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Acta Veterinaria

The Journal of University of Belgrade

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Online
ISSN
1820-7448
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Typing of Indigenous Campylobacter spp. From Serbia by M-PCR and RAPD

Dragana Jošić
  • Institute for Soil Science, Teodora Drajzera 7, 11 000 Belgrade, Serbia
/ Jelena Petković
  • Corresponding author
  • Institute of Veterinary Medicine, Boška Jovića 6, 35 000 Jagodina, Serbia
  • Email:
/ Olivera Bunčić
  • Faculty of Veterinary Medicine, Bulevar Oslobođenja 18, 11 000 Belgrade, Serbia
/ Zorica Lepšanović
  • Military Medical Academy, Crnotravska 17, 11000 Belgrade, Serbia
/ Radmila Pivić
  • Institute for Soil Science, Teodora Drajzera 7, 11 000 Belgrade, Serbia
/ Zoran Rašić
  • Institute of Veterinary Medicine, Boška Jovića 6, 35 000 Jagodina, Serbia
/ Vera Katić
  • Faculty of Veterinary Medicine, Bulevar Oslobođenja 18, 11 000 Belgrade, Serbia
Published Online: 2016-06-28 | DOI: https://doi.org/10.1515/acve-2016-0017

Abstract

Campylobacteriosis is an infectious human disease caused by thermophilic Campylobacter species, mainly C. jejuni and C. coli. It is the leading cause of human gastroenteritis today with the number of cases surpassing the number of Salmonella poisoning cases. The epidemiology of the agent is not completely clear, but a number of investigations indicate an important role of broiler meat in human infections. The purpose of this study was to investigate the diversity of Campylobacter jejuni strains present in the Republic of Serbia and to determine a fast and reliable system for the confirmation and typing of the isolated strains. Samples taken at slaughterhouses, broiler farms, as well as two human isolates of Campylobacter species have been investigated. Strain identification was performed by multiplex-PCR. Genotyping was performed by Random Amplified Polymorphic DNA (RAPD) with multiple primers. Using several unusual primers and a newly designed one (DJP17), we report on the RAPD types of indigenous Campylobacter species. RAPD profiles showed different levels of discrimination between the isolates, depending on the primer: SPH1 and AG15 were informative only in part and better results were obtained with AP10, AK16 and DJP17.

Keywords: broilers; Campylobacter spp.; epidemiology; multiplex-PCR; RAPD

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About the article

Received: 2015-10-15

Accepted: 2016-04-06

Published Online: 2016-06-28

Published in Print: 2016-06-01


Citation Information: Acta Veterinaria, ISSN (Online) 1820-7448, DOI: https://doi.org/10.1515/acve-2016-0017. Export Citation

© by Jelena Petković. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License. (CC BY-NC-ND 4.0)

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