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Acta Horticulturae et Regiotecturae

The Scientific Journal for Horticulture, Landscape Engineering and Architecture

2 Issues per year

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Online
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1338-5259
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OPTIMISATION OF GROWING CONDITIONS OF EUROPEAN MISTLETOE HYPERPARASITIC FUNGUS (PHAEOBOTRYOSPHAERIA VISCI): EFFECT OF DIFFERENT MEDIA AND ANTIBIOTICS / OPTIMALIZÁCIA RASTOVÝCH PODMIENOK PHAEOBOTRYOSPHAERIA VISCI, HYPERPARAZITICKEJ HUBY IMELA BIELEHO: VPLYV RÔZNYCH ŽIVNÝCH MÉDIÍ A ANTIBIOTÍK

Ildikó Varga
  • Corresponding author
  • Institute of Plant Protection, Georgikon Faculty, University of Pannonia, Hungary
  • University of Helsinki, Helsinki, Finland2
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/ Tivadar Baltazár / Miloš Pejchal
Published Online: 2013-12-13 | DOI: https://doi.org/10.2478/ahr-2013-0011

Abstract

European mistletoe (Viscum album) is an evergreen, perennial, hemiparasitic shrub, which is able to infect more than 450 woody species. Besides mechanical pruning, a hyperparasitic fungus (Phaeobotryosphaeria visci) could be a successful candidate to develop an effective biocontrol agent against mistletoe. It was necessary to find antibiotics which make the isolation easier and do not effect mycelia growth negatively under strain. Furthermore, we wanted to define a medium for optimal growth under in vitro laboratory conditions. Three different media (potato-dextrose agar, modified oatmeal agar, ¼ PDA + V8 agar) and four antibiotics (kanamycin, ampicillin, rifampicin, nystatin) were tested. Oatmeal agar and potato-dextrose agar are optimal to maintain the fungal strains, the colony diameter was 9 cm on the 12th day. All tested antibiotics are useful, except nystatin.

Súhrn

Imelo biele (Viscum album) je vždyzelený viacročný poloparazitický ker schopný napadnúť vyše 450 druhov drevín. Okrem mechanickej redukcie imela existuje možnosť vyvinúť proti nemu účinný biologický kontrolný prostriedok použitím hyperparazitickej huby (Phaeobotryosphaeria visci). Na zavedenie tejto metódy bolo nevyh- nutné identifikovať antibiotiká, ktoré umožnia jednoduchú izoláciu huby a nebudú mať negatívny vplyv na rast mycélia. Chceli sme tiež nájsť živné médium, ktoré by umožňovalo optimálny rast v laboratórnych podmienkach in vitro. Testovali sme tri druhy živných médií (zemiakovo-dextrózový agar, modifikovaný ovsený agar a ¼ PDA+V8 agar) a štyri druhy antibiotík (kanamycín, ampicilín, rifampicín a nystatín). Modifikovaný ovsený agar a zemiakovo-dextrózový agar zabezpečili optimálny rast, stredná hodnota priemeru kolónií na dvanásty deň dosiahla 9 cm. Všetky testované antibiotiká s výnimkou nystatínu boli na zvolený účel vhodné.

Keywords : Botryosphaeriaceae; oatmeal agar; antibiotic sensitivity; biological control

Kľúčové slová : Botryosphaeriaceae; ovsený agar; citlivosť na antibiotiká; biologická kontrola

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About the article

Published Online: 2013-12-13

Published in Print: 2013-12-01


Citation Information: Acta Horticulturae et Regiotectuare, ISSN (Online) 1338-5259, DOI: https://doi.org/10.2478/ahr-2013-0011.

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