Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Acta Veterinaria

The Journal of University of Belgrade

4 Issues per year


CiteScore 2016: 0.65

SCImago Journal Rank (SJR) 2016: 0.388
Source Normalized Impact per Paper (SNIP) 2016: 0.605

Open Access
Online
ISSN
1820-7448
See all formats and pricing
More options …

Immuno-histomorphometric and -fluorescent characteristics of GH cells after treatment with genistein or daidzein in an animal model of andropause

Vladimir Ajdžanović
  • Corresponding author
  • Department of Cytology, Institute for Biological Research “Siniša Stanković”, University of Belgrade, Belgrade, Serbia;
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Ivana Medigović
  • Department of Cytology, Institute for Biological Research “Siniša Stanković”, University of Belgrade, Belgrade, Serbia;
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Jasmina Živanović
  • Department of Cytology, Institute for Biological Research “Siniša Stanković”, University of Belgrade, Belgrade, Serbia;
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Branka Šošić-Jurjević
  • Department of Cytology, Institute for Biological Research “Siniša Stanković”, University of Belgrade, Belgrade, Serbia;
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Svetlana Trifunović
  • Department of Cytology, Institute for Biological Research “Siniša Stanković”, University of Belgrade, Belgrade, Serbia
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Nasta Tanić
  • Department of Radiobiology and Molecular Genetics, Institute of Nuclear Sciences “Vinča”, University of Belgrade, Belgrade, Serbia
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Verica Milošević
  • Department of Cytology, Institute for Biological Research “Siniša Stanković”, University of Belgrade, Belgrade, Serbia;
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2014-03-25 | DOI: https://doi.org/10.2478/acve-2014-0010

Abstract

Somatopause, the complex aspect of andropause, is recognizable by reduced growth hormone - GH/insulin-like growth factor 1 axis function in the ageing male. Soy isoflavones (usually genistein and daidzein), which are known for their beneficial effects in the treatment of ageing symptoms, are active in the pituitary, as well. The immunohistomorphometric and -fluorescent characteristics of pituitary growth hormone secreting cells, in an animal model of andropause, were examined after a treatment with genistein or daidzein. Andropausal Wistar rats were divided into sham operated, orchidectomized and genistein or daidzein treated orchidectomized groups. Genistein or daidzein (30 mg/kg/day) were administered subcutaneously for three weeks, while sham operated and orchidectomized groups received the vehicle alone. Growth hormone secreting cells were identified by the peroxidase-antiperoxidase immuno-histochemical, and immuno-fluorescent procedure. The main characteristic of growth hormone secreting cells in soy isoflavones treated groups is a weaker immuno-histochemical staining and immuno-fluorescent signal compared to sham operated and orchidectomized groups. The growth hormone secreting cell volume in orchidectomized +genistein or +daidzein groups is by 13.8% and 11.9% (p<0.05) smaller respectively, in comparison with the orchidectomized group. In orchidectomized +genistein or +daidzein groups, the growth hormone secreting cells relative volume density is by 62.5% and 61.0% lower (p<0.05) respectively than for the sham operated group, and decreased by 65.4% and 64.0% (p<0.05) respectively, compared to the orchidectomized group. It can be concluded that chronic genistein or daidzein treatment, in an animal model of andropause, attenuates immunohistomorphometric and -fluorescent characteristics of growth hormone secreting cells.

Keywords : andropause; GH cells; immuno-fluorescence; immuno-histochemistry; soy isoflavones

References

  • 1. Melmed S: Idiopathic adult growth hormone defi ciency. J Clin Endocrinol Metab 2013, 98:2187-2197.CrossrefGoogle Scholar

  • 2. Morales A: Andropause (or s ymptomatic late-onset hypogonadism): facts, fi ction and controversies. Aging Male 2004, 7:297-303.CrossrefGoogle Scholar

  • 3. Thompson CA, Shanafelt TD, Loprinzi CL: Andropause: symptom management for prostate cancer patients treated with hormonal ablation. Oncologist 2003, 8:474-487.PubMedCrossrefGoogle Scholar

  • 4. Epelbaum J: Neuroendocrinol ogy and aging. J Neuroendocrinol 2008, 20:808-811.CrossrefGoogle Scholar

  • 5. Milosevic V, Trifunovic S, Sosic-Jurjevic B, Bujsic N, Sekulic M: Estradiol and calcium affect the growth hormone producing cells in female middle-aged rats. Acta Vet-Beograd 2005, 55:111-120.Google Scholar

  • 6. Russell-Aulet M, Dimaraki E V, Jaffe CA, DeMott-Friberg R, Barkan AL: Aging-related growth hormone (GH) decrease is a selective hypothalamic GH-releasing hormone pulse amplitude mediated phenomenon. J Gerontol A Biol Sci Med Sci 2001, 56:M124-129.CrossrefGoogle Scholar

  • 7. Kuwahara S, Sari DK, Tsukam oto Y, Tanaka S, Sasaki F: Age-related changes in growth hormone (GH) cells in the pituitary gland of male mice are mediated by GH-releasing hormone but not by somatostatin in the hypothalamus. Brain Res 2004, 998:164-173.Google Scholar

  • 8. Takahashi S: Heterogenity a nd development of somatotrophs and mammotrophs in the rat. Zoolog Sci 1992, 9:901-924.Google Scholar

  • 9. Ceda GP, Dall’Aglio E, Morg anti S, Denti L, Maggio M, Lauretani F, Andrea A, Ceresini G, Cattabiani C, Valenti G: Update on new therapeutic options for the somatopause. Acta Biomed 2010, 81 Suppl 1:67-72.Google Scholar

  • 10. Misztal T, Wankowska M, Go rski K, Romanowicz K: Central estrogen-like effect of genistein on growth hormone secretion in the ewe. Acta Neurobiol Exp (Wars) 2007, 67:411-419.Google Scholar

  • 11. Scanlan N, Skinner DC: Est radiol modulation of growth hormone secretion in the ewe: no growth hormone-releasing hormone neurons and few somatotropes express estradiol receptor alpha. Biol Reprod 2002, 66:1267-1273.CrossrefGoogle Scholar

  • 12. Ogiwara T, Chik CL, Ho AK: Tyrosine kinase inhibitors enhance GHRH-stimulated cAMP accumulation and GH release in rat anterior pituitary cells. J Endocrinol 1997, 152:193-199. Google Scholar

  • 13. Picherit C, Coxam V, Benne tau-Pelissero C, Kati-Coulibaly S, Davicco MJ, Lebecque P, Barlet JP: Daidzein is more effi cient than genistein in preventing ovariectomy-induced bone loss in rats. J Nutr 2000, 130:1675-1681.Google Scholar

  • 14. Ajdzanovic VZ, Sosic-Jurje vic BT, Filipovic BR, Trifunovic SL, Milosevic V: Daidzein effects on ACTH cells: immunohistomorphometric and hormonal study in an animal model of the andropause. Histol Histopathol 2011, 26:1257-1264.Google Scholar

  • 15. Ajdzanovic VZ, Sosic-Jurje vic BT, Filipovic BR, Trifunovic SL, Brkic DD, Sekulic MI, Milosevic V: Genistein affects the morphology of pituitary ACTH cells and decreases circulating levels of ACTH and corticosterone in middle-aged male rats. Biol Res 2009, 42:13-23.Google Scholar

  • 16. Pantelic J, Filipovic B, S osic-Jurjevic B, Ajdzanovic V, Trifunovic S, Medigovic I, Milosevic V: Vitex agnus-castus essential oil affects thyroid C cells and bone metabolism in middleaged male rats. Acta Vet-Beograd 2013, 63:23-35.Web of ScienceCrossrefGoogle Scholar

  • 17. Sternberger LA, Hardy PH, Jr., Cuculis JJ, Meyer HG: The unlabeled antibody enzyme method of immunohistochemistry: preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-antihorseradish peroxidase) and its use in identifi cation of spirochetes. J Histochem Cytochem 1970, 18:315-333.CrossrefGoogle Scholar

  • 18. Milosevic V, Brkic B, Velk ovski S, Starcevic V: Somatostatin induced changes of the ACTH and STH cells in the hypophysis of the male rats. Jugoslav Med Biohem 1994, 13:102-106.Google Scholar

  • 19. Weibel ER: 1. Practical Me thods for Biological Morphometry. In: Stereological Methods. London, UK: Academic Press; 1979, 1-415.Google Scholar

  • 20. Poole MC, Kornegay 3rd WD: Cellular distribution within the rat adenohypophysis: a morphometric study. Anat Rec 1982, 204:45-53.Google Scholar

  • 21. Filipovic B, Sosic-Jurjevi c B, Ajdzanovic V, Brkic D, Manojlovic-Stojanoski M, Milosevic V, Sekulic M: Daidzein administration positively affects thyroid C cells and bone structure in orchidectomized middle-aged rats. Osteoporos Int 2010, 21:1609-1616.PubMedCrossrefGoogle Scholar

  • 22. Penza M, Montani C, Romani A, Vignolini P, Pampaloni B, Tanini A, Brandi ML, Alonso- Magdalena P, Nadal A, Ottobrini L et al: Genistein affects adipose tissue deposition in a dose-dependent and gender-specifi c manner. Endocrinology 2006, 147:5740-5751.Google Scholar

  • 23. Faqi AS, Johnson WD, Morri ssey RL, McCormick DL: Reproductive toxicity assessment of chronic dietary exposure to soy isofl avones in male rats. Reprod Toxicol 2004, 18:605-611.CrossrefGoogle Scholar

  • 24. Yokoshiki H, Sumii K, Sper elakis N: Inhibition of L-type calcium current in rat ventricular cells by the tyrosine kinase inhibitor, genistein and its inactive analog, daidzein. J Mol Cell Cardiol 1996, 28:807-814.CrossrefGoogle Scholar

  • 25. Zhong W, Atkin SL: Modulat ion of somatostatin receptors expression by genistein in human umbilical vein endothelial cells. Endocrine Abstracts 2005, 9:P38.Google Scholar

  • 26. Barbieri F, Bajetto A, Pat tarozzi A, Gatti M, Wurth R, Thellung S, Corsaro A, Villa V, Nizzari M, Florio T: Peptide receptor targeting in cancer: the somatostatin paradigm. Int J Pept 2013, 2013:926295. Google Scholar

About the article

Published Online: 2014-03-25

Published in Print: 2014-03-01


Citation Information: Acta Veterinaria, Volume 64, Issue 1, Pages 93–104, ISSN (Online) 1820-7448, DOI: https://doi.org/10.2478/acve-2014-0010.

Export Citation

This content is open access.

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

[1]
Svetlana Trifunović and Verica Milošević
Macedonian Veterinary Review, 2017, Volume 0, Number 0

Comments (0)

Please log in or register to comment.
Log in