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Archives of Industrial Hygiene and Toxicology

The Journal of Institute for Medical Research and Occupational Health

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Volume 63, Issue 4 (Dec 2012)


Control of T-2 Toxin in Fusarium Langsethiae and Geotrichum Candidum Co-Culture / Kontrola Toksina T-2 U Kokulturi Fusarium Langsethiae I Geotrichum Candidum

Elida Gastélum-Martínez PhD
  • Corresponding author
  • Université de Toulouse, INPT-ENSAT, Laboratoire de Génie Chimique Castanet-Tolosan, CNRS, Laboratoire de Génie Chimique1,2, Toulouse, France
  • Centro de Investigacion y Asistencia en Tecnologia y Diseno del Estado de Jalisco, A. C. Unidad Sureste Calle 30 No. 151 (7 y 7A) Col. Garcia Gineres C. P. 97070, Merida, Yucatan, Mexico
  • Email:
/ Stephane Compant
  • Université de Toulouse, INPT-ENSAT, Laboratoire de Génie Chimique Castanet-Tolosan, CNRS, Laboratoire de Génie Chimique1,2, Toulouse, France
/ Patricia Taillandier
  • Université de Toulouse, INP-ENSIACET, Laboratoire de Génie Chimique, CNRS, Laboratoire de Génie Chimique1,2, Toulouse, France
/ Florence Mathieu
  • Université de Toulouse, INPT-ENSAT, Laboratoire de Génie Chimique Castanet-Tolosan, CNRS, Laboratoire de Génie Chimique1,2, Toulouse, France
Published Online: 2013-01-16 | DOI: https://doi.org/10.2478/10004-1254-63-2012-2206


Due to contamination of barley grains by Fusarium langsethiae, T-2 toxin can be present in the brewing process. It has been observed that the presence of the yeast Geotrichum candidum during malting can reduce the final concentration of this mycotoxin in beer. In this work, a co-culture method was carried out for both microorganisms in order to evaluate the effect on T-2 mycotoxin concentration in comparison with the pure culture of F. langsethiae in the same conditions. The microbial growth of both microorganisms was assessed using three different methods: dry weight, DOPE-FISH, and DNA quantification. In coculture, both microorganisms globally developed less than in pure cultures but G. candidum showed a better growth than F. langsethiae. The concentration of T-2 was reduced by 93 % compared to the pure culture. Hence, the interaction between G. candidum and F. langsethiae led to a drastic mycotoxin reduction despite the only partial inhibition of fungal growth

Zbog kontaminacije zrna ječma kulturom Fusarium langsethiae u postupku proizvodnje piva može doći do pojave T-2-toksina. Uočeno je da prisutnost plijesni Geotrichum candidum tijekom faze pretvaranja ječma u slad može sniziti krajnju koncentraciju ovog mikotoksina u pivu. U ovom je radu izvršena kokultura obaju mikroorganizama kako bi se procijenio učinak na koncentraciju T-2-mikotoksina u odnosu na čistu kulturu F. langsethiae u istim uvjetima. Mikrobni rast obaju mikroorganizama procijenjen je s pomoću triju metoda: mjerenjem suhe mase, fluorescencijskom in situ hibridizacijom s dvostruko obilježenim oligonukleotidnim sondama (Double-labeling of Oligonucleotide Probes for Fluorescent in situ Hybridisation - DOPE-FISH) i kvantifikacijom DNA. Ukupan razvoj obaju mikroorganizama u kokulturi bio je manji nego u pojedinačnim kulturama, ali je G. candidum pokazao nešto veći rast od F. langsethiae. Koncentracija T-2 snizila se za 93 % u usporedbi s čistom kulturom. Dakle, interakcija između G. candidum i F. langsethiae dovela je do drastičnog smanjenja koncentracije mikotoksina unatoč samo djelomičnoj inhibiciji fungalnog rasta.

KEYWORDS : brewing; contamination; growth of microorganisms; mycotoxins

KEYWORDS : kontaminacija; mikotoksini; proizvodnja piva; rast mikroorganizama

  • 1. Creppy EE. Update of survey, regulation and toxic effects of mycotoxins in Europe. Toxicol Lett 2002;127:19-28.

  • 2. Sweeney MJ, Dobson ADW. Mycotoxin production by Aspergillus, Fusarium and Penicillium species. Int J Food Microbiol 1998;43:141-58.

  • 3. Suneja SK, Ram GC, Wagle DS. Effects of feeding T-2 toxin on RNA, DNA and protein contents of liver and intestinal mucosa of rats. Toxicol Lett 1983;18:73-6. [Crossref] [PubMed]

  • 4. Ven Murthy MR, Radouco-Thomas S, Bharucha AD, Levesoue G, Pandian S, Radouco-Thomas C. Effects of trichothecenes (T-2 toxin) on protein synthesis in vitro by brain polysomes and messenger RNA. Prog Neuropsychopharmacol Biol Psychiatry 1985;9:251-8. [Crossref]

  • 5. Wang J, Fitzpatrick DW, Wilson JR. Effect of T-2 toxin on blood-brain barrier permeability monoamine oxidase activity and protein synthesis in rats. Food Chem Toxicol 1998;36:955-61.

  • 6. Medina A, Magan N. Comparisons of water activity and temperature impacts on growth of Fusarium langsethiae strains from northern Europe on oat-based media. Int J Food Microbiol 2010;142:365-9. [Web of Science]

  • 7. Torp M, Nirenberg HI. Fusarium langsethiae sp. nov. on cereals in Europe. Int J Food Microbiol 2004;95:247-56.

  • 8. Fournier R, Boivin P. Suivis visuel et biomoleculaire des populations de Fusarium toxinogenes et des toxines produites sur les orges de brasserie francaises au cours des recoltes 2003 a 2006 [Visual and biomolecular monitoring of Fusarium and mycotoxin content in French malting barley sampled from harvests 2003 to 2006, in French]. In: Colloque Fusariotoxines des Cereales; 11-13 Sep 2007; Arcachon, France. [displayed 19 September 2012]. Available at http:// www.symposcience.org/exl-doc/colloque/ART-00002115. pdf

  • 9. Strub C, Pocaznoi D, Lebrihi A, Fournier R, Mathieu F. Influence of barley malting operating parameters on T-2 and HT-2 toxinogenesis of Fusarium langsethiae, a worrying contaminant of malting barley in Europe. Food Addit Contam Part A Chem Anal Control Expo Risk Assess 2010;27:1247-52. [Web of Science] [Crossref]

  • 10. Logrieco A, Mule G, Moretti A, Bottalico A. Toxigenic Fusarium species and mycotoxins associated with maize ear rot in Europe. Eur J Plant Pathol 2002;108:597-609.

  • 11. Medina A, Magan N. Temperature and water activity effects on production of T-2 and HT-2 by Fusarium langsethiae strains from north European countries. Food Microbiol 2011;28:392-8. [Crossref]

  • 12. Muller H-M, Reimann J, Schumacher U, Schwadorf K. Natural occurrence of Fusarium toxins in barley harvested during five years in an area of southwest Germany. Mycopathologia 1997;137:185-92.

  • 13. Bertuzzi T, Rastelli S, Mulazzi A, Donadini G, Pietri A. Mycotoxin occurrence in beer produced in several European countries. Food Control 2011;22:2059-64. [Web of Science] [Crossref]

  • 14. Wolf-Hall CE. Mold and mycotoxin problems encountered during malting and brewing. Int J Food Microbiol 2007;119:89-94. [Web of Science]

  • 15. Boivin P, Malanda MB; Institut Francais des Boissons de la Brasserie. Inoculation by Geotrichum candidum during malting of cereals or other plants. Patent EP0826029 B1. France 1999.

  • 16. Trebstein A, Seefeldet W, Lauber U, Humpf H-U. Determination of T-2 and HT-2 toxins in cereals including oats after immunoaffinity cleanup by liquid chromatography and fluorescence detection. J Agric Food Chem 2008;56:4968-75.

  • 17. Lippolis V, Pascale M, Maragos CM, Visconti A. Improvement of detection sensitivity of T-2 and HT-2 toxins using different fluorescent labeling reagents by high-performance liquid chromatography. Talanta 2008;74:1476-83. [Crossref] [PubMed] [Web of Science]

  • 18. Pascale M, Haidukowski M, Visconti A. Determination of T-2 toxin in cereal grains by liquid chromatography with fluorescence detection after immunoaffinity column clean-up and derivatization with 1-anthroylnitrile. J Chromatogr A 2003;989:257-64.

  • 19. Trebstein A, Marschik S, Lauber U, Humpf H-U. Acetonitrile: the better extractant for the determination of T-2 and HT-2 toxin in cereals using an immunoaffinity-based cleanup? Eur Food Res Technol 2009;228:519-29. [Web of Science]

  • 20. Visconti A, Lattanzio V, Pascale M, Haidukowski M. Analysis of T-2 and HT-2 toxins in cereal grains by immunoaffinity clean-up and liquid chromatography with fluorescence detection. J Chromatogr A 2005;1075:151-8.

  • 21. Adour L, Couriol C, Amrane A, Prigent Y. Growth of Geotrichum candidum and Penicillium camembertii in liquid media in relation with the consumption of carbon and nitrogen sources and the release of ammonia and carbon dioxide. Enzyme Microbial Technol 2002;31:533-42.

  • 22. Blesa J, Meca G, Rubert J, Soriano JM, Ritieni A, Manes J. Glucose influence on the production of T-2 toxin by Fusariumsporotrichioides. Toxicon 2010;55:1157-61. [Web of Science]

  • 23. Marasas WFO, Yagen B, Sydenham E, Combrinck S, Thiel PG. Comparative yields of T-2 toxin and related trichothecenes from five toxicologically important strains of Fusariumsporotrichioides. Appl Environ Microbiol 1987;53:693-8. [PubMed]

  • 24. Ramirez Santos J, Contreras Ferrat G, Gomez Eichelmann MC. La fase estacionaria en la bacteria Escherichia coli [Stationary phase in Escherichia coli, in Spanish]. Rev Latinoam Microbiol 2005;47:92-101.

About the article

The subject of this article has partly been presented at the International Symposium “Power of Fungi and Mycotoxins in Health and Disease” held in Primošten, Croatia, from 19 to 22 October 2011

Published Online: 2013-01-16

Published in Print: 2012-12-01

Citation Information: Archives of Industrial Hygiene and Toxicology, ISSN (Print) 0004-1254, DOI: https://doi.org/10.2478/10004-1254-63-2012-2206. Export Citation

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