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Acta Veterinaria

The Journal of University of Belgrade

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A survey of deformed wing virus and acute bee paralysis virus in honey bee colonies from serbia using real-time RT-PCR

Predrag Simeunović
  • Corresponding author
  • Department of Biology, Faculty of Veterinary Medicine, University of Belgrade, Bul. Oslobodjenja 18, Belgrade, Serbia;
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  • Other articles by this author:
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/ Jevrosima Stevanović
  • Department of Biology, Faculty of Veterinary Medicine, University of Belgrade, Bul. Oslobodjenja 18, Belgrade, Serbia;
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/ Dejan Vidanović / Jakov Nišavić
  • Department of Microbiology, Faculty of Veterinary Medicine, University of Belgrade, Bul. Oslobodjenja 18, Belgrade, Serbia;
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  • De Gruyter OnlineGoogle Scholar
/ Dejan Radović / Ljubodrag Stanišić
  • Department of Biology, Faculty of Veterinary Medicine, University of Belgrade, Bul. Oslobodjenja 18, Belgrade, Serbia;
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  • De Gruyter OnlineGoogle Scholar
/ Zoran Stanimirović
  • Department of Biology, Faculty of Veterinary Medicine, University of Belgrade, Bul. Oslobodjenja 18, Belgrade, Serbia;
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  • De Gruyter OnlineGoogle Scholar
Published Online: 2014-03-25 | DOI: https://doi.org/10.2478/acve-2014-0009


In this study 55 honey bee colonies from different Serbian regions were monitored for the presence of Deformed Wing Virus (DWV) and Acute Bee Paralysis Virus (ABPV) using TaqMan-based real-time RT-PCR assay. The results revealed the presence of DWV in each sampling location, and ABPV in 10 out of 11 apiaries. High frequency of DWV (76.4%) and ABPV (61.8%) positive samples in asymptomatic colonies can be the consequence of inefficient and postponed Varroa treatment concerning the role of this mite in the transmission and activation of honey bee viruses. The real-time RTPCR technique described in this paper is proved to be the most reliable method for this kind of investigation.

Keywords: DWV; ABPV; Varroa destructor; Serbian apiaries


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About the article

Published Online: 2014-03-25

Published in Print: 2014-03-01

Citation Information: Acta Veterinaria, Volume 64, Issue 1, Pages 81–92, ISSN (Online) 1820-7448, DOI: https://doi.org/10.2478/acve-2014-0009.

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