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Acta Horticulturae et Regiotecturae

The Scientific Journal for Horticulture, Landscape Engineering and Architecture

2 Issues per year

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Online
ISSN
1338-5259
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The Effect of Nitrogen and Sulphur Nutrition on the Yield and Content of Antioxidants in Broccoli / Vplyv Dusíkatej a Sírnatej Výživy na Úrodu a Obsah Antioxidantov v Brokolici

Miroslav Šlosár
  • Corresponding author
  • Slovak University of Agriculture in Nitra, Slovak Republic
  • Email:
/ Anton Uher
  • Slovak University of Agriculture in Nitra, Slovak Republic
/ Nina Čekey
  • Slovak University of Agriculture in Nitra, Slovak Republic
Published Online: 2013-09-06 | DOI: https://doi.org/10.2478/ahr-2013-0004

Abstract

The small field experiment with broccoli (variety ‚Tiburon F1‘) was established in The Botanical Garden of Slovak University of Agriculture in Nitra. In this experiment, we investigated the effect of four variants of nutrition on the broccoli yield, content of sulforaphane and vitamin C in broccoli florets. The first variant of nutrition was a control one - without application of fertilizers. The second one was fertilized at nitrogen level 200 kg.ha-1. The third one had level of nutriments N : S = 200 : 50 kg.ha-1 and fourth one N : S = 200 : 60 kg.ha-1. In each variant of nutrition, the yield of broccoli was significantly increased in comparison with the control variant. The highest yield of broccoli florets was reached in variant 4 (27.87 t.ha-1). The highest amount of sulforaphane was also determined in variant 4 - 50.93 mg.kg-1 of fresh matter. However, this value did not represent significant increase of sulforaphane content compared to the control variant. The highest content of vitamin C was determined in variant 2 (585.78 mg.kg-1 of fresh matter). In this variant, we achieved significant increase of vitamin C content in comparison with the control variant. Thus, by application of nitrogen and sulphur nutrition, we reached significant increase of broccoli yield compared to the control variant. At the same time, the applied nutrition assisted to enhanced accumulation of sulforaphane and vitamin C in broccoli florets

Maloparcelkový poľný pokus s brokolicou (odroda Tiburon F1) bol založený v roku 2008 v areáli Demonštračnej záhrady SPU v Nitre. V pokuse sme skúmali vplyv štyroch variantov výživy na úrodu, obsah sulforafanu a vitamínu C v ružiciach brokolice. Prvý variant (1) bol kontrolný - neboli na ňom aplikované priemyselné hnojivá. Variant 2 bol vyhnojený na úroveň N 200 kg.ha-1. Pri 3. a 4 variante boli živiny doplnené na úroveň N : S = 200 : 50 kg.ha-1, resp. 200 : 60 kg.ha-1. Na všetkých variantoch výživy sa úroda ružíc brokolice preukazne zvýšila v porovnaní s kontrolou. Najvyššia úroda ružíc brokolice bola dosiahnutá na variante 4 - 27,87 t.ha-1. Najväčšie množstvo sulforafanu bolo taktiež zistené vo variante 4 - 50,93 mg.kg-1 čerstvej hmoty. Táto hodnota však nepredstavovala preukazné zvýšenie obsahu sulforafanu oproti kontrolnému variantu. Najväčší obsah vitamínu C bol dosiahnutý na variante 2, pričom sme zaznamenali preukazné zvýšenie obsahu vitamínu v porovnaní s kontrolou. A teda, aplikáciou dusíkatej a sírnatej výživy sme dosiahli preukazné zvýšenie úrody oproti kontrolnému variantu, ale zároveň aplikovaná výživa prispela k zvýšenej kumulácii sulforafanu a vitamínu C v ružiciach brokolice

Keywords : broccoli; nutrition; nitrogen; sulforaphane; vitamin C

Kľúčové slová : brokolica; výživa; úroda; sulforafan; vitamín C; dusík; síra

  • BABIK, I. - ELKNER, K. 2002. The effect of nitrogen fertlization and irrigation on yield and quality of broccoli. In: Acta Horticulturae (ISHS), 2002, no. 571, p. 33-43. ISSN 0567-7572.Google Scholar

  • Byers , T. - Perry , G. 1992. Dietary carotenes, vitamin C and vitamin E as protective antioxidants in human cancers. In: Annual review of Nutrition, vol. 12, 1992, p. 139-159. ISSN 0199-9885.Google Scholar

  • DUCSAY, L. - VARGA, L. 2001. Vplyv dusíkatého hnojenia na tvorbu úrody, vitamínu C a akumuláciu dusičnanov pri pekinskej kapuste. In: Acta horticulturae et regiotecturae, roč. 7, 2001, č. 1, s. 20-23. ISSN 1335-2563.Google Scholar

  • FAHEY, J. W. -HARISTOY, X. - DOLAN, P. M. - KENSLER, T. W. - SCHOLTUS, I. - STEPHENSON, K. K. - TALALAY, P. - LOZNIEWSKI, A. 2002. Sulforaphane inhibits extracellular, intracellular, and antibiotic-resistant strains of Helicobacter pylori and prevents benzo[a]pyrene- -induced stomach tumors. In: Proceedings of the National Academy of Sciences of The United States of America, vol. 99, 2002, no. 11, p. 7610-7615. ISSN 0027-8424.Google Scholar

  • FECENKO, J. - LOŽEK, O. 2000. Výživa a hnojenie poľných plodín. Nitra : SPU, 2000. 452 s. ISBN 80-7137-777-5.Google Scholar

  • HLUŠEK, J. - RICHTER, R. - RYANT, P. - KOLÁŘ, L. Výživa a hnojení zahradních plodin. Praha : Zemědělec, 2002, 94 s. ISBN 80-902413-5-2.Google Scholar

  • HOLLÓSY, M. 2004. Dajme zelenú brokolici! In: APOTHÉKA - odborno- informačný štvrťročník pre zákazníkov lekárne, roč. 4, 2004, č. 2, s. 48-49. ISSN 1336-0930.Google Scholar

  • JOSEPH, M. A. - MOYSICH, K. B. - FREUDENHEIM, J. L. - SHIELDS, P. G. - BOWMAN, E. D. -ZHANG, Y. - MARSHALL, J. R. - AMBROSONE, C. B. 2004. Cruciferous vegetables, genetic polymorphisms in glutathione S-transferases M1 and T1, and prostate cancer risk. In: Nutrition and cancer, vol. 50, 2004, no. 2, p. 206-213. ISSN 0163-5581.Google Scholar

  • KARITONAS, R. 2002 Effect of nitrogen supply on yield and quality of broccoli. In: Plant nutrition, vol. 92, 2002, p. 298-299. ISSN 978-0-7923-7105-2.Google Scholar

  • KUSHAD, M. M. - BROWN, A. F. - KURILICH, A. C. - JUVIK, J. A. - KLEIN, B. P. - WALLIG, M. A. - JEFFERY, E. H. 1999. Variation of glucosinolates in vegetable crops of Brassica oleracea. In: Journal of agricultural and food chemistry, vol. 47, 1999, no. 4, p. 1541-1548. ISSN 0021-8561.Google Scholar

  • LISIEWSKA, Z. - KMIECIK, W. 1996. Effects of level of nitrogen fertilizer, processing conditions and period of storage of frozen broccoli and cauliflower on vitamin C retention. In: Food Chemistry, vol. 57, 1996, no. 2, p. 267-270. ISSN 0308-8146.CrossrefGoogle Scholar

  • POKLUDA, R. 2006. Význam a nutriční hodnota zeleniny. In: Zahradnictví, roč. 10, 2006, č. 6, s. 18-19. ISSN 1213-7596Google Scholar

  • Sivakumar , G. - Aliboni, A. - Bacchetta , L. 2007. HPLC screening of anti-cancer sulforaphane from important European Brassica species. In: Food Chemistry, vol. 104, 2007, no. 4, p. 1761-1764. ISSN 0308-8146.CrossrefGoogle Scholar

  • Spitz, M. R. - Duphorne, c. m. - Detry , M a. - Pillow , P. c. - Amos, c. i. - Lei Lei - de Andrade , m. - Xiangjun Gu - Hong, W. k - Xifeng Wu. 2000. Dietary intake of isothiocyanates: evidence of a joint effect with glutathione S-transferase polymorphisms in lung cancer risk. In: Cancer Epidemiology Biomarkers and Prevention, vol. 9, 2000, no. 10, p. 1017-1020. ISSN 1055-9965.Google Scholar

  • ŠLOSÁR, M. - ČEKEY, N. 2008. Hlúbová zelenina - významná súčasť výživy človeka. In: Zahradnictví, roč. 12, 2008, č. 8, s. 16-17. ISSN 1213-7596Google Scholar

  • VARGA, L. - LOŽEK, O. - DUCSAY, L. 2004. Vplyv diferencovanej výživy na úrodu brokolice. In: 2. medzinárodná záhradnícka vedecká konferencia, 16.-18. september 2004, Nitra : SPU, 2004, s. 96-98. ISSN 1335-2563Google Scholar

About the article

Published Online: 2013-09-06

Published in Print: 2013-09-01


Citation Information: Acta Horticulturae et Regiotectuare, ISSN (Online) 1338-5259, DOI: https://doi.org/10.2478/ahr-2013-0004.

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