Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Acta Pharmaceutica

The Journal of Croatian Pharmaceutical Society

4 Issues per year

IMPACT FACTOR 2016: 1.288
5-year IMPACT FACTOR: 1.600

CiteScore 2016: 1.55

SCImago Journal Rank (SJR) 2016: 0.353
Source Normalized Impact per Paper (SNIP) 2016: 0.854

Open Access
See all formats and pricing
More options …
Volume 65, Issue 4


Fractionation and evaluation of proteins in roots of Echinacea purpurea (L.) Moench

Gabriele Balciunaite / Jovita Juodsnukyte / Arunas Savickas
  • Department of Social Pharmacy and Drug Technology, Lithuanian University of Health Sciences, Kaunas, Lithuania 4Kaunas Botanical Garden of Vytautas Magnus University Kaunas, Lithuania
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Ona Ragazinskiene / Luka Siatkute / Gitana Zvirblyte / Edita Mistiniene / Nijole Savickiene
Published Online: 2015-12-17 | DOI: https://doi.org/10.1515/acph-2015-0036


Echinacea purpurea (L.) Moench, a member of the Asteraceae family, is a plant rich in flavonoids, essential oils, phenolic compounds, saponins, polysaccharides and glycoproteins. The aim of the study was to evaluate the protein content in dried roots of Echinacea purpurea (L.) Moench after homogenization of roots with liquid nitrogen, extraction in 0.01 mol L-1 phosphate-buffered saline (PBS) and purification followed by fractionation of proteins using gel filtration chromatography. Total concentration of proteins was measured using the Bradford method, and evaluation of the molecular mass of proteins was accomplished by applying the SDS-PAGE gel electrophoresis. The Bradford assay revealed that the highest concentration of proteins in fractions collected after gel filtration chomatography was 4.66–6.07 mg mL-1. Glycoproteins, alkamides and polysaccharides in roots of Echinacea purpurea (L.) Moench are chemical compounds that are responsible for their immunomodulatory properties. However, information about the difference of protein contents in fresh and dried roots of E. purpurea is insufficient.

Keywords: Echinacea purpurea (L.) Moench; proteins; gel filtration chromatography; SDS-PAGE


  • 1. D. C. Hell, J. Barnes, L. Anderson, S. Gibbons and J. D. Phillipson, Echinacea species (Echinacea angustifolia (DC.) Hell., Echinacea pallida (Nutt.) Nutt., Echinacea purpurea (L.) Moench): a review of their chemistry, pharmacology and clinical properties, J. Pharm. Pharmacol. 57 (2005) 929-954; DOI: 10.1211/0022357056127.CrossrefGoogle Scholar

  • 2. Q. Zheng, D. Qiu, X. Liu, L. Zhang, S. Cai and X. Zhang, Antiproliferative effect of Dendrobium catenatum Lindley polypeptides against human liver, gastric and breast cancer cell lines, Food Funct. 5 (2015) 1489-1495; DOI: 10.1039/C5FO00060B.CrossrefWeb of ScienceGoogle Scholar

  • 3. J. J. Dong, F. Wu, X. Ye, C. F. Sun, Y. Y. Tian, M. X. Lu, R. Zhang and Z. H. Chen, β-Defensin in Nile tilapia (Oreochromis niloticus): Sequence, tissue expression, and anti-bacterial activity of synthetic peptides, Gene 566 (2015) 23-31; DOI: 10.1016/j.gene.2015.04.025.CrossrefWeb of ScienceGoogle Scholar

  • 4. I. Arcan and A. Yemenicioglu, Antioxidant activity of protein extracts from heat-treated or thermally processed chickpeas and white beans, Food Chem. 103 (2007) 301-312; DOI: 10.1016/j.foodchem. 2006.07.050.CrossrefWeb of ScienceGoogle Scholar

  • 5. P. Abreu, S. Matthew, T. Gonzalez, D. Costa, M. A. Segundo and E. Fernandes, Anti-inflammatory and antioxidant activity of a medicinal tincture from Pedilanthus tithymaloides, Life Sci. 78 (2006) 1578-1585; DOI: 10.1016/j.lfs.2005.07.037.CrossrefGoogle Scholar

  • 6. R. J. Simpson, Protocol: Bulk precipitation of proteins by ammonium sulfate, Cold Spring Harb. Protoc. 2006; DOI: 10.1101/pdb.prot4308.CrossrefGoogle Scholar

  • 7. A. Gupta and R. S. Sandhu, A new high molecular weight agglutinin from garlic (Allium sativum), Molec. Cell. Cell. Bioch. 166 (1997) 1-9; DOI: 10.1023/A:1006827921151.CrossrefGoogle Scholar

  • 8. H. C. Silva, A. U. Bari, B. A. Rocha, K. S. Nascimento, E. L. Ponte, A. F. Pires, P. Delatorre, E. H. Teixeira, H. Debray, A. M. Assreuy, C. S. Nagano and B. S. Cavada, Purification and primary structure of a mannose/glucose-binding lectin from Parkia biglobosa Jacq. seeds with antinociceptive and anti-inflammatory properties, J. Mol. Recogn. 26 (2013) 470-478; DOI: 10.1002/jmr.2289.CrossrefGoogle Scholar

  • 9. M. M. Bradford, A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Anal. Biochem. 72 (1976) 248-254; DOI: 10.1016/0003-2697(76)90527-3.CrossrefGoogle Scholar

  • 10. K. Hyung-Keun, L. Hyuk-Min, O. Kyong-Hwa, Y. Hyo-Jin, J. Ji-Seon and K. Sook-Kyung, Interpretation of protein quantitation using the Bradford assay: Comparison with two calculation models, Anal. Biochem. 434 (2013) 178-180; DOI: 10.1016/j.ab.2012.10.045.CrossrefWeb of ScienceGoogle Scholar

  • 11. S. Thude and B. Classen, High molecular weight constituents from roots of Echinacea pallida: An arabinogalactan-protein and an arabinan, Phytochemistry 66 (2005) 1026-1032; DOI: 10.1016/j.phytochem. 2005.02.028CrossrefGoogle Scholar

  • 12. J. B. Harborne and C. A. Williams, Phytochemistry of the Genus Echinacea, in Echinacea: The genus Echinacea (Ed. S. C. Miller), CRC Press, Boca Raton 2004, pp. 61-77.Google Scholar

  • 13. R. B. Volk, W. Blaschek and B. Classen, Characterization of an arabinogalactan protein from the pressed juice of Echinacea purpurea: investigations into the type of linkage between the protein and polysaccharide moieties, J. Nat. Med. 61 (2007) 397-401; DOI: 10.1007/s11418-007-0174-3.CrossrefWeb of ScienceGoogle Scholar

  • 14. A. Bossy, W. Blaschek and B. Classen, Characterization and immunolocalization of arabinogalactan- proteins in roots of Echinacea purpurea, Planta Med. 75 (2009) 1526-1533; DOI: 10.1055/ s-0029-1185801.CrossrefWeb of ScienceGoogle Scholar

About the article

Accepted: 2015-07-14

Published Online: 2015-12-17

Published in Print: 2015-12-01

Citation Information: Acta Pharmaceutica, Volume 65, Issue 4, Pages 473–479, ISSN (Online) 1846-9558, DOI: https://doi.org/10.1515/acph-2015-0036.

Export Citation

© by Nijole Savickiene. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License. BY-NC-ND 4.0

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

Rayn Clarenc Aarland, Angel Ernesto Bañuelos-Hernández, Mabel Fragoso-Serrano, Edgar del Carmen Sierra-Palacios, Fernando Díaz de León-Sánchez, Laura Josefina Pérez-Flores, Fernando Rivera-Cabrera, and José Alberto Mendoza-Espinoza
Pharmaceutical Biology, 2017, Volume 55, Number 1, Page 649

Comments (0)

Please log in or register to comment.
Log in