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Archives of Industrial Hygiene and Toxicology

The Journal of Institute for Medical Research and Occupational Health

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0004-1254
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Volume 62, Issue 2 (Jun 2011)

Issues

The Effects of Hyaluronic Acid, Calcium Hydroxide, and Dentin Adhesive on Rat Odontoblasts and Fibroblasts

Ana Bogović / Jana Nižetić / Nada Galić / Davor Želježić / Vedran Micek / Marin Mladinić
Published Online: 2011-06-24 | DOI: https://doi.org/10.2478/10004-1254-62-2011-2076

The Effects of Hyaluronic Acid, Calcium Hydroxide, and Dentin Adhesive on Rat Odontoblasts and Fibroblasts

The aim of this study was to investigate the effects and efficiency of pulp capping preparations based on hyaluronic acid, calcium hydroxide, and dentin adhesive on the pulp tissue of Sprague-Dawley rats. The rats were killed and extracted teeth sectioned transversely through the pulp. The slices were placed in a RPMI 1640 cell culture medium supplemented with 10 % foetal calf serum. During 14 days of cultivation cultures were treated with preparations that contained hyaluronic acid (Gengigel Prof®), and calcium hydroxide (ApexCal®), or with dentin adhesive (Excite®). Cellularity and viability of fibroblasts and odontoblasts was analysed using a haemocytometer. Hyaluronic acid proved most efficient and the least toxic for direct pulp capping. Even though calcium hydroxide and dentin adhesive demonstrated a higher degree of cytotoxicity, their effects were still acceptable in terms of biocompatibility.

Utjecaj hijaluronske kiseline, kalcijeva hidroksida i dentinskih adheziva na odontoblaste i fibroblaste štakora

Cilj ovog rada bio je istražiti djelovanje preparata na bazi hijaluronske kiseline i kalcijeva hidroksida te dentinskog adheziva na pulpno tkivo Sprague-Dawley štakora u svrhu procjene učinkovitosti navedenih materijala kod direktnog prekrivanja pulpe. Izvađeni zubi transverzalno su podijeljeni kroz pulpu. Naresci su uzgajani u RPMI 1640 staničnom mediju obogaćenom s 10 % fetalnoga telećeg seruma u plastičnim bočicama za staničnu kulturu. Kulture su tijekom 14 dana tretirane preparatima s hijaluronskom kiselinom (Gengigel Prof®), kalcijevim hidroksidom (ApexCal®) i dentinskim adhezivom (Excite®). Nakon 14 dana pristupilo se analizi staničnosti i vijabilnosti s pomoću hemocitometra. Iako su preparati na bazi kalcijeva hidroksida i dentinski adheziv pokazali nešto viši stupanj citotoksičnosti, dobiveni su rezultati u granicama biokompatibilnosti. Primjena preparata na bazi hijaluronske kiseline postigla je najbolje rezultate te se ovaj materijal pokazao najboljim za direktno prekrivanje pulpe između tri ispitivana preparata.

Keywords: direct pulp capping; fibroblast proliferation; odontoblastic proliferation

Keywords: direktno prekrivanje pulpe; proliferacija fibroblasta; proliferacija odontoblasta

  • Walton RE, Torabinejad M. Principles and Practice of Endodontics. Philadelphia: W.B. Saunders Co.; 2002.Google Scholar

  • Schröder U. Effect of calcium hydroxide-containing pulp-capping agents on pulp cell migration, proliferation, and differentiation. J Dent Res 1985;64(Special No):541-8.Google Scholar

  • Tziafas D. Mechanisms controlling secondary initiation of dentinogenesis: a review. Int Endod J 1994;27:61-74.CrossrefPubMedGoogle Scholar

  • Gala-Garcia A, Teixeira KI, Wykrota FH, Sinisterra RD, Cortés ME. Bioceramic/poly (glycolic)-poly (lactic acid) composite induces mineralized barrier after direct capping of rat tooth pulp tissue. Braz Oral Res 2010;24:8-14.CrossrefGoogle Scholar

  • Chan C, Lan W, Chang M, Chen Y, Lan W, Chang H, Jeng JH. Effects of TGF-ßs on the growth, collagen synthesis and collagen lattice contraction of human dental pulp fibroblasts in vitro. Arch Oral Biol 2005;50:469-79.CrossrefGoogle Scholar

  • Kim JK, Shukla R, Casagrande L, Sedgley C, Nör JE, Baker JR Jr, Hill EE. Differentiating dental pulp cells via RGD-dendrimer conjugates. J Dent Res 2010;89:1433-8.Web of ScienceCrossrefPubMedGoogle Scholar

  • Bränström M, Garberoglio R. The dentinal tubules and the odontoblast processes. A scanning electron microscopic study. Acta Odontol Scand 1972;30:291-311.CrossrefGoogle Scholar

  • Modena KC, Casas-Apayco LC, Atta MT, Costa CA, Hebling J, Sipert CR, Navarro MF, Santos CF. Cytotoxicity and biocompatibility of direct and indirect pulp capping materials. J Appl Oral Sci 2009;17:544-54.PubMedCrossrefGoogle Scholar

  • Parolia A, Kundabala M, Rao NN, Acharya SR, Agrawal P, Mohan M, Thomas M. A comparative histological analysis of human pulp following direct pulp capping with Propolis, mineral trioxide aggregate and Dycal. Aust Dent J 2010;55:59-64.PubMedWeb of ScienceCrossrefGoogle Scholar

  • da Silva LA, de Freitas AC, de Carvalho FK, de Querioz AM, Nelson-Filho P, Porto-Neto ST. Direct pulp capping with a self-etching adhesive system: histopathologic evaluation in dogs' teeth. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2009;108:e34-e40.Web of ScienceGoogle Scholar

  • Shayegan A, Petein M, Vanden Abbeele A. The use of beta-tricalcium phosphate, white MTA, white Portland cement and calcium hydroxide for direct pulp capping of primary pig teeth. Dent Traumatol 2009;25:413-9.CrossrefPubMedWeb of ScienceGoogle Scholar

  • Pitt Ford TR, Roberts GJ. Immediate and delayed direct pulp capping with the use of a new visible light-cured calcium hydroxide preparation. Oral Surg Oral Med Oral Pathol 1991;71:338-42.Google Scholar

  • Zanchi CH, Lund RG, Perrone LR, Ribeiro GA, del Pino FA, Pinto MB, Demarco FF. Microtensile bond strength of two-step etch-and-rinse adhesive systems on sound and artificial caries-affected dentin. Am J Dent 2010;23:152-6.Google Scholar

  • Rodrigues Accorinte M de L, Loguercio AD, Reis A, Muench A, Araújo VC. Adverse effects of human pulps after direct pulp capping with the different components from a total-etch three-step adhesive system. Dent Mater 2005;21:599-607.CrossrefGoogle Scholar

  • Moraes Porto ICC, Andrade AK, Guēnes GM, Ribeiro AI, Braz R, Castro CM. In vitro potential cytotoxicity of an adhesive system to alveolar macrophages. Braz Dent J 2009;20:195-200.Google Scholar

  • Franz A, König F, Lucas T, Watts DC, Schedle A. Cytotoxicity effects of dental bonding substances as a function of degree of conversion. Dent Mater 2009;25:232-9.CrossrefPubMedWeb of ScienceGoogle Scholar

  • Çetingüç A, Ölmez S, Vural N. HEMA diffusion from dentin bonding agents in young and old primary molars in vitro. Dent Mater 2007;23:302-7.Web of ScienceCrossrefGoogle Scholar

  • Huang FM, Chang YC. Cytotoxicity of dentine-bonding agents on human pulp cells in vitro. Int Endod J 2002;35:905-9.CrossrefWeb of ScienceGoogle Scholar

  • Mantellini MG, Botero TM, Yaman P, Dennison JB, Hanks CT, Nor JE. Adhesive resin induces apoptosis and cell-cycle arrest of pulp cells. J Dent Res 2003;82:592-6.CrossrefPubMedGoogle Scholar

  • Becher R, Kopperud HM, Al RH, Samuelsen JT, Morisbak E, Dahlman HJ, Lilleaas EM, Dahl JE. Pattern of cell death after in vitro exposure to GDMA, TEGDMA, HEMA and two compomer extracts. Dent Mater 2006;22:630-40.PubMedCrossrefGoogle Scholar

  • Paranjpe A, Zhang H, Johnson JD. Effects of mineral trioxide aggregate on human dental pulp cells after pulp-capping procedures. J Endod 2010;36:1042-7.CrossrefGoogle Scholar

  • Hanks CT, Strawn SE, Wataha JC, Craig RG. Cytotoxic effects of resin components on cultured mammalian fibroblasts. J Dent Res 1991;70:1450-5.PubMedCrossrefGoogle Scholar

  • Kiba W, Imazato S, Takahashi Y, Yoshioka S, Ebisu S, Nakano T. Efficacy of polyphasic calcium phosphates as a direct pulp capping material. J Dent 2010;38:828-37.PubMedCrossrefWeb of ScienceGoogle Scholar

  • Huang GT-J, Takayoshi Y, Shea LD, Djouad F, Nastaran ZK, Tuan RS, Shi S. Stem/Progenitor cell-mediated de novo regeneration of dental pulp with newly deposited continuous layer of dentin in an in vivo model. Tissue Engin A 2010;16:605-15.Google Scholar

  • Weigel PH, Hascall VC, Tammi M. Hyaluronan synthases. J Biol Chem 1997;272:13997-14000.CrossrefPubMedGoogle Scholar

  • Toole BP, Hascall VC. Hyaluronan and tumor growth. Am J Pathol 2002;161:745-7.PubMedCrossrefGoogle Scholar

  • Kennedy JF, Phillips GO, Williams PA, Hascall VC, editors. Hyaluronan. 1st ed. Cambridge: Woodhead Publishing Ltd; 2002.Google Scholar

  • de la Motte C, Nigro J, Vasanji A, Rho H, Kessler S, Bandyopadhyay S, Danese S, Fiocchi C, Stern R. Platelet-derived hyaluronidase 2 cleaves hyaluronan into fragments that trigger monocyte-mediated production of proinflammatory cytokines. Am J Pathol 2009;174:2254-64.Web of SciencePubMedGoogle Scholar

  • Volpi N, Schiller J, Stern R, Soltés L. Role, metabolism, chemical modifications and applications of hyaluronan. Curr Med Chem 2009;16:1718-45.CrossrefPubMedGoogle Scholar

  • Duke RC, Cohen JJ. Morphological and biochemical assays of apoptosis. In: Coligan JE, Kruisbeak AM, editors. Current protocols in immunology. New York (NY): John Wiley & Sonds; 1992. p. 3.17.1-16.Google Scholar

  • Kostoryz EL, Eick JD, Chappelow CC, Glaros AG, Wetmore L, Yourtee DM. In vitro effect of light-cure dental adhesive on IL-6 release from LPS-stimulated and unstimulated macrophages. J Biomed Mater Res 2003;65:89-94.Google Scholar

  • Tronstad L, Andreasen JO, Hasselgren G, Kristerson L, Riis I. pH changes in dental tissues after root canal filling with calcium hydroxide. J Endod 1981;7:17-21.CrossrefGoogle Scholar

  • Heithersay GS. Calcium hydroxide in the treatment of pulpless teeth with associated pathology. J Brit Endod Soc 1975;8:74-93.CrossrefGoogle Scholar

  • Schröder U, Granath LE. Early reaction of intact human teeth to calcium hydroxide following experimental pulpotomy and its significance to the development of hard tissue barrier. Odont Revy 1971;22:379-95.Google Scholar

  • Uitto VJ, Antila R, Ranta R. Effects of topical glucocorticoid medication on collagen biosynthesis in the dental pulp. Acta Odontol Scand 1975;33:287-98.CrossrefPubMedGoogle Scholar

  • Fitzgerald M. Cellular mechanisms of dentinal bridge repair using 3H-thimidine. J Dent Res 1979;58:2198-206.Google Scholar

  • Torneck CD, Moe H, Howley TP. The effect of calcium hydroxide solution on porcine pulp fibroblasts in vitro. J Endod 1983;9:131-6.CrossrefGoogle Scholar

  • Tziafas D, Veis A, Alvanou A. Inability of calcium hydroxide to induce reparative dentinogenesis at non-peripheral sites of dog dental pulp. Eur J Oral Sci 1996;104:623-6.PubMedCrossrefGoogle Scholar

  • Pereira JC, Segala AD, Costa CA. Human pulpal response to direct pulp capping with an adhesive system. Am J Dent 2000;13:139-47.PubMedGoogle Scholar

  • Wiegand A, Buchholz K, Werner C, Attin T. In vitro cytotoxicity of different desensitizers under simulated pulpal flow conditions. J Adhes Dent 2008;10:227-32.Google Scholar

  • Demirci M, Hiller KA, Bosi C, Galler K, Schmalz G, Schweikl H. The induction of oxidative stress, cytotoxicity, and genotoxicity by dental adhesives. Dent Mater 2008;24:362-71.CrossrefWeb of SciencePubMedGoogle Scholar

  • Spagnuolo G, Galler K, Schmalz G, Cosentino C, Rengo S, Schweikl H. Inhibition of phosphatdylinositol 3-kinase amplifies TEGDMA-induced apoptosis in primary human pulp cells. J Dent Res 2004;83:703-7.Google Scholar

  • Spagnuolo G, Mauro C, Leonardi A, Santilo M, Paterno R, Schweikl H, Avvedimento EV, Rengo S. NF-kappaB protetction against apoptosis induced by HEMA. J Dent Res 2004;83:837-42.PubMedCrossrefGoogle Scholar

  • West DC, Kumar S. Hyaluronan and angiogenesis. In: Evered D, Whelan J, editors. Ciba Foundation Symposium 143 - The Biology of Hyaluronan. New York (NY): John Wiley & Sons Ltd.; 1989. p. 187-201.Google Scholar

  • Stern R, Asari AA, Sugahara KN. Size-specific fragments of hyaluronan: an information-rich system. Eur J Cell Biol 2006;85:699-715.CrossrefGoogle Scholar

  • Sasaki T, Kawamata-Kido H. Providing an environment for reparative dentine induction in amputated rat molar pulp by high molecular-weight hyaluronic acid. Arch Oral Biol 1995;40:209-19.PubMedCrossrefGoogle Scholar

About the article


Published Online: 2011-06-24

Published in Print: 2011-06-01


Citation Information: Archives of Industrial Hygiene and Toxicology, ISSN (Print) 0004-1254, DOI: https://doi.org/10.2478/10004-1254-62-2011-2076.

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