Jump to ContentJump to Main Navigation
Show Summary Details
In This Section

Acta Pharmaceutica

The Journal of Croatian Pharmaceutical Society

4 Issues per year


IMPACT FACTOR 2016: 1.288
5-year IMPACT FACTOR: 1.600

CiteScore 2016: 1.55

SCImago Journal Rank (SJR) 2015: 0.485
Source Normalized Impact per Paper (SNIP) 2015: 0.960

Open Access
Online
ISSN
1846-9558
See all formats and pricing
In This Section
Volume 59, Issue 3 (Sep 2009)

Issues

Chemical composition, antioxidant, anti-elastase, and anti-inflammatory activities of Illicium anisatum essential oil

Ji-Young Kim
  • Research Group for Cosmetic Materials Jeju Biodiversity Research Institute (JBRI), Jeju Hi-Tech Industry Development Institute (HiDI), Jeju 699-943, Korea
/ Sang-Suk Kim
  • Department of Chemistry, Cheju National University, Jeju 690-756, Korea
/ Tae-Heon Oh
  • Department of Chemistry, Cheju National University, Jeju 690-756, Korea
/ Jong Baik
  • Department of Chemistry, Cheju National University, Jeju 690-756, Korea
/ Gwanpil Song
  • Research Group for Cosmetic Materials Jeju Biodiversity Research Institute (JBRI), Jeju Hi-Tech Industry Development Institute (HiDI), Jeju 699-943, Korea
/ Nam Lee
  • Department of Chemistry, Cheju National University, Jeju 690-756, Korea
/ Chang-Gu Hyun
  • Research Group for Cosmetic Materials Jeju Biodiversity Research Institute (JBRI), Jeju Hi-Tech Industry Development Institute (HiDI), Jeju 699-943, Korea
Published Online: 2009-10-09 | DOI: https://doi.org/10.2478/v10007-009-0022-y

Chemical composition, antioxidant, anti-elastase, and anti-inflammatory activities of Illicium anisatum essential oil

The essential oil of air-dried Illicium anisatum (Illiciaceae), obtained by hydrodistillation was analyzed by gas chromatography-mass spectrometry (GC-MS). Fifty-two components were identified in the essential oil and the main component was eucalyptol (21.8 %). The antioxidant and anti-elastase activities of the essential oil were also investigated; the essential oil exhibited moderate DPPH scavenging and anti-elastase activities. To clarify the mechanism of the anti-inflammatory activities of I. anisatum essential oil (IAE), we evaluated whether it could modulate the production of nitric oxide (NO) and prostaglandin E2 (PGE2) by activated macrophages. The results indicate that IAE is an effective inhibitor of LPS-induced NO and PGE2 production in RAW 264.7 cells. These inhibitory activities were accompanied by dose-dependent decreases in the expression of iNOS and COX-2 proteins and iNOS and COX-2 mRNA. In order to determine whether IAE can be safely applied to human skin, the cytotoxic effects of IAE were determined by colorimetric MTT assays in human dermal fibroblast and keratinocyte HaCaT cells. IAE exhibited low cytotoxicity at 100 μg mL-1. Based on these results, we suggest that IAE may be considered an anti-aging and anti-inflammatory candidate for cosmetic materials, but additional in vitro and in vivo tests have to be performed to prove its safety and efficacy.

Kemijski sastav, antioksidativno djelovanje, inhibicija elastaze i protuupalno djelovanje eteričnog ulja biljke Illicium anisatum

Eterično ulje biljke Illicium anisatum dobiveno destilacijom vodenom parom analizirano je plinskom kromatografijom-spektrometrijom masa (GC-MS). Identificirane su pedeset i dvije komponente eteričnog ulja, a glavna komponenta je eukaliptol (21,8 %). Ispitivanje antioksidativnog djelovanja te djelovanja na elastazu ukazuju na umjerenu spo-sobnost hvatanja DPPH radikala i inhibicije elastaze. Kako bi se objasnio mehanizam protuupalnog djelovanja eteričnog ulja I. anisatum (IAE), ispitan je učinak na moduliranje produkcije dušikovog(II) oksida (NO) i prostaglandina E2 (PGE2) iz aktiviranih makrofaga. Rezultati ukazuju da je IAE učinkovit inhibitor LPS-inducirane produkcije NO i PGE2 u RAW 264.7 stanicama. Inhibitorno djelovanje popraćeno je smanjenjem ekspresije iNOS i COX-2 proteina i iNOS i COX-2 mRNA. Kako bi se odredilo može li se IAE sigurno primijeniti na ljudsku kožu, citotoksični učinci IAE određeni su kolorimetrijskim MTT testom u humanim dermalnim fibroblastima i keratinocitima HaCaT. IAE je pokazao nisku citotoksičnost pri koncentraciji 100 μg mL-1. Temeljem ovih rezultata IAE se može smatrati potencijalnim sredstvom protiv starenja i protuupalnim sredstvom u kozmetičkim pripravcima. Međutim, dodatni in vitro i in vivo testovi nužni su za potvrdu njegove sigurnosti i učinkovitosti.

Keywords: Illicium anisatum; Illicium religiosum; Illicium japonicum (Illiciaceae); chemical composition; cosmetics; DPPH; elastase; inflammation

Keywords: Illicium anisatum; Illicium religiosum; Illicium japonicum; kemijski sastav; kozmetika; DPPH; elastaza; upala

  • P. Minodier, P. Pommier, E. Moulène, K. Retornaz, N. Prost and L. Deharo, Star anise poisoning in infants, Arch. Pediatr. 10 (2003) 619-621; DOI: 10.1016/S0929-693X(03)00274-4. [PubMed] [Crossref]

  • H. Y. Kim and J. H. Oh, Screening of Korean forest plants for rat lens aldose reductase inhibition, Biosci. Biotechnol. Biochem. 63 (1999) 184-188; DOI: 10.1271/bbb.63.184. [Crossref] [PubMed]

  • H. Y. Kim and K. Kim, Protein glycation inhibitory and antioxidative activities of some plant extracts in vitro, J. Agric. Food Chem. 51 (2003) 1586-1591; DOI: 10.1021/jf020850t. [Crossref]

  • H. Y. Kim and M. H. Kang, Screening of Korean medicinal plants for lipase inhibitory activity, Phytother. Res. 19 (2005) 359-361; DOI: 10.1002/ptr.1592. [Crossref] [PubMed]

  • M. Shibuya, K. Abe, Y. Nakahashi and S. Kubota, Phenolic components from leaf oil of Illicium anisatum L., Chem. Pharm. Bull. 26 (1978) 2671-2673; DOI: 10.1248/cpb.26.2671. [Crossref]

  • Y. Kudo, J. I. Oka and K. Yamada, Anisatin, a potent GABA antagonist, isolated from Illicium anisatum, Neurosci. Lett. 25 (1981) 83-88; DOI: 10.1016/0304-3940(81)90105-1. [Crossref] [PubMed]

  • J. Y. Kor and D. W. Choi, Quantitative determination of glutamate mediated cortical neuronal injury in cell culture by lactate dehydrogenase efflux assay, J. Neurosci. Meth. 20 (1987) 83-89; DOI: 10.1016/0165-0270(87)90041-0. [Crossref]

  • M. Fernández, J. C. Ríos, A. Jos and G. Repetto, Comparative cytotoxicity of alachlor on RTG-2 trout and SH-SY5Y human cells, Arch. Environ. Contam. Toxicol. 51 (2006) 515-520; DOI: 10.1007/s00244-005-0086-8. [Crossref]

  • M. M. Bradford, A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Anal. Biochem. 72 (1976) 248-254; DOI: 10.1016/0003-2697(76)90527-3. [PubMed] [Crossref]

  • W. B. Cook and A. S. Howard, The essential oil of Illicium anisatum Linn., Can. J. Chem. 44 (1966) 2461-2464; DOI: 10.1139/v66-371. [Crossref]

  • N. Mimica-Dukić, B. Bozin, M. Soković, B. Mihajlović and M. Matavulj, Antimicrobial and antioxidant activities of three Mentha species essential oils, Planta Med. 69 (2003) 413-419; DOI: 10.1055/s-2003-39704. [Crossref]

  • S. Kordali, A. Cakir, A. Mavi, H. Kilic and A. Yildirim, Screening of chemical composition and antifungal and antioxidant activities of the essential oils from three Turkish artemisia species, J. Agric. Food Chem. 53 (2005) 1408-1416; DOI: 10.1021/jf048429n. [Crossref]

  • R. Thirugnanasampandan, R. Jayakumar, V. Narmatha Bai, E. Martin and K. J. Rajendra Prasad, Antiacetylcholinesterase and antioxidant ent-Kaurene diterpenoid, melissoidesin from Isodon wightii (Bentham) H. Hara, Nat. Prod. Res. 22 (2008) 681-688; DOI: 10.1080/14786410801990625. [Crossref]

About the article


Published Online: 2009-10-09

Published in Print: 2009-09-01



Citation Information: Acta Pharmaceutica, ISSN (Online) 1846-9558, ISSN (Print) 1330-0075, DOI: https://doi.org/10.2478/v10007-009-0022-y. Export Citation

This content is open access.

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

[1]
Ram Kumar Sahu, Amit Roy, Munglu Matlam, Vinay Kumar Deshmukh, Jaya Dwivedi, and Arvind Kumar Jha
Research Journal of Medicinal Plant, 2013, Volume 7, Number 1, Page 1
[2]
Ivana Binic, Viktor Lazarevic, Milanka Ljubenovic, Jelena Mojsa, and Dusan Sokolovic
Evidence-Based Complementary and Alternative Medicine, 2013, Volume 2013, Page 1
[3]
Keng Hong Tan and Ritsuo Nishida
Journal of Insect Science, 2012, Volume 12, Number 56, Page 1
[4]
A Vijayakumar, V Duraipandiyan, B Jeyaraj, P Agastian, M Karunai Raj, and S Ignacimuthu
Asian Pacific Journal of Tropical Disease, 2012, Volume 2, Number 3, Page 190
[5]
Salud Pérez G, Miguel Zavala S., Lucina Arias G., and Miguel Ramos L.
Journal of Essential Oil Research, 2011, Volume 23, Number 5, Page 38
[6]
W.J. Yoon, J.Y. Moon, G. Song, Y.K. Lee, M.S. Han, J.S. Lee, B.S. Ihm, W.J. Lee, N.H. Lee, and C.G. Hyun
Food and Chemical Toxicology, 2010, Volume 48, Number 5, Page 1222
[7]
Barbara Adorjan and Gerhard Buchbauer
Flavour and Fragrance Journal, 2010, Volume 25, Number 6, Page 407

Comments (0)

Please log in or register to comment.
Log in