTH and NEDA methods, resp. LOD, LOQ and correlation coefficient values were 0.15, 0.79 and 0.50, 2.61. No interference was observed from common excipients present in pharmaceutical formulations. The proposed methods are simple, sensitive, accurate and suitable for quality control applications.

Razvoj i validacija spektrofotometrijskih metoda za određivanje ceftazidima u farmaceutskim doziranim pripravcima

Razvijene su dvije spektrofotometrijske metode za određivanje ceftazidima (CFZM), čistog ili u farmaceutskim pripravcima. Prva metoda se temelji na reakciji 3-metilbenzotiazolin-2-on hidrazona (MBTH) sa ceftazidimom u prisutnosti željezovog(III) klorida u kiselom mediju. Nastaje plavi kompleks s maksimumom apsorpcije pri λmax 628 nm. Druga metoda se temelji na reakciji između diazotiranog lijeka i N-(1-naftil)etilendiamin dihidroklorida (NEDA), pri čemu nastaje ljubičasti produkt s λmax pri 567 nm. Reakcijski uvjeti su optimirani da se dobije maksimalni intenzitet boje. Apsorbancija raste linearno s porašću koncentracije CFZM; sustavi slijede Beerov zakon u koncentracijskom području 2-10 za MBTH metodu i 1050 μg mL-1 za NEDA metodu. LOD i LOQ te vrijednosti korelacijskog koeficijenta su 0,15, 0,79 i 0,50, 2,61. Uobičajene pomoćne tvari ne smetaju određivanju ceftazidima. Predložene metode su jednostavne, osjetljive, točne i pogodne za primjenu u kontroli kvalitete.

Keywords: ceftazidime; diazotization; spectrophotometry; pharmaceutical formulation

Keywords: ceftazidim; diazotacija; spektrofotometrija; farmaceutski pripravak

  • Martindale - The Complete Drug Reference, 33rd ed., Pharmaceutical Press, London 2002, p. 174.Google Scholar

  • Merck Index, 13th ed., Merck and Co. Inc., Whitehouse Station 2001, p. 160.Google Scholar

  • B. G. Katzung, Basic and Clinical Pharmacology, 2nd ed., Appleton and Lange, New York 1987, pp. 522-525.Google Scholar

  • S. D. Hanes, V. L. Herring and G. C. Wood, Alternative method for determination of ceftazidime in plasma by high-performance liquid chromatography, J. Chromatogr. B Biomed. Sci. Appl. 719 (1998) 245-250; DOI: 10.1016/S0378-4347(98)00407-1.CrossrefGoogle Scholar

  • T. H. Doadrio, A. K. Rumelin and U. F. Orenga, Ceftazidime determination in serum by high-pressure liquid chromatography, Arzneim. Forsch. 54 (2004) 320-322.Google Scholar

  • A. F. M. El-Walily, A. A. Gazy, S. F. Belal and E. F. Khamis, Quantitative determination of some thiazole cephalosplorins through complexation with palladium(II) chloride, J. Pharm. Biomed. Anal. 22 (2000) 385-392; DOI: 10.1016/S0731-7085(99)00281-2.CrossrefGoogle Scholar

  • The United States Pharmacopoeia 25, National Formulary 20, USP Convention, Rockville 2002, pp. 354-356.Google Scholar

  • Indian Pharmacopoeia, Controller of Publications, Delhi 1996, pp. 149-150.Google Scholar

  • V. Rodenas, M. S. Garcia, C. Sanchez-Pedreno and M. I. Albero, Spectrophotometric methods for the determination of cephradine or ceftazidime in human urine using batch and flow-injection procedures, J. Pharm. Biomed. Anal. 15 (1997) 1687-1693; DOI: 10.1016/S0731-7085(96)01963-2.CrossrefGoogle Scholar

  • K. R. Khadiga, I. L. Bebawy and L. I. Abdel-Fattah, Stability-indicating spectrophotometric and densitometric methods for determination of some cephalosporins, J. AOAC. Int. 81 (1998) 386-396.Google Scholar

  • R. C. Vamsi, B. H. Sarojini, A. R. Geetha, M. O. Shobana, R. D. Umarani, L. N. Kavitha, S. N. Meyyanathan and B. S. Suresh, Spectrophotometric method for the determination of ceftazidime sodium, East. Pharm. 43 (2000) 143-144.Google Scholar

  • E. H. Abdellatef, A. A. Shalaby, M. H. Elsaid and M. M. Ayad, Colorimetric and titrimetric methods for determination of some cephalosporins in their pure and dosage forms, Sci. Pharm. 68 (2000) 263-273.Google Scholar

  • E. S. Marwa, M. N. Elbolkiny and M. H. Khalil, Spectrophotometric determination of acyclovir, ceftazidime pentahydrate, famotidine and isoxsuprine hydrochloride by ternary complex formation with eosin and Cu(II), Chin. Pharm. J. 55 (2003) 481-490.Google Scholar

  • E. S. Marwa, A. Shalaby, M. N. Elbolkiny and M. H. Khalil, Spectrophotometric determination of cefepime hydrochloride, cefoperazone sodium, ceftazidime pentahydrate, cefuroxime sodium and etamsylate using ammonium molybdate, Sci. Pharm. 71 (2003) 211-228.Google Scholar

  • B. Hiremath and B. H. M. Mruthyunjayaswamy, Development and validation of spectrophotometric methods for the determination of cefetamet in pharmaceutical dosage forms, Chin. J. Chem. 25 (2007) 1827-1831; DOI: wiley.com/10.1002/cjoc.200790337.CrossrefGoogle Scholar

  • B. Hiremath and B. H. M. Mruthyunjayaswamy, Development and validation of spectrophotometric methods for determination of ceftriaxone sodium in pharmaceutical dosage forms, J. Indian Council Chem. 23 (2006) 111-115.Google Scholar

  • D. Harvey, Modern Analytical Chemistry, 1st ed., McGraw-Hill Book Co, Singapore, 2000, pp. 38-44.Google Scholar

  • International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use, ICH Harmonised Tripartite Guidelines, Validation of Analytical Procedures: Text and Methodology Q2 (R1), Current Step 4 version, Nov. 1996, Geneva, Nov. 2005.Google Scholar

About the article


Published Online: 2008-12-22

Published in Print: 2008-09-01


Citation Information: Acta Pharmaceutica, ISSN (Online) 1846-9558, ISSN (Print) 1330-0075, DOI: https://doi.org/10.2478/v10007-008-0017-0.

Export Citation

This content is open access.

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

[3]
Noura Hemdan Abou-Taleb, Dalia Rashad El-Wasseef, Dina Tawfik El-Sherbiny, and Saadia Mohamed El-Ashry
Luminescence, 2016, Volume 31, Number 3, Page 856
[4]
Shiva Mahramyari, Eslam Pourbasheer, Alireza Banaei, Mohammad Reza Ganjali, and Parviz Norouzi
RSC Adv., 2014, Volume 4, Number 77, Page 41039
[6]
Derya Tarinc and Aysegul Golcu
Journal of Analytical Chemistry, 2012, Volume 67, Number 2, Page 144
[7]
B. A. Moussa, M. F. Mohamed, and N. F. Youssef
Journal of Applied Spectroscopy, 2011, Volume 77, Number 6, Page 869

Comments (0)

Please log in or register to comment.
Log in