Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Acta Veterinaria

The Journal of University of Belgrade

4 Issues per year

CiteScore 2016: 0.65

SCImago Journal Rank (SJR) 2016: 0.388
Source Normalized Impact per Paper (SNIP) 2016: 0.605

Open Access
See all formats and pricing
More options …

Scanning Electron Microscopy Analysis of Changes of Hydroxiapatite/Poly-L-Lactide with Different Molecular Weight of PLLAaAfter Intraperitoneal Implantation

Ljubiša Đorđević
  • Corresponding author
  • Department of Biology with Ecology, Faculty of Science and Mathematics, University of Niš, Serbia
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Stevo Najman / Perica Vasiljević
  • Department of Biology with Ecology, Faculty of Science and Mathematics, University of Niš, Serbia
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Miroslav Miljković / Nenad Ignjatović / Dragan Uskoković / Milenko Plavšić
Published Online: 2016-06-28 | DOI: https://doi.org/10.1515/acve-2016-0020


Implantation of a biomaterial is one of the important trends in solving the problem of bone tissue loss. Calcium hydroxiapatite (HAp), as the most representative bone component is a serious candidate for such implantations. The synthetic polymer poly-L-lactide (PLLA) in HAp/PLLA is often used as a polymeric material, with a role in the substitution of bone tissue collagen fibers. Fibers of PLLA may strengthen HAp and its good bioresorption provides space for tissue remodeling. Differences in porosity, microstructure, compressive consistency as well as bioresorbility of HAp/ PLLA may be achieved by using PLLA with different molecular weights. In this study HAp/PLLA composites with PLLA of different molecular weights (50,000; 160,000 and 430,000) were implanted in mouse peritoneum in order to examine the influence of the molecular weight of PLLA on morphology changes. Microstructural changes of biomaterial (HAp/PLLA) surface were analyzed one week, three weeks and four months after their implantation using Scanning Electron Microscopy. The results showed a significant difference in tissue reactions on the applied biocomposites, depending on their molecular weight. The most intense proliferation of cells was induced by HAp/PLLA 50,000 compared to HAp/PLLA 430,000 and HAp/PLLA 160,000. In the vicinity of HAp/PLLA 430,000 abundant erythrocytes were observed. The differences in biological reactions on the examined biocomposites are significant for their practical applications. HAp/PLLA composite biomaterials of different types and resorption rates require specific designing and programming to become suitable for particular purposes in an organism.

Keywords: calcium hydroxiapatite; poly-L-lactide; implant; mouse; peritoneum; collagen; SEM


  • 1. Keating FJ, McQueen MM. Substitutes for autologous bone graft in orthopaedic trauma. J Bone Joint Surg 2001; 83(1): 3-8.CrossrefGoogle Scholar

  • 2. Lazić Z, Bubalo M, Milović R, Matijević S, Magić M, Đorđević I. Comparison of resorbable membranes for guided bone regeneration of human and bovine origin. Acta Veterinaria- Beograd 2014; 64(4): 477-492.Web of ScienceGoogle Scholar

  • 3. Gao C, Deng Y, Feng P, Mao Z, Li P, Yang B, Deng J, Cao Y, Shuai C, Peng S. Current progress in bioactive ceramic scaffolds for bone repair and regeneration. Int J Mol Sci 2014; 15: 4714-4732.CrossrefWeb of ScienceGoogle Scholar

  • 4. Kurashina K, Kurita H, Takeuchi H, Hirano M, Klein C, de-Groot K. Osteogenesis in muscle with composite graft of hydroxyapatite and autogenous calvarial periosteum: A preliminary report. Biomaterials 1995; 16(2): 119-123.CrossrefGoogle Scholar

  • 5. Ripamonti U, Duneas N. Tissue engineering of bone by osteoinductive biomaterials. MRS Bulletin 1996; 21(11): 36-42.Google Scholar

  • 6. Angelova N, Hunkeler D. Rationalizing the design of polymeric biomaterials. Trends in Biotechnology 1999; 17(10): 409-421.CrossrefGoogle Scholar

  • 7. Wang Z, Wang Y, Ito Y, Zhang P, Chen X. A comparative study on the in vivo degradation of poly(L-lactide) based composite implants for bone fracture fixation, Scientific Reports 2016; 9;6:20770Web of ScienceGoogle Scholar

  • 8. Yanagida H, Okada M,Masuda M, Narama I, Nakano S, Kitao S, Takakuda K, Furuzono T. Preparation and in vitro/in vivo evaluations of dimpled poly(l-lactic acid) fi bers mixed/ coated with hydroxyapatite nanocrystals. Journal of Artificial Organs 2011; 14, 331-341.Web of ScienceCrossrefGoogle Scholar

  • 9. Freed L E, Vunjak-Novakovic G, Biron R J, Eagles D, Lesnoy D, Barlow S K, Langer R. Biodegradable polymer scaffolds for tissue engineering. Nature Biotechnology 1994; 12: 689-693.CrossrefGoogle Scholar

  • 10. Ignjatović N, Tomić S, Dakić M, Miljković M, Plavšić M, Uskoković D. Synthesis and properties of hydroxyapatite/poly-L-lactide composite biomaterials. Biomaterials 1999; 20(9): 809-816. CrossrefGoogle Scholar

  • 11. Nejati E, Firouzdor V, Eslaminejad M B, Bagheri F. Needle-like nano hydroxyapatite/ poly(L-lactide acid) composite scaffold for bone tissue engineering application. Materials Science and Engineering C 2009; 29: 942-949.CrossrefGoogle Scholar

  • 12. Ignjatović N, Savić V, Najman S, Plavšić M, Uskoković D. A study of HAp/PLLA composite as a substitute for bone powder, using FT-IR spectroscopy. Biomaterials 2001; 22(6): 571-575.CrossrefGoogle Scholar

  • 13. Najman S, Đorđević Lj, Savić V, Ignjatović N, Plavšić M, Uskoković D. Changes of HAp/PLLA biocomposites and tissue reaction after subcutaneous implantation. Facta Universitatis Series: Medicine and Biology 2003; 10(3): 131-134.Google Scholar

  • 14. Najman S, Savic V, Djordjevic Lj. Ignjatovic N, Uskokovic D. Biological evaluation of hydroxyapatite/poly-L-lactide (HAp/PLLA) composite biomaterials with poly-L-lactide of different molecular weights intraperitoneally implanted into mice. Biomed Mater Eng 2004; 14(1): 61-70.Google Scholar

  • 15. Persson M, Lorite SG, Kokkonen EH, Cho SW, Lehenkari PP, Skrifvars M, Tuukkanen J. Effect of bioactive extruded PLA/HA composite films on focal adhesion formation of preosteoblastic cells. Colloids and Surfaces B: Biointerfaces 2014; 121: 409-416.Web of ScienceCrossrefGoogle Scholar

  • 16. Mansourizadeh F, Asadi A, Oryan S, Nematollahzadeh A, Dodel M, Asghari-Vostakolaei M. PLLA/HA Nano composite scaffolds for stem cell proliferation and differentiation in tissue engineering. Molecular Biology Research Communications 2013; 2(1-2): 1-10.Google Scholar

  • 17. Mainil-Varlet P, Curtis R, Gogolewski S. Effect of in vivo and in vitro degradation on molecular and mechanical properties of various low-molecular-weight polylactides. J Biomed Mater Res 1997; 36(3): 360-80.CrossrefGoogle Scholar

  • 18. Podlaha J, Schwanhaeuser K. Experimental assessment of a new type of vascular prostheses with adiponectin (adipograft Ra 1vk 7/350) on sheep. Acta Veterinaria-Beograd 2014; 64(4): 426-437. Web of ScienceGoogle Scholar

About the article

Received: 2016-03-18

Accepted: 2016-05-24

Published Online: 2016-06-28

Published in Print: 2016-06-01

Citation Information: Acta Veterinaria, ISSN (Online) 1820-7448, DOI: https://doi.org/10.1515/acve-2016-0020.

Export Citation

© by Ljubiša Đorđević. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License. BY-NC-ND 4.0

Comments (0)

Please log in or register to comment.
Log in