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Archives of Industrial Hygiene and Toxicology

The Journal of Institute for Medical Research and Occupational Health

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Volume 62, Issue 2 (Jun 2011)

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Determination of Artificial Sweeteners in Beverages and Special Nutritional Products Using High Performance Liquid Chromatography

Maja Serdar
  • Environmental Health Service, Croatian National Institute of Public Health
/ Zorka Knežević
  • Croatian Veterinary Institute, Zagreb, Croatia
Published Online: 2011-06-24 | DOI: https://doi.org/10.2478/10004-1254-62-2011-2084

Determination of Artificial Sweeteners in Beverages and Special Nutritional Products Using High Performance Liquid Chromatography

This paper presents two high performance liquid chromatographic (HPLC) methods used for the separation and determination of artificial sweeteners aspartame, acesulphame K, sodium saccharin, and sodium cyclamate in beverages and special nutritional products (special food intended for specific population groups). All four compounds are soluble in aqueous solutions and can easily be separated and determined by HPLC with a diode array detector (DAD). The first method involved separation of aspartame, acesulphame K, and sodium saccharin on a C18 column with an isocratic elution of phosphate buffer and acetonitrile as mobile phase. The second method was used to separate sodium cyclamate on a C18 column with methanol and water as mobile phase. Under optimum conditions, both methods showed good analytical performance, such as linearity, precision, and recovery. The methods were successfully applied for the analysis of real samples of soft drinks and special nutritional products.

Određivanje umjetnih sladila u osvježavajućim bezalkoholnim pićima i posebnim prehrambenim proizvodima metodom tekućinske kromatografije visoke djelotvornosti

Ovaj rad predstavlja dvije metode za separaciju i određivanje umjetnih sladila aspartama, acesulfama K, natrijeva saharina i natrijeva ciklamata u osvježavajućim bezalkoholnim pićima i posebnim prehrambenim proizvodima tehnikom tekućinske kromatografije visoke djelotvornosti (HPLC). Sva četiri analita topljiva su u vodenim otopinama i lako se mogu razdvojiti i odrediti tekućinskom kromatografijom uz primjenu detektora s nizom dioda (DAD). Prva je metoda kromatografsko razdvajanje aspartama, acesulfama K i natrij saharina na C18 koloni izokratnim ispiranjem fosfatnog pufera i acetonitrila kao mobilne faze. Druga metoda omogućava određivanje natrijeva ciklamata na C18 koloni sa metanolom i vodom kao mobilnom fazom. Pod najpovoljnijim eksperimentalnim uvjetima obadvije metode pokazale su dobre analitičke karakteristike, kao što su linearnost, preciznost i analitički povrat. Navedene su metode primijenjene za analize realnih uzoraka osvježavajućih bezalkoholnih pića i hrane za posebne prehrambene potrebe.

Keywords: acesulphame K; aspartame; sodium cyclamate; sodium saccharin; validation

Keywords: acesulfam K; aspartam; natrijev ciklamat; natrijev saharin; validacija

  • Zygler A, Wasik A, Namiesnik J. Analytical methodologies for determination of artificial sweeteners in foodstuffs. TrAC 2009;28:1082-1102.

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  • Zhu Y, Guo Y, Ye M. James F.S. Separation and simultaneous determination of four artificial sweeteners in food and beverages by ion chromatography. J Chromatogr A 2005;1085:143-146.

  • Pesek J.J, Matyska M.T. Determination of aspartame by high-performance capillary electrophoresis. J Chromatogr A 1997;781:423-428.

  • Cantarelli M.A, Pellerano R.G, Marchevsky E.J, Camina J.M. Simultaneous determination of aspartame and acesulfame-K by molecular absorption spectrophotometry using multivariate calibration and validation by high performance liquid chromatography. Food Chem 2009;115:1128-1132.

  • Horie M, Ishikawa F, Oishi M, Shindo T, Yasui A, Ito K. Rapid detrmination of cyclamate in foods by solid-phase exstraction and capillary electrophoresis. J Chromatogr A 2007;1154:423-428.

  • Huang Z, Ma J, Chen B, Zhang Y, Yao S. Determination of cyclamate in foods by high performance liquid chromatography-electrospray ionization mass spectrometry. Anal Chim Acta 2006;555:233-237.

  • Directive 94/35/EC of European Parliament and of the Council of 30 June 1994 on sweeteners for use in foodstuffs. OJEC 1994, L237/13.

  • Directive 96/83/EC of European Parliament and of the Council of 9 December 1996 amending Directive 94/35/EC on sweeteners for use in foodstuffs. OJEU 1997, L048, 16.

  • Directive 2003/115/EC of European Parliament and of the Council of 22 December 2003 amending Directive 94/35/EC on sweeteners for use in foodstuffs. OJEU 2004, L024, 65.

  • Directive 2006/52/EC of European Parliament and of the Council of 5 July 2006 amending Directive 95/2/EC on food additives other than colours and sweeteners and Directive94/35/EC on sweeteners for use in foodstuffs. OJEU 2004, L204, 10.

  • Pravilnik o prehrambenim aditivima [Food additives regulations in Croatian] Narodne novine 81/2008.

  • EN 12856:1999 - Determination of acesulfame-K, aspartame and saccharin by HPLC method.

  • EN 12857:1999 - Determination of cyclamate by HPLC method.

About the article


Published Online: 2011-06-24

Published in Print: 2011-06-01



Citation Information: Archives of Industrial Hygiene and Toxicology, ISSN (Print) 0004-1254, DOI: https://doi.org/10.2478/10004-1254-62-2011-2084. Export Citation

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