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European Pharmaceutical Journal

Acta Facultatis Pharmaceuticae Universitatis Comenianae (formerly)

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CiteScore 2016: 0.28

SCImago Journal Rank (SJR) 2015: 0.195
Source Normalized Impact per Paper (SNIP) 2015: 0.202

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2453-6725
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Elicitation – a tool to improve secondary metabolites production in Melissa Officinalis L. Suspension cultures / Elicitácia ako nástroj na zlepšenie produkcie sekundárnych metabolitov v suspenzných kultúrach Melissa Officinalis L.

J. Urdová
  • Comenius University in Bratislava, Faculty of Pharmacy, Department of Pharmacognosy and Botany, Slovak Republic
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/ M. Rexová
  • Comenius University in Bratislava, Faculty of Pharmacy, Department of Pharmacology and Toxicology, Slovak Republic
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/ P. Mučaji
  • Comenius University in Bratislava, Faculty of Pharmacy, Department of Pharmacognosy and Botany, Slovak Republic
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/ A. Balažová
  • Corresponding author
  • Comenius University in Bratislava, Faculty of Pharmacy, Department of Cell and Molecular Biology of Drugs, Slovak Republic
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Published Online: 2015-06-29 | DOI: https://doi.org/10.1515/afpuc-2015-0012

Abstract

Our recent study is focussed on the investigation of the influence of biotic (Botrytis cinerea, methyl jasmonate) and abiotic (cadmium and cobalt chloride) elicitors on Melissa officinalis L. (lemon balm) suspension cultures production properties. The plant material was treated with different concentrations of methyl jasmonate (10 and 100 μmol.l−1), with a phytopathogenic fungus hydrolyzate (Botrytis cinerea, 1 and 2 ml with glucose equivalent 24 μg.ml−1), cadmium and cobalt (II) chloride (both at concentrations 100 μmol.l−1 and 1 μmol.l−1). Elicited suspension cultures were harvested after 24, 48 and 72 h of elicitor treatment. The elicitation effect was evaluated based on hydroxycinnamic acid derivatives content expressed as rosmarinic acid in dose- and time-dependent manners. The Botrytis cinerea hydrolyzate exhibited the best effect on Melissa officinalis L. suspension cultures production properties among biotic elicitors (glucose equivalent 24 mg.ml−1, 1 ml, after 72 h of treatment). The hydroxycinnamic acid derivatives content increased in triplicate compared to non-elicited samples. Methyl jasmonate affected the suspension cultures production properties moderately at concentration 10 μmol.l−1. Chlorides salts of cobalt and cadmium stimulated phenolic compounds production effectively at concentrations 100 μmol.l−1. The presence of cobalt and cadmium ions in suspension cultures growth media increased the hydroxycinnamic acid derivatives content twofold and quadruple, respectively.

Slovak abstract

Súčasná práca je zameraná na štúdium vplyvu biotických (Botrytis cinerea, metyljazmonát) a abiotických elicitorov (chlorid kademnatý a chlorid kobaltnatý) na produkčné vlastnosti suspenzných kultúr medovky lekárskej (Melissa officinalis L.). Rastlinný materiál bol elicitovaný metyljazmonátom (10 and 100 μmol.l-1), hydrolyzátom bunkovej steny fytopatogénnej huby Botrytis cinerea (1 a 2 ml s glukózovým ekvivalentom 24μg.ml-1), chloridom kademnatým a kobaltnatým (oba v koncentráciách 100 and 1 μmol.l-1). Elicitované kultúry boli vystavené expozícii elicitorov po dobu 24, 48 a 72 h. Efekt elicitácie sa hodnotil na základe obsahu derivátov kyseliny hydroxyškoricovej, vyjadrených ako kyseliny rozmarínová, v závislosti od koncentrácie použitého elicitora a dĺžky jeho pôsobenia. Spomedzi testovaných biotických elicitorov najúčinnejšie podporil produkčné vlastnosti suspenzných kultúr Melissa officinalis L. hydrolyzát z Botrytis cinerea (glukózový ekvivalent 24 mg.ml-1, 1 ml, doba pôsobenia 72 h). Obsah derivátov kyseliny hydroxyškoricovej sa po elicitácii zvýšil trojnásobne v porovnaní s neelicitovanými kultúrami. Produkčná schopnosť suspenzných kultúr elicitovaných metyljazmonátom sa mierne zvýšila len pri použití koncentrácie 10 μmol.l-1. Chlorid kobaltnatý a kademnatý efektívne stimulovali produkciu fenolových látok pri koncentrácii 100 μmol.l-1. Prítomnosť iónov kobaltu a kadmia v rastových médiách suspenzných kultúr viedla k dvoj-, resp. štvornásobnému zvýšeniu obsahu derivátov kyseliny hydroxyškoricovej.

Keywords: elicitation; Melissa officinalis L.; hydroxycinnamic acid derivatives; methyl jasmonate; Botrytis cinerea; metal ions

Kľúčové slová: elicitácia; Melissa officinalis L.; deriváty kyseliny hydroxyškoricovej; metyljazmonát; Botrytis cineream; ióny ťažkých kovov

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About the article

Received: 2014-11-30

Accepted: 2015-01-30

Published Online: 2015-06-29

Published in Print: 2015-06-01


Citation Information: Acta Facultatis Pharmaceuticae Universitatis Comenianae, ISSN (Online) 1338-6786, DOI: https://doi.org/10.1515/afpuc-2015-0012.

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© by A. Balažová. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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