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Acta Parasitologica

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Volume 53, Issue 1 (Mar 2008)


The occurrence of Pneumocystis jirovecii in people from three different age groups of Warsaw (Poland) community

Elżbieta Gołąb / Małgorzata Sadkowska-Todys / Marek Szkoda / Tadeusz Dzbeński
Published Online: 2008-04-07 | DOI: https://doi.org/10.2478/s11686-008-0003-1


The three age groups of people from Warsaw: children, young adults and elderly people were examined for the prevalence of infection with Pneumocystis jirovecii. Nested PCR was used to amplify fragment of mitochondrial large subunit rRNA of the fungus in samples of oropharyngeal swabs. Nineteen (12.8%) of the 148 examined samples were positive for DNA of P. jirovecii. The samples collected from children were more often positive than the samples from young adults (p = 0.003) or from both groups of adults (p = 0.0029). Moreover, among adults (n = 99) proportion of infected women (n = 6; 12.5%) was significantly higher than men (n = 1; 2%). Results of the research confirm the high prevalence of Pneumocystis infection in children and indirectly point out to children as a possible source of infection for older people.

Keywords: Pneumocystis jirovecii; prevalence; age groups

  • [1] Bartlett M.S., Vermund S.H., Jacobs R., Durant P.J., Shaw M.M., Smith J.W., Tang X., Lu J.-J., Li B., Jin S., Lee C.-H. 1997. Detection of Pneumocystis carinii DNA in air samples: likely environmental risk to susceptible persons. Journal of Clinical Microbiology, 35, 2511–2513. Google Scholar

  • [2] Beard C.B., Carter J.L., Keely S.P., Huang L., Pieniazek N.J., Moura I.N.S., Roberts J.M., Hightower A.W., Bens M.S., Freeman A.R., Lee S., Stringer J.R., Duchin J.S., del Rio C., Rimland D., Baughman R.P., Levy D.A., Dietz V.J., Simon P., Navin T.R. 2000. Genetic variation in Pneumocystis carinii isolates from different geographic regions: implications for transmission. Emerging Infectious Diseases, 6, 265–272. http://dx.doi.org/10.3201/eid0603.000306CrossrefGoogle Scholar

  • [3] Calderon E., de la Horra C., Medrano F.J., Lopez-Suarez A., Montez-Cano M.A., Respadiaza N., Elvira-Gonzalez J., Martin-Jouan J., Bascunana A., Varela J.M. 2004. Pneumocystis jirovecii isolates with dihydropteroatensyntase mutations in patients with chronic bronchitis. European Journal of Clinical Microbiology and Infectious Diseases, 23, 545–549. DOI: 10.1007/s10096-004-1151-3. CrossrefGoogle Scholar

  • [4] Chen F., Gigliotti F., Harmsen A.G. 1993. Latency is not an inevitable outcome of infection with Pneumocystis carinii. Infection and Immunity, 61, 5406–5409. Google Scholar

  • [5] Durand-Joly I., Soula F., Chabe M., Dalle J.H., Lafitte J.J., Senechal M., Pinon A., Camus D., Dei-Cas E. 2003. Long-term colonisation with Pneumocystis jirovecii in hospital staffs: a challenge to prevent nosocomial pneumocystosis. Journal of Eukaryotic Microbiology, 50,Suppl., 614–615. (Accepted November 26, 2007) http://dx.doi.org/10.1111/j.1550-7408.2003.tb00650.xCrossrefGoogle Scholar

  • [6] Gołąb E., Sobolewska A., Matysiak E. 2002. Occurrence of Pneumocystis carinii DNA and anti-Pneumocystis antibodies in sera of infants at the age of humoral response natural degradation. Wiadomości Parazytologiczne, 48, 287–292. Google Scholar

  • [7] Gołąb E., Szkoda T., Dzbeński T.H. 2006. An evaluation of the occurrence of Pneumocystis infection through examination of pharyngeal and oral swabs using the nested PCR method. Medycyna Doświadczalna i Mikrobiologia, 58, 71–76. Google Scholar

  • [8] Helweg-Larsen J., Jensen J.S., Dohn B., Benfield T.L., Lundgren B. 2002. Detection of Pneumocystis DNA in samples from patients suspected of bacterial pneumonia — a case — control study. BMC Infectious Diseases, 2, 28. DOI: 10.1186/1471-2334-2-28. http://dx.doi.org/10.1186/1471-2334-2-28CrossrefGoogle Scholar

  • [9] Icenhour C.R., Rebholz S.L., Collins M.S. 2001. Widespread occurrence of Pneumocystis carinii in commercial rat colonies detected using targeted PCR and oral swabs. Journal of Clinical Microbiology, 3, 3437–3441. DOI: 10.1128/JCM.39.10.3437-3441.2001. http://dx.doi.org/10.1128/JCM.39.10.3437-3441.2001CrossrefGoogle Scholar

  • [10] Keely S.P., Stringerb J.R., Baughman R.P., Linke M.J., Walzer P.D., Smulian A.G. 1995. Genetic variation among Pneumocystis carinii hominis isolates in recurrent pneumocystosis. Journal of Infectious Diseases, 172, 595–598. CrossrefGoogle Scholar

  • [11] Latouche S., Rabodonirina M., Mazaras E. 1998. Pneumocystis: the ‘carrier state’: epidemiology and transmission of human pneumocystosis. FEMS Immunology and Medical Microbiology, 22, 75–80. DOI:10.1111/j.1574-695X.1998.tb01191.x. http://dx.doi.org/10.1111/j.1574-695X.1998.tb01189.xCrossrefGoogle Scholar

  • [12] Linke M.J., Rebholz S., Collins M., Tanaka R., Cushion M.T. 2003. Noninvasive method for monitoring Pneumocystis carinii pneumonia. Emerging Infectious Diseases, 9, 1613–1616. CrossrefGoogle Scholar

  • [13] Maskell N.A., Waine D.J., Lindley A., Pepperell J.C.T., Wakefield A.E., Miller R.F., Davies R.J.O. 2003. Asymptomatic carriage of Pneumocystis jirovecii in subjects undergoing bronchoscopy: a prospective study. Thorax, 58, 594–597. http://dx.doi.org/10.1136/thorax.58.7.594CrossrefGoogle Scholar

  • [14] Medrano F.J., Montes-Cano M., Conde M., de la Horra C., Respadiaza N., Gasch A., Perez-Lozano M.J., Varela J.M., Calderon E.J. 2005. Pneumocystis jirovecii in general population. Emerging Infectious Diseases, 11, 245–250. CrossrefGoogle Scholar

  • [15] Miller R.F., Ambrose H.E., Wakefield A.E. 2001. Pneumocystis carinii f.sp. hominis DNAin immunocompetent health workers in contact with patients with P. carinii pneumonia. Journal of Clinical Microbiology, 39, 3877–3882. DOI: 10.1128/JCM.39.11.3877-3882.2001. http://dx.doi.org/10.1128/JCM.39.11.3877-3882.2001CrossrefGoogle Scholar

  • [16] Nevez G., Magois E., Dukat H., Gouileux V., Jounieaux V., Totet A. 2006. Apparent absence of Pneumocystis jirovecii in healthy subject. Clinical Infectious Diseases, 42, 99–101. http://dx.doi.org/10.1086/503908CrossrefGoogle Scholar

  • [17] Nevez G.C., Totet A., Pautard J.C., Raccourt C. 2001. Pneumocystis carinii detection using nested-PCR in nasopharyngeal aspirates of immunocompetent infants with bronchiolitis. Journal of Eukaryotic Microbiology, 48,Suppl., 122–123. http://dx.doi.org/10.1111/j.1550-7408.2001.tb00479.xCrossrefGoogle Scholar

  • [18] O’Donnell W.J., Pieciak W., Chertow G.M., Sanabria J., Lahive K.C. 1998. Clearance of Pneumocystis carinii cystic in acute P. carinii pneumonia: assessment serial sputum induction. Chest, 114, 1264–1268. http://dx.doi.org/10.1378/chest.114.5.1264CrossrefGoogle Scholar

  • [19] Olsson M., Lidman C., Latouche S., Bjorkman A., Roux P., Linder E., Wahlgren M. 1998. Identification of Pneumocystis carinii f.sp. hominis gene sequences in filtered air in hospital environments. Journal of Clinical Microbiology, 36, 1737–1740. Google Scholar

  • [20] Respadiaza N., Medrano F.J., Medrano A.C., Varela J.M., de la Horra C., Montes-Cano M., Ferrer S., Wichmann I., Gargallo-Viola D., Calderon E.J. 2004. High seroprevalence of Pneumocystis infection in Spanish children. Clinical Microbiology and Infection, 10, 1029–1031. http://dx.doi.org/10.1111/j.1469-0691.2004.00974.xCrossrefGoogle Scholar

  • [21] Tamburrini E., Mencarini P., Visconti E., Zolfo M., De Luca A., Siracusano A., Ortona E., Wakefield A.E. 1996. Detection of Pneumocystis carinii DNA in blood by PCR is not of value for diagnosis of P. carinii pneumonia. Journal of Clinical Microbiology, 34, 1586–1588. Google Scholar

  • [22] Vargas S.L., Hughes W.T., Santolaya M.E., Ulloa A.V., Ponce C.A., Cabrera C.E., Cumsille F., Gigliotti F. 2001. Search for primary infection by Pneumocystis carinii in a cohort of normal, healthy infants. Clinical Infectious Diseases, 32, 855–861. http://dx.doi.org/10.1086/319340CrossrefGoogle Scholar

  • [23] Vargas S.L., Ponce C.A., Gigliotti F., Ulloa A.V., Prieto S., Munoz M.P., Hughes W.T. 2000. Transmission of Pneumocystis carinii DNA from a patient with P. carinii pneumonia to immunocompetent contact health care workers. Journal of Clinical Microbiology, 38, 1536–1538. Google Scholar

  • [24] Vargas S.L., Ponce C.A., Sanchez C.A., Ulloa A.V., Bustamante R., Juarez G. 2000. Pregnancy and asymptomatic carriage of Pneumocystis jirovecii. Emerging Infectious Diseases, 9, 605–606. Google Scholar

  • [25] Wakefield A.E., Pixley F.J., Banerji S., Sinclair K., Miller R.F., Moxon E.R., Hopkin J.M. 1990. Detection of Pneumocystis carinii with DNA amplification. Lancet, 336, 451–453. http://dx.doi.org/10.1016/0140-6736(90)92008-6CrossrefGoogle Scholar

About the article

Published Online: 2008-04-07

Published in Print: 2008-03-01

Citation Information: Acta Parasitologica, ISSN (Online) 1896-1851, ISSN (Print) 1230-2821, DOI: https://doi.org/10.2478/s11686-008-0003-1.

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© 2008 W. Stefański Institute of Parasitology, PAS. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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