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Acta Pharmaceutica

The Journal of Croatian Pharmaceutical Society

4 Issues per year


IMPACT FACTOR 2016: 1.288
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1846-9558
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Volume 62, Issue 1 (Mar 2012)

Issues

Stability studies of chitosan-DNA-FAP-B nanoparticles for gene delivery to lung epithelial cells

Zohreh Mohammadi
  • Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
  • Nanobiotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Farid Dorkoosh
  • Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Saman Hosseinkhani / Tina Amini / Amir Rahimi / Abdolhossein Najafabadi
  • Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Morteza Tehrani
  • Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2012-04-03 | DOI: https://doi.org/10.2478/v10007-012-0008-z

Stability studies of chitosan-DNA-FAP-B nanoparticles for gene delivery to lung epithelial cells

A successful gene delivery system requires efficiency and stability during storage. Stability studies are imperative for nanomedicines containing biotechnological products such as plasmids and targeting peptides. Chitosan-DNA-FAP-B nanoparticles are novel non-viral vectors for specific gene delivery to the lung epithelial cells. In this study, the storage stability of chitosan-DNA-FAP-B nanoparticles at -20, 5 and 24 °C was examined. Size, zeta potential and transfection efficiency of these nano-particles in storage were also evaluated. Stability studies showed that chitosan-DNA-FAP-B nanoparticles were stable after 1 month when stored at -20 °C and retained their initial size, zeta potential and transfection efficiency. However, their stability was not desirable at 5 and 24 °C. Based on these results, it can be concluded that chitosan-DNA-FAP-B nanoparticles can be a promising candidate for gene delivery to lung epithelial cells with good storage stability at -20 °C during 1 month.

Ispitivanje stabilnosti kitozanskih nanočestica s DNA-FAP-B za gensku isporuku u epitelne stanice pluća

Dobar sustav za gensku isporuku mora biti učinkovit i stabilan prilikom skladištenja. Stoga su ispitivanja stabilnosti nužna za nonosustave s biotehnološkim produktima kao što su plazmidi i ciljni peptidi. Kitozanske nanočestice s DNA-FAP-B su novi nevirusni vektori za specifičnu gensku isporuku u epitelne stanice pluća. U radu je ispitana njihova stabilnost na -20, 5 i 24 °C, veličina čestica, zeta potencijal i transfekcijska učinkovitost. Dobiveni rezultati pokazuju da su kitozanske nanočestice s DNA-FAP-B stabilne nakon mjesec dana skladištenja na -20 °C i da zadržavaju početnu veličinu, zeta potencijal i sposobnost transfekcije, a nisu stabilne skladištenjem na 5 i 24 °C. Može se zaključ iti da su kitozanske nanočestice s DNA-FAP-B potencijalni kandidati za gensku isporuku u epitelne stanice pluća te da su dovoljno stabilne ako se skladište mjesec dana na -20 °C.

Keywords: chitosan; FAP-B; nanoparticle; stability; gene delivery

Keywords: kitozan; FAP-B; nanočestica; stabilnost; genska isporuka

  • S. Mansouria, Y. Cuieb, F. Winnikb, Q. Shia, P. Lavignea, M. Benderdoura, E. Beaumonta and J. C. Fernandes, Characterization of folate-chitosan-DNA nanoparticles for gene therapy, Biomaterials 27 (2006) 2060-2065; DOI: 10.1016/j.biomaterials.2005.09.020.CrossrefGoogle Scholar

  • Z. Cui and R. J. Mumper, Chitosan-based nanoparticles for topical genetic immunization, J. Control. Release 75 (2001) 409-419; DOI: 10.1016/s0168-3659(01)00407-2.CrossrefGoogle Scholar

  • F. C. MacLaughlin, R. J. Mumper, J. Wang, J. M. Tagliaferri, I. Gill, M. Hinchcliffe and A. P. Rolland, Chitosan and depolymerized chitosan oligomers as condensing carriers for in vivo plasmid delivery, J. Control. Release 56 (1998) 259-272; DOI: 10.1016/s0168-3659(98)00097-2.CrossrefGoogle Scholar

  • S. Safari, F. A. Dorkoosh, M. Soleimani, M. H. Zarrintan, H. Akbari, B. Larijani and M. Rafiee Tehrani, N-Diethylmethyl chitosan for gene delivery to pancreatic cancer cells and the relation between charge ratio and biologic properties of polyplexes via interpolations polynomial, Int. J. Pharm. 420 (2011) 350-357; DOI: 10.1016/j.ijpharm.2011.08.050.CrossrefWeb of ScienceGoogle Scholar

  • M. Abolhassani, M. Lagranderie, I. Caminshi, F. Romain, A. Balazuc, M. Wagner, M. Tanguy, L. Fiette, I. Sobhani, G. Milon and G. Marchal, Similar functional activity of dendritic cells recruited to the mesenteric lymph nodes of newborn and adult mice after the rectal delivery of Mycobacterium bovis BCG, Microbes Infect 8 (2006) 2341-2351; DOI: 10.1016/j.micinf.2006.03.022.CrossrefGoogle Scholar

  • Z. Mohammadi, M. Abolhassani, F. A. Dorkoosh, S. Hosseinkhani, K. Gilani, T. Amini, A. Rouholamini Najafabadi and M. Rafiee Tehrani, Preparation and evaluation of chitosan-DNA-FAP-B nanoparticles as a novel non-viral vector for gene delivery to the lung epithelial cells, Int. J. Pharm. 409 (2011) 307-313; DOI:10.1016/j.ijpharm.2011.02.043.CrossrefGoogle Scholar

  • Z. Mohammadi, F. A. Dorkoosh, S. Hosseinkhani, K. Gilani, T. Amini, A. Rouholamini Najafabadi and M. Rafiee Tehrani, In vivo transfection study of chitosan-DNA-FAP-B nanoparticles as a new non viral vector for gene delivery to the lung, Int. J. Pharm. 421 (2011) 183-188; DOI: 10.1016/j.ijpharm.2011.09.029.CrossrefWeb of ScienceGoogle Scholar

  • M. S. Muthu and S. Feng, Pharmaceutical stability aspects of nanomedicines, Nanomedicine 4 (2009) 857-860; DOI:10.2217/nnm.09.75.CrossrefWeb of ScienceGoogle Scholar

  • International Conference on Harmonization, ICH Q1A (R2), Stability Testing Guidelines: Stability Testing of New Drug Substances and Products, ICH step 5, CPMP/ICH/2736/99, London 2003.Google Scholar

  • F. Romain, A. Laqueyrerie, P. Militzer, P. Pescher, P. Chavarot, M. La-Granderie, G. Auregan, M. Gheorghiu and G. Marchal, Identification of a Mycobacterium bovis BCG 45/47-kilodalton antigen complex, an immunodominant target for antibody response after immunization with living bacteria, Infect. Immun 61 (1993) 742-750.Google Scholar

  • M. Elfinger, J. Geiger, G. Hasenpusch, S. Üzgün, N. Sieverling, M. K. Aneja, C. Maucksch and C. Rudolph, Targeting of the β2-adrenoceptor increases nonviral gene delivery to pulmonary epithelial cells in vitro and lungs in vivo, J. Control. Release 135 (2009) 234-241; DOI: 10.1016/j.jcourel.2009.01.012.CrossrefWeb of ScienceGoogle Scholar

  • H. Mao, K. Roy, V. Troung-Le, K. A. Janes, K. Y. Lin, Y. Wang, J. T. August and K. W. Leong, Chitosan-DNA nanoparticles as gene carriers: synthesis, characterization and transfection efficiency, J. Control. Release 70 (2001) 399-421; DOI: 10.1016/s0168-3659(00)00361-8.CrossrefGoogle Scholar

  • International Conference on Harmonization, ICH Q5C, Stability Testing of Biotechnological/Biological Products, ICH step 4, CPMP/ICH/138/95, London 1996.Google Scholar

  • T. Arakawa, S. J. Prestrelski, W. C. Kenney and J. F. Carpenter, Factors affecting short-term and long-term stabilities of proteins, Adv. Drug Deliver. Rev. 46 (2001) 307-326; DOI: 10.1016/s0169-409x(00)00144-7.CrossrefGoogle Scholar

  • G. Borchard, Chitosans for gene delivery, Adv. Drug Deliver. Rev. 52 (2001) 145-150; DOI: 10.1016/s0169-409x(01)00198-3.CrossrefGoogle Scholar

  • H. Okamoto, Y. Sakakura, K. Shiraki, K. Oka, S. Nishida, H. Todo, K. Iida and K. Danjo, Stability of chitosan-pDNA complex powder prepared by supercritical carbon dioxide process, Int. J. Pharm 290 (2005) 73-81; DOI: 10.1016/j.ijpharm.2004.11.026.CrossrefGoogle Scholar

  • W. Walther, U. Stein, C. Voss, T. Schmidt, M. Schleef and P. M. Schlag, Stability analysis for long-term storage of naked DNA: impact on nonviral in vivo gene transfer, Anal. Biochem 318 (2003) 230-235; DOI: 10.1016/s0003-2697(03)00244-6.CrossrefGoogle Scholar

  • D. M. Prazeres, G. N. M. Ferreira, G. A. Monteiro, C. L. Cooney and J. M. S. Cabral, Large-scale production of pharmaceutical-grade plasmid DNA for gene therapy: problems and bottlenecks, Trends Biotechnol. 17 (1999) 169-174; DOI: 10.1016/s0167-7799(98)01291-8.CrossrefPubMedGoogle Scholar

About the article


Published Online: 2012-04-03

Published in Print: 2012-03-01


Citation Information: Acta Pharmaceutica, ISSN (Online) 1846-9558, ISSN (Print) 1330-0075, DOI: https://doi.org/10.2478/v10007-012-0008-z.

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