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Acta Pharmaceutica

The Journal of Croatian Pharmaceutical Society

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Volume 63, Issue 4


Design and in vivo evaluation of emulgel formulations including green tea extract and rose oil

Evren Algin Yapar
  • Corresponding author
  • The Ministry of Health of Turkey, Turkish Medicines and Medical Devices Agency, Söðütözü Mahallesi, 2176, 06520 Çankaya-Ankara, Turkey
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/ Özge Ýnal
  • Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Ankara, 06100 Tandoðan-Ankara, Turkey
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/ M. Sedef Erdal
  • Department of Pharmaceutical Technology, Faculty of Pharmacy, Istanbul University, 34116 Beyazýt-Istanbul, Turkey
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Published Online: 2013-12-31 | DOI: https://doi.org/10.2478/acph-2013-0037


Prevention of skin aging and its treatment is an emerging field for development of new formulations in cosmetics. Accordingly, plant extracts with antioxidant properties are beneficial cosmetic ingredients for this purpose. This study was aimed at developing a stable and easily manufactured emulgel including green tea extract and rose oil that is effective on the barrier function and hydration of the skin. An emulgel formulation containing 20 % green tea extract and 5 % rose oil was designed as a result of pre-formulation studies. Physicochemical characterization, in vitro stability studies, in vivo water content of the stratum corneum and transepidermal water loss studies were carried out afterwards. In vivo studies on ten female subjects were evaluated by using non-invasive skin bioengineering techniques. Finally, a cosmetically acceptable, stable and effective emulgel formulation for skin barrier function with good hydrating properties was obtained for skin hydration, protection and anti-aging purposes.

Keywords: green tea extract; rose oil; emulgel; skin water content; transepidermal water loss; biophysical measurements

  • 1. A. Sharma, S. Gupta, I. P. Sarethy, S. Dang and R. Gabrani, Green tea extract: possible mechanism and antibacterial activity on skin pathogens, Food Chem. 135 (2012) 672-675; DOI: 10.1016/ j.foodchem.2012.04.143.Web of ScienceCrossrefGoogle Scholar

  • 2. T. Mahmood and N. Akhtar, Combined topical application of lotus and green tea improves facial skin surface parameters, Rejunevation Res. 16 (2013) 91-97; DOI: 10.1089/rej.2012.1380.CrossrefGoogle Scholar

  • 3. S. Hsu, Green tea and skin, J. Am. Acad. Dermatol. 52 (2005) 1049-1059; DOI: 10.1016/j.jaad.2004. 12.044.CrossrefGoogle Scholar

  • 4. C. S. Yang, H. Wang, G. X. Li, Z. Yang, F. Guan and H. Jin, Cancer prevention by tea: evidence from laboratory studies, Pharmacol. Res. 64 (2011) 113-122; DOI: 10.1016/j.phrs.2011.03.001.CrossrefGoogle Scholar

  • 5. T. Mahmood, N. Akhtar, B. A. Khan, H. M. Shoaib Khan and T. Saeed, Changes in skin mechanical properties after long term application of cream containing green tea extract, Aging Clin. Exp. Res. 23 (2011) 333-336.Google Scholar

  • 6. J. I. Silverberg, J. Jagdeo, M. Patel, D. Siegel and N. Brody, Green tea extract protects human skin fibroblasts from reactive oxygen species induced necrosis, J. Drugs Dermatol. 10 (2011) 1096-1101.Google Scholar

  • 7. A. Rasheed, S. N. Shama, J. M. Joy, B. S. Reddy and C. Roja, Formulation and evaluation of herbal anti-acne moisturizer, Pak. J. Pharm. Sci. 25 (2012) 867-870.Google Scholar

  • 8. F. Casetti, U. Wölfle, W. Gehring and C. M. Schempp, Dermocosmetics for dry skin: a new role for botanical extracts, Skin Pharmacol. Physiol. 24 (2011) 289-293; DOI: 10.1159/000329214.Web of ScienceCrossrefPubMedGoogle Scholar

  • 9. S. Ulusoy, G. Bosgelmez-Tinaz and H. Seçilmis-Canbay, Tocopherol, carotene, phenolic contents and antibacterial properties of rose essential oil, hydrosol and absolute, Curr. Microbiol. 59 (2009) 554-558; DOI: 10.1007/s00284-009-9475-y.Web of ScienceCrossrefPubMedGoogle Scholar

  • 10. A. Wei and T. Shibamoto, Antioxidant activities of essential oil mixtures toward skin lipid squalene oxidized by UV irradiation, Cutan. Ocul. Toxicol. 26 (2007) 227-233; DOI: 10.1080/15569520 701224501.Web of ScienceCrossrefGoogle Scholar

  • 11. T. Hongratanaworakit, Relaxing effect of rose oil on humans, Nat. Prod. Commun. 4 (2009) 291-296.Google Scholar

  • 12. A. R. Silva, C. Seidl, A. S. Furusho, M. M. Boeno, G. C. Dieamant and A. M. Weffort-Santos, In vitro evaluation of the efficacy of commercial green tea extracts in UV protection, Int. J. Cosmet. Sci. 35 (2013) 69-77; DOI: 10.1111/ics.12006.CrossrefWeb of ScienceGoogle Scholar

  • 13. P. Kullavanijaya and H. W. Lim, Photoprotection, J. Am. Acad. Dermatol. 52 (2005) 937-958; DOI: 10.1016/j.jaad.2004.07.063.CrossrefGoogle Scholar

  • 14. M. P. Lupo, Antioxidants and vitamins in cosmetics, Clin. Dermatol. 19 (2001) 467-473.CrossrefGoogle Scholar

  • 15. S. E. Dal Belo, L. R. Gaspar, P. M. Maia Campos and J. P. Marty, Skin penetration of epigallocatechin-3-gallate and quercetin from green tea and Ginkgo biloba extracts vehiculated in cosmetic formulations, Skin Pharmacol. Physiol. 22 (2009) 299-304; DOI: 10.1159/000241299.Web of ScienceCrossrefGoogle Scholar

  • 16. P. V. Bhanu, V. Shanmugam and P. K. Lakshmi, Development and optimization of novel diclofenac emulgel for topical drug delivery, Pharmacie Globale 9 (2011) 1-4.Google Scholar

  • 17. A. Sakulpanich and W. Gritsanapan, Extraction method for high content of anthraquinones from cassia fistula pods, J. Health Res. 22 (2008) 167-172.Google Scholar

  • 18. S. Ghosh and D. Rousseau, Freeze-thaw stability of water-in-oil emulsions, J. Colloid. Interface Sci. 339 (2009) 91-102; DOI: 10.1016/j.jcis.2009.07.047.CrossrefWeb of ScienceGoogle Scholar

  • 19. D. Vasiljevic, J. Parojcic, M. Primorac and G. Vuleta, An investigation into the characteristics and drug release properties of multiple W/O/W emulsion systems containing low concentration of lipophilic polymeric emulsifier, Int. J. Pharm. 309 (2006) 171-177; DOI:10.1016/j.ijpharm.2005.11.034.CrossrefGoogle Scholar

  • 20. M. M. Rieger, Emulsions, in Theory and Practice of Industrial Pharmacy (Eds. L. Lachman, H. A. Lieberman and J. L. Kanig), Verlag Lea and Febiger Publishing, Philadelphia 1986, pp. 527-530.Google Scholar

  • 21. J. Pinnagoda, R. A. Tupker, T. Agner and J. Serup, Guidelines for transepidermal water loss (TEWL) measurement, Contact Dermatitis 22 (1990) 164-178; DOI: 10.1111/j.1600-0536.1990.tb01553.xCrossrefGoogle Scholar

  • 22. Y. Cheng, Y. Dong, C. Wang, N. Su, Y. Sun, J. Liu, H. Zheng, A. Schrader, M. Rohr and W. Liu, Protection effect of cosmetics on human skin under simulated rigorous environment, Skin. Res.Technol. 14 (2008) 45-52; DOI: 10.1111/j.1600-0846.2007.00260.x.Web of ScienceCrossrefGoogle Scholar

  • 23. G. Betz, A. Aeppli, N. Menshutina and H. Leuenberger, In vivo comparison of various liposome formulations for cosmetic application, Int. J. Pharm. 296 (2005) 44-54; DOI: 10.1016/j.ijpharm.2005.02.032.CrossrefGoogle Scholar

  • 24. P. Clarys, R. Clijsen, J. Taeymans and A. O. Barel, Hydration measurements of the stratum corneum: comparison between the capacitance method (digital version of the Corneometer CM 825®) and the impedance method (Skicon-200EX®), Skin. Res. Technol. 18 (2012) 316-323; DOI: 10.1111/j.1600-0846.2011.00573.x.CrossrefWeb of ScienceGoogle Scholar

  • 25. J. Du Plessis, A. Stefaniak, F. Eloff, S. John, T. Agner, T. C. Chou, R. Nixon, M. Steiner, A. Franken, I. Kudla and L. Holness, International guidelines for the in vivo assessment of skin properties in non-clinical settings: part 2. Transepidermal water loss and skin hydration, SkinRes.Technol. 1 (2013) 1-10; DOI: 10.1111/srt.12037.CrossrefWeb of ScienceGoogle Scholar

  • 26. P. K. Mukherjee, N. Maity, N. K. Nema and B. K. Sarkar, Bioactive compounds from natural resources against skin aging, Phytomedicine 19 (2011) 64-73; DOI: 10.1016/j.phymed.2011.10.003.CrossrefWeb of ScienceGoogle Scholar

  • 27. R. Darlenski, S. Sassning, N. Tsankov and J. W. Fluhr, Noninvasive in vivo methods for investigation of the skin barrier physical properties, Eur. J. Pharm. Biopharm. 72 (2009) 295-303; DOI: 10.1016/j.ejpb.2008.11.013.CrossrefWeb of ScienceGoogle Scholar

  • 28. B. Sotoodian and H. I. Maibach, Noninvasive test methods for epidermal barrier function, Clin.Dermatol. 30 (2012) 301-310; DOI: 10.1016/j.clindermatol.2011.08.016.CrossrefGoogle Scholar

  • 29. K. A. Walters and M. S. Roberts, Skin Structure, Pharmaceuticals, Cosmetics and the Efficacy of Topically Applied Agents, in Dermatologic, Cosmeceutic and Cosmetic Development, Informa Healthcare, New York 2008, pp. 1-10. Google Scholar

  • 30. M. Breternitz, D. Kowatzki, M. Langenauer and P. Elsner, Placebo controlled, double blind, randomized, prospective study of a glycerol based emollient on eczematous skin in atopic dermatitis: biophysical and clinical evaluation, Skin Phamacol. Physiol. 21 (2008) 39-45; DOI: 10.1159/000111134. Web of ScienceCrossrefGoogle Scholar

About the article

Published Online: 2013-12-31

Published in Print: 2013-12-01

Citation Information: Acta Pharmaceutica, Volume 63, Issue 4, Pages 531–544, ISSN (Online) 1846-9558, ISSN (Print) 1330-0075, DOI: https://doi.org/10.2478/acph-2013-0037.

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