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Acta Chimica Slovaca

The Journal of Slovak University of Technology in Bratislava

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1337-978X
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Simulated moving bed chromatography separation of galacto-oligosaccharides

Łukasz Wiśniewski
  • Department of Chemical and Biochemical Engineering, Institute of Chemical and Environmental Engineering, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, 812 37 Bratislava, Slovakia
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/ Monika Antošová
  • Department of Chemical and Biochemical Engineering, Institute of Chemical and Environmental Engineering, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, 812 37 Bratislava, Slovakia
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Milan Polakovič
  • Corresponding author
  • Department of Chemical and Biochemical Engineering, Institute of Chemical and Environmental Engineering, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, 812 37 Bratislava, Slovakia
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Published Online: 2013-11-23 | DOI: https://doi.org/10.2478/acs-2013-0033

Abstract

A mixture of galacto-oligosaccharides and non-prebiotic sugars was separated using a simulated moving bed unit equipped with eight packed-bed columns of a cation exchanger. In order to determine operating conditions using triangle theory, equilibrium adsorption isotherms of galactose, glucose, lactose, tri- and tetragalacto-oligosaccharide were measured. It was found that each column of the SMB unit had a maximum separation efficiency of more than 3000 theoretical plates. The achieved purity of galacto-oligosaccharides in the raffinate stream was 99.9 %, similarly 99.9 % of extract was formed by non-prebiotic sugars lactose, glucose and galactose. The productivities in the raffinate and extract, were 0.6 g/(L h) and 2.4 g/(L h), respectively.

Keywords: simulated moving bed chromatography; galacto-oligosaccharides; adsorption isotherm

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About the article

Published Online: 2013-11-23

Published in Print: 2013-10-01


Citation Information: Acta Chimica Slovaca, ISSN (Print) 1337-978X, DOI: https://doi.org/10.2478/acs-2013-0033.

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