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Agriculture (Pol'nohospodárstvo)

The Journal of National Agricultural and Food Centre

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Sodium Azide Induced Morphological and Molecular Changes in Persimmon (Diospyros Lotus L.)

Zuzana Kochanová
  • Slovak University of Agriculture, Nitra
  • Institute of Botany, Slovak Academy of Sciences, Bratislava
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Katarína Ražná / Elena Zuriaga / Maria Badenes / Ján Brindza
Published Online: 2012-07-16 | DOI: https://doi.org/10.2478/v10207-012-0007-6

Sodium Azide Induced Morphological and Molecular Changes in Persimmon (Diospyros Lotus L.)

Persimmon Diospyros lotus L. is an underutilized species, used as rootstock for Japanese persimmon (Diospyros kaki Thunb.) in many European countries. It has a potential for ornamental uses due to its diploid genome that suggests an easy mutation induction. This study was aimed at unravelling the effects of mutagenic sodium azide (in concentrations of 0.1% and 0.5%) on morphological characters (plant height, maximal leaf blade length, maximal leaf blade width and leaf petiole length) and molecular relations of treated accessions, based on AFLP (amplified fragment length polymorphism). In the M1 generation, the analysed characters of two-year old plants differed among the treatments. Sodium azide influenced persimmon stem and leaves in the opposite way, with increase up to 31.57% in plant height and decrease up to 30.33% in leaf petiole length. Changes in morphological characters were significant, that implies the importance of azide influence on the persimmon. Most genotypes treated with 0.1% azide were without significant differences among them and clustered together. The results suggest that the higher azide concentration of 0.5% is more suitable for persimmon seeds azide treatment to form genotypes, significantly different from the control, at the molecular level.

Keywords: AFLP; Diospyros lotus; mutagen; persimmon; sodium azide

  • AHLOOWALIA, B. S. - MALUSZYNSKI, M. 2001. Induced mutation. A new paradigm in plant breeding. In Euphytica, vol. 118, 2001, no. 2, pp. 167-173. DOI: 10.1023/A:1004162323428CrossrefGoogle Scholar

  • AL-QURAINY, F. 2009. Effects of sodium azide on growth and yield traits of Eruca sativa (L.). In World Applied Sciences Journal, vol. 7, 2009, no. 2, pp. 220-226.Google Scholar

  • AL-QURAINY, F. - KHAN, S. 2009. Mutagenic effects of sodium azide and its application in crop improvement. In World Applied Sciences Journal, vol. 6, 2009, no. 12, pp. 1589-1601.Google Scholar

  • AYAZ, A. - KADIOGLU, A. 1997. Changes in phenolic acid contents of Diospyros lotus L. during fruit development. In Journal of Agricultural and Food Chemistry, vol. 45, 1997, no. 7, pp. 2539-2541. DOI: 10.1021/jf960741cCrossrefGoogle Scholar

  • BADENES, M. - GARCES, A. - ROMERO, C. - ROMERO, M. - CLAVE, J. - ROVIRA, M. - LLACER, G. 2003. Genetic diversity of introduced and local Spanish persimmon cultivars revealed by RAPD markers. In Genetic Resources and Crop Evolution, vol. 50, 2003, no. 6, pp. 579-585. DOI: 10.1023/A:1024474719036CrossrefGoogle Scholar

  • CHEN, F. Y. - NI, H. Z. - HE, L. K. - WANG, Y. - LU, G. Y. - WANG, D. M. - REN, Z. C. 2011. Identification and genetic relationships analysis of some oriental persimmon (Diospyros kaki) and related species in Zhejiang province based on SSR analysis. In Journal of Fruit Science, vol. 22, 2011, no. 1, pp. 46-50.Google Scholar

  • CHO, D. H. - CHUN, I. J. - KWON, S. T. - SONG, Y. S. - CHOU, Y. D. 2007. Genetic relationships of Korean astringent persimmon varieties using AFLP analysis. In Korean Journal of Horticultural Science & Technology, vol. 25, 2007, no. 2, pp. 114-118.Google Scholar

  • DIVANLI-TURKAN, A. - KHAWARI, K. M. - CIFTCI, C. Y. - OZCAR, S. 2006. Effects of mutagenic sodium azide (NaN3) on in vitro development of four pea (Pisum sativum L.) cultivars. In International Journal of Agriculture & Biology, vol. 8, 2006, no. 3, pp. 349-353.Google Scholar

  • DU, X. Y. - ZHANG, Q. L. - LI, B. - LENG, P. - ZHANG, M. D. - HUANG, J. M. - LUO, Z. R. 2011. Mutations identification based on IRAP analysis in Diospyros kaki cv. Damopan. In Journal of Fruit Science, vol. 22, 2011, no. 2, pp. 257-262.Google Scholar

  • GRYGORIEVA, O. - KLYMENKO, S. - BRINDZA, J. - KOCHANOVA, Z. - TOTH, D. - DEREVJANKO, V. - GRA BOVECKA, O. 2009. Morphometrical analysis of Diospyros lotus population in the Mlyňany Arboretum, Slovakia. In Acta Horticulturae, 2009, no. 833, pp. 145-150.Google Scholar

  • GULLINO, P. - DEVECCHI, M. - LARCHER, F. 2009. The cultivation and ornamental uses of persimmon in Piedmontese gardens. In Acta Horticulturae, 2009, no. 833, pp. 83-88.Google Scholar

  • GUO, D. - ZHANG, H. - LUO, Z. 2006. Genetic relationships of Diospyros kaki Thunb. and related species revealed by IRAP and REMAP analysis. In Plant Science, vol. 170, 2006, no. 3, pp. 528-533. DOI: 10.1016/j.plantsci.2005.10.006CrossrefGoogle Scholar

  • GUO, D. L. - LUO, Z. R. 2006. Genetic relationships of some PCNA persimmons (Diospyros kaki Thunb.) from China and Japan revealed by SRAP analysis. In Genetic Resources and Crop Evolution, vol. 53, 2006, no. 53, pp. 1597-1603. DOI: 10.1007/s10722-005-8717-5CrossrefGoogle Scholar

  • ILBAS, A. I. - EROGLU, Y. - EROGLU, H. E. 2005. Effects of the application of different concentrations of NaN3 for different times on the morphological and cytogenetic characteristics of barley (Hordeum vulgare L.) seedlings. In Journal of Integrative Plant Biology, vol. 47, 2005, no. 9, pp. 1101-1106. DOI: 10.1111/j.1744-7909.2005.00137.xCrossrefGoogle Scholar

  • IRMAK, M. A. - YILMAZ, H. 2008. Determination of the usability of woody plant species in Tortum - Creek Watershed for functional and aesthetical uses in the respect of landscape architecture. In Biological Diversity and Conservation, vol. 1, 2008, no. 1, pp. 1-12.Google Scholar

  • JO, C. - SON, J. H. - SHIN, M. G. - BYUN, M. W. 2003. Irradiation effects on color and functional properties of persimmon (Diospyros kaki L. folium) leaf extract and licorice (Glycyrrhiza uralensis Fischer) root extract during storage. In Radiation Physics and Chemistry, vol. 67, 2003, no. 2, pp. 143-148. DOI: 10.1016/S0969-806X(02)00443-7CrossrefGoogle Scholar

  • KANZAKI, S. - YONEMORI, K. - SATO, A. - YAMADA, M. - SUGIURA, A. 2000. Analysis of the genetic relationships among pollination-constant and non-astringent (PCNA) cultivars of persimmon (Diospyros kaki Thunb.) from Japan and China using amplified fragment length polymorphism (AFLP). In Journal of the Japanese Society for Horticultural Science, vol. 69, 2000, no. 6, pp. 665-670. DOI: 10.2503/jjshs.69.665CrossrefGoogle Scholar

  • KHAN, S. - AL-QURAINY, F. 2009. Mutagenic effect of sodium azide on seed germination of Eruca sativa (L.). In Australian Journal of Basic and Applied Sciences, vol. 3, 2009, no. 4, pp. 3081-3087.Google Scholar

  • KHOSHBAKHT, K. - HAMMER, K. 2006. Savadkouh (Iran) an Evolutionary centre for fruit trees and shrubs. In Genetic Resources and Crop Evolution, vol. 53, 2006, no. 3, pp. 641-651. DOI: 10.1007/s10722-005-7467-8CrossrefGoogle Scholar

  • KIRUKI, S. - ONEK, L. A. - LIMO, M. 2006. Azide-based mutagenesis suppresses Striga hermonthica seed germination and parasitism on maize varieties. In African Journal of Biotechnology, vol. 5, 2006, no. 10, pp. 866-870. DOI: jb06156Google Scholar

  • LI, W. - OUYANG, Z. - MENG, X. - WANG, X. 2006. Plant species composition in relation to green cover configuration and function of urban parks in Beijing, China. In Ecological Research, vol. 21, 2006, no. 2, pp. 221-237. DOI: 10.1007/s11284-005-0110-5CrossrefGoogle Scholar

  • LOIZZO, M. R. - SAID, A. - TUNDIS, R. - HAWAS, U. W. - RASHED, K. - MENICHINI, F. - FREGA, N. G. - MENICHINI, F. 2009. Antioxidant and antiproliferative activity of Diospyros lotus L. extract and isolated compounds. In Plant Foods for Human Nutrition, vol. 64, 2009, no. 4, pp. 264-270. DOI: 10.1007/s11130-009-0133-0CrossrefGoogle Scholar

  • LUO, Z. R. - YONEMORI, K. - SUGIURA, A. 1995. Evaluation of RAPD analysis for cultivar identification of persimmons (Diospyros kaki). In Journal of the Japanese Society for Horticultural Science, vol. 64, 1995, no. 3, pp. 535-541.Google Scholar

  • MAKI, S. - OYAMA, K. - KURAHASHI, T. - NAKAHIRA, T. - KAWABATA, T. - YAMADA, T. 2001. RFLP analysis for cultivar identification of persimmon. In Scientia Horticulturae, vol. 91, 2001, no. 3-4, pp. 407-412. DOI: 10.1016/S0304-4238(01)00254-0CrossrefGoogle Scholar

  • MESSAOUDI, Z. - GMILI, R. E. - KHATIB, F. - HELMY, Y. 2009. Effect of pollination, fruit thinning and gibberellic acid application on ‘Fuyu’ kaki fruit development. In Acta Horticulturae, 2009, no. 833, pp. 233-237.Google Scholar

  • MITCHELL, G. E. - MCLAUCHLAN, R. L. - ISAACS, A. R. - WILLIAMS, D. J. - NOTTINGHAM, S. M. 1992. Effect of low dose irradiation on composition of tropical fruits and vegetables. In Journal of Food Composition and Analysis, vol. 5, 1992, no. 4, pp. 291-311. DOI: 10.1016/0889- 1575(92)90063-PCrossrefGoogle Scholar

  • MOLINA-CANO, J. L. - SIMIAND, J. P. - SOPENA, A. - PEREZ- VANDRELL, A. M. - DORSCH, S. - RUBIALES, D. - SWANSTON, J. S. - JAHOOR, A. 2003. Mildew-resistant mutants induced in North American two- and six-rowed malting barley cultivars. In Theoretical and Applied Genetics, vol. 107, 2003, no. 7, pp. 1278-1287. DOI: 10.1007/s00122-003-1362-5CrossrefGoogle Scholar

  • NABAVI, S. M. - EBRAHIMZADEH, M. A. - NABAVI, S. F. - FAZELIAN, M. - ESLAMI, B. 2009. In vitro antioxidant and free radical scavenging activity of Diospyros lotus and Pyrus boissieriana growing in Iran. In Pharmacognosy Magazine, vol. 5, 2009, no. 18, pp. 122-126.Google Scholar

  • NAVAL, M. M. - ZURIAGA, E. - PECCHIOLI, S. - LLÁCER, G. - GIORDANI, E. - BADENES, M. L. 2010. Analysis of genetic diversity among persimmon cultivars using microsatellite markers. In Tree Genetics & Genomes, vol. 6, 2012, no. 5, pp. 677-687. DOI: 10.1007/s11295-010-0283-0CrossrefWeb of ScienceGoogle Scholar

  • OZEN, A. - COLAK, A. - DINCER, B. - GUNER, S. 2004. A diphenolase from persimmon fruits (Diospyros kaki L., Ebenaceae). In Food Chemistry, vol. 85, 2004, no. 3, pp. 431-437. DOI: 10.1016/j.foodchem.2003.07.022CrossrefGoogle Scholar

  • PEARSON, O. W. - SANDER, C. - NILAN, R. A. 1975. The effect of sodium azide on cell processes in the embryonic barley shoot. In Radiation Botany, vol. 15, 1975, no. 4, pp. 315-322. DOI: 10.1016/0033-7560(75)90002-2CrossrefGoogle Scholar

  • PRADO, M. J. - GONZALEZ, M. V. - ROMO, S. - HERRERA, M. T. 2007. Adventitious plant regeneration on leaf explants from adult male kiwifruit and AFLP analysis of genetic variation. In Plant Cell, Tissue and Organ Culture, vol. 88, 2007, no. 1, pp. 1-10. DOI: 10.1007/s11240-006-9116-0Web of ScienceCrossrefGoogle Scholar

  • PRINA, A. R. - FAVRET, E. A. 1983. Parabolic effect in sodium azide mutagenesis in barley. In Hereditas, vol. 98, 1983, no. 1, pp. 89-94. DOI: 10.1111/j.1605223.1983.tb00583.xCrossrefGoogle Scholar

  • RINES, H. W. 1985. Sodium azide mutagenesis in diploid and hexaploid oats and comparison with ethyl methanesulfo nate treatments. In Environmental and Experimental Botany, vol. 25, 1985, no. 1, pp. 7-16. DOI: 10.1016/0098- 8472(85)90043-7CrossrefGoogle Scholar

  • SAID, A. - HAWAS, U. W. - NOFAL, S. M. - RASHED, K. - HUEFNER, A. 2009. Pharmaco-chemical studies on the aqueous methanolic extract of Diospyros lotus leaves. In Research Journal of Phytochemistry, vol. 3, 2009, no. 1, pp. 1-12. DOI: 10.3923/rjphyto.2009.1.12CrossrefGoogle Scholar

  • SAMADI, G. R. - MIAKHEL, I. M. - ABDIANI, S. A. 2009. Status of persimmon cultivation in Afghanistan. In Acta Horticulturae, 2009, no. 833, pp. 31-34.Google Scholar

  • SANDER, C. - MUEHLBAUER, F. J. 1977. Mutagenic effects of sodium azide and gamma irradiation in Pisum. In Environmental and Experimental Botany, vol. 17, 1977, no. 1, pp. 43-45. DOI: 10.1016/0098-8472(77)90019-3CrossrefGoogle Scholar

  • STENLID, G. 1949. Some notes on the effect of sodium azide, 2,4-dimtrophenol, and ortho-phenanthroline upon oxygen consumption in green leaves. In Physiologia Plantarum, vol. 2, 1949, no. 1, pp. 61-69. DOI: 10.1111/j.1399- 3054.1949.tb07649.xCrossrefGoogle Scholar

  • TAO, R. - GAO, M. - ESUMI, T. - KITAMURA, Y. - YAMADA, A. 2009. High frequency ploidy variation observed in seedlings of a hexaploid persimmon cultivar ‘Fujiwaragosho’. In Acta Horticulturae, 2009, no. 833, pp. 131-134.Google Scholar

  • VELEMINSKY, J. - ANGELIS, K. J. 1987. Effects of sodium azide on replicative and repair DNA synthesis in barley embryos. In Mutation Research, vol. 190, 1987, pp. 125-129. DOI: 10.1016/0165-7992(87)90043-1CrossrefGoogle Scholar

  • VOS, P. - HOGERS, R. - BLEEKER, M. - REIJANS, M. - LEE, T. - HORNES, M. - FRIJTERS, A. - POT, J. - PELEMAN, J. - KUIPER, M. - ZABEAU, M. 1995. AFLP: a new technique for DNA fingerprinting. In Nucleid Acids Research, vol. 23, 1995, no. 21, pp. 4407-4414.Google Scholar

  • YAMAGISHI, M. - MATSUMOTO, S. - NAKATSUKA, A. - ITAMURA, H. 2005. Identification of persimmon (Diospyros kaki) cultivars and phenetic relationships between Diospyros species by more effective RAPD analysis. In Scientia Horticulturae, vol. 105, 2005, no. 2, pp. 283-290. DOI: 10.1016/j.scienta.2005.01.020CrossrefGoogle Scholar

  • YAMANE, H. - ICHIKI, M. - TAO, R. - ESUMI, T. - YONEMORI, K. 2009. Fruit growth and development of small-fruit dwarf mutant in Japanese persimmon (Diospyros kaki Thunb.). In Acta Horticulturae, 2009, no. 833, pp. 125-130.Google Scholar

  • YAMASAKI, H. - SAKIHAMA, Y. 2000. Simultaneous production of nitric oxide and peroxynitrite by plant nitrate reductase: in vitro evidence for the NR-dependent formation of active nitrogen species. In FEBS Letters, vol. 468, 2000, no. 1, pp. 89-92.Google Scholar

  • YONEMORI, K. - HONSHO, C. - KITAJIMA, A. - AKIRA, K. - ARADHYA, M. - GIORDANI, E. - BELLINI, E. - PARFITT, D. E. 2008. Relationship of European persimmon (Diospyros kaki Thunb.) cultivars to Asian cultivars, characterized using AFLPs. In Genetic Resources and Crop Evolution, vol. 55, 2008, no. 1, pp. 81-89. DOI: 10.1007/s10722-007-9216-7CrossrefGoogle Scholar

About the article


Published Online: 2012-07-16

Published in Print: 2012-07-09


Citation Information: Agriculture, Volume 58, Issue 2, Pages 57–64, ISSN (Online) 1338-4376, ISSN (Print) 0551-3677, DOI: https://doi.org/10.2478/v10207-012-0007-6.

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