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European Pharmaceutical Journal

Acta Facultatis Pharmaceuticae Universitatis Comenianae (formerly)

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Antimicrobial susceptibility and immunomodulatory properties of lamb isolate of lactobacillus mucosae, new probiotic candidate

Andrea Bilková
  • Corresponding author
  • Comenius University in Bratislava, Faculty of Pharmacy, Department of Cell and Molecular Biology of Drugs, Bratislava, Slovak Republic
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  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Martina Dubničková
  • Comenius University in Bratislava, Faculty of Pharmacy, Department of Cell and Molecular Biology of Drugs, Bratislava, Slovak Republic
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Hana Kiňová Sepová
  • Comenius University in Bratislava, Faculty of Pharmacy, Department of Cell and Molecular Biology of Drugs, Bratislava, Slovak Republic
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2013-12-31 | DOI: https://doi.org/10.2478/afpuc-2013-0017


In the process of selecting a new probiotic candidate, several bacteria were isolated from the stomach mucosa of a lamb. Among them, three lactobacilli strains were identified and partially characterised. The strain, Lactobacillus mucosae D, showed several characteristics appropriate to the probiotics. In this study, we have focused on the further characterisation of L. mucosae D and testing of its ability to modulate metabolic and immunomodulatory activities of human mononuclear cells in vitro. L. mucosae D is resistant to antibiotics, like penicillin G, oxacillin, vancomycin and chemotherapeutics ofloxacin and ciprofloxacin. In in vitro conditions, L. mucosae D caused a significant increase in phagocytic activity and index (relative activities 1.05 and 1.44, respectively) of human monocytes. It decreased bactericidal activities of monocytes against Escherichia coli (relative activity 0.73) and Staphylococcus aureus (relative activity 0.36), whereas, candidacidal activity was enhanced (relative activity 1.15). Metabolic activities, lysozyme and peroxidase activity, of mononuclear cells were not changed or increased, respectively. L. mucosae D displayed the ability to enhance production of pro-inflammatory cytokine, IL-1β, in monocytes in vitro (relative activity 2.60). Therefore, we state that lamb isolate, L. mucosae D, has the required attributes for being a potential probiotic candidate.

Slovak abstract

S cieľom ziskať novych probiotickych kandidatov, bolo zo žaludočnej sliznice jahňaťa izolovanych niekoľko bakterii. Tri z nich boli identifikovane ako laktobacily a boli čiastočne charakterizovane. Kmeň Lactobacillus mucosae D preukazal v predošlych experimentoch niekoľko vlastnosti typickych pre probiotika. V tejto praci sme sa zamerali na ďalšiu charakterizaciu Lactobacillus mucosae Da sledovanie jeho schopnosti modulovať metabolicke a imunomodulačne vlastnosti ľudskych mononukleovych buniek v podmienkach in vitro. Zistili sme, že L. mucosae D je rezistentny voči nasledujucim antibiotikam: penicilin G, oxacilin, vankomycin a chemoterapeutikam ofloxacin a ciprofloxacin. V podmienkach in vitro L. mucosae D sposobil signifikantne zvyšenie fagocytovej aktivity a indexu (relativne aktivity 1,05 a 1,44) ľudskych monocytov. Baktericidnu aktivitu monocytov voči Escherichia coli znižil (relativna aktivita 0,73) rovnako ako aktivitu voči Staphylococcus aureus (relativna aktivita 0,36), zatiaľ čo kandidacidnu aktivitu zvyšil (relativna aktivita 1,15). Z metabolickych aktivit mononukleovych buniek sme u lyzozymovej aktivity nepozorovali žiadnu zmenu, naopak peroxidazova aktivita bola zvyšena. L. mucosae D preukazal schopnosť potencovať produkciu prozapaloveho cytokinu IL-1β monocytmi v podmienkach in vitro (relativna aktivita 2,60). Na zaklade ziskanych vysledkov považujeme jahňaci izolat L. mucosae D za potencialne probioticky.

Keywords: Lactobacillus mucosae; probiotics; immunomodulation

Klúcové slová : Lactobacillus mucosae; probiotiká; imunomodulácia

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About the article

Published Online: 2013-12-31

Published in Print: 2013-12-01

Citation Information: Acta Facultatis Pharmaceuticae Universitatis Comenianae, Volume 60, Issue 2, Pages 1–6, ISSN (Online) 1338-6786, ISSN (Print) 0301-2298, DOI: https://doi.org/10.2478/afpuc-2013-0017.

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