The grapevine moth (Lobesia botrana) and the grape berry moth (Eupoecilia ambiguella) are the main pests in southern Slovakia vineyards. While L. botrana represents the most common species in the vineyards, E. ambiguella exists in some sites only. In this study we monitored the occurrence and dynamics of the flights of the grapevine moth and the grape berry moth in the vineyard area Dvory nad Žitavou. The size of the examined area was 60 ha in 2008 and 90 ha in 2009. On these areas, we used pheromone traps type Deltastop LB for grapevine moth and Deltastop EA for grape berry moth. To control the movement of the pests, a set of three pheromone traps for each type was used in observation points which were placed at least 50 m from each other. Monitoring was carried out regularly every second to third day. The control area was located in immediate vicinity of the area. As a threshold, we consider 20 moths for 2 days at an average of three pheromone traps. In 2008, we recorded the first, second and third generation of Lobesia botrana and only the second generation of Eupoecilia ambiguella. In 2009, we saw all three generations for both types of moths. We also examined effectiveness of the protection method of grapevine based on the mating disruption using pheromone dispensers type ISONET L plus. Just before the anticipated start of swarming we distributed 420 dispensers/ha evenly over the entire vineyard; on the edges of the treated area, the basic dose of dispensers i.e. 500 pieces/ha was left. To assess the effectiveness of the product ISONET L plus in 2008 we reached more than 70% efficiency in the border plots, inside 96%. We achieved 69% efficiency in 2009. Based on the results it is be clear that the long-term use of this method of protection has a positive impact in reducing the population of moth. It is suitable for use in an integrated and biological protective system against Lobesia botrana and Eupoecilia ambiguella.