Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Acta Parasitologica


IMPACT FACTOR 2017: 1.039
5-year IMPACT FACTOR: 1.121

CiteScore 2018: 1.00

SCImago Journal Rank (SJR) 2018: 0.500
Source Normalized Impact per Paper (SNIP) 2018: 0.664

More options …
Volume 53, Issue 2

Issues

Studies on the development and role of some cell-mediated immune responses in experimental toxocarosis in mice

Natalia Wnukowska / Tadeusz Dzbeński
Published Online: 2008-06-18 | DOI: https://doi.org/10.2478/s11686-008-0024-9

Abstract

The studies were undertaken to investigate the development of some cell-mediated immune responses in experimental toxocarosis in mice and to assess the influence of these responses on the course of infection. Mice were infected orally with 350 eggs of Toxocara canis and reinfected with the same dose of parasites after 8 weeks. Groups of infected animals were killed each week of the experiments to obtain spleens, livers and brains for further studies. Lymphocytes from removed spleens were analysed by flow-cytometry for CD4 and CD8 expression and cultured in vitro to measure their responses to Concanavalin A and excretory-secretory (ES) antigen of T. canis in a lymphocyte transformation test. Pieces of livers were used to prepare paraffin sections to be stained later with haematoxylin and eosin, whereas whole brains of the infected animals were examined for the presence of parasite larvae. The results of the studies showed depression of T-cell responses to ConA in early stages of infection and significant increase in the blastogenic responses to the ES antigen from week 4 following infection. The depression of T-cell responses was accompanied by lowered CD4+/CD8+ ratio resulting from increased percentages of CD8+ T cells. Histopathological examination of liver sections revealed trapping of larvae in T. canis reinfected mice. The intensity of infection as measured by larval recoveries from the brains of mice increased gradually up to the 8th week of infection, but did not show significant changes after reinfection, testifying to the development of long-lasting protective immunity during primary infection.

Keywords: Toxocara canis; CMI responses; protective immunity

  • [1] Abo-Shehada M.N., Al.-Zubaidy B.A., Herbert I.V. 1991. Acquired immunity to Toxocara canis infection in mice. Veterinary Parasitology, 38, 289–298. http://dx.doi.org/10.1016/0304-4017(91)90141-HCrossrefGoogle Scholar

  • [2] Aldawek A.M., Levkut M., Revajova V., Dvoroznakova E. 2002. Larval toxocarosis in mice: immunoreactivity after multiple high dose infections. Acta Parasitologica, 47, 249–254. Google Scholar

  • [3] De Savigny D. 1975. In vitro maintenance of Toxocara canis larvae and a simple method for the production of Toxocara ES antigen for use in serodiagnostic tests for visceral larva migrans. Journal of Parasitology, 61, 781–782. http://dx.doi.org/10.2307/3279492CrossrefGoogle Scholar

  • [4] Despommier D. 2003. Toxocariasis: clinical aspects, epidemiology, medical ecology, and molecular aspects. Clinical Microbiology Reviews, 16, 265–272. DOI: 10.1128/CMR.16.2.265-272.2003. http://dx.doi.org/10.1128/CMR.16.2.265-272.2003CrossrefGoogle Scholar

  • [5] Kayes S.G. 1984. Spleen cell responses in experimental murine toxocariasis. Journal of Parasitology, 70, 522–529. http://dx.doi.org/10.2307/3281400CrossrefGoogle Scholar

  • [6] Kayes S.G., Jones R.E., Omholt P.E. 1987. Use of bronchoalveolar lavage to compare local pulmonary immunity with the systemic immune response of Toxocara canis-infected mice. Infection and Immunity, 55, 2132–2136. Google Scholar

  • [7] Kayes S.G., Oaks J.A. 1978. Development of the granulomatous response in murine toxocariasis. American Journal of Pathology, 93, 277–294. Google Scholar

  • [8] Kayes S.G., Omholt P.E., Grieve R.B. 1985. Immune responses of CBA/J mice to graded infections with Toxocara canis. Infection and Immunity, 48, 697–703. Google Scholar

  • [9] Metwali A., Setiawan T., Blum A.M., Urban J., Elliot D.E., Hang L., Weinstock J.V. 2006. Induction of CD8+ regulatory T cells in the intestine by Heligmosomoides polygyrus infection. American Journal of Physiology. Gastrointestinal and Liver Physiology, 291, 253–259. DOI: 10.1152/ajpgi.00409.2005. http://dx.doi.org/10.1152/ajpgi.00409.2005CrossrefGoogle Scholar

  • [10] Noble A., Giorgini A., Leggat J.A. 2006. Cytokine-induced IL-10-secreting CD8 T cells represent a phenotypically distinct suppressor T cell lineage. Blood, 107, 4475–4483. DOI: 10.1182/blood-2005-10-3994. http://dx.doi.org/10.1182/blood-2005-10-3994CrossrefGoogle Scholar

  • [11] Sugane K., Oshima T. 1983. Trapping of large numbers of larvae in the livers of Toxocara canis-reinfected mice. Journal of Helminthology, 57, 95–97. http://dx.doi.org/10.1017/S0022149X00009342CrossrefGoogle Scholar

  • [12] Yamashita U., Jiang H., Inoue H., Mutoh Y., Furukawa T. 1993. Japanese Journal of Parasitology, 42, 211–219. Google Scholar

About the article

Published Online: 2008-06-18

Published in Print: 2008-06-01


Citation Information: Acta Parasitologica, Volume 53, Issue 2, Pages 205–210, ISSN (Online) 1896-1851, ISSN (Print) 1230-2821, DOI: https://doi.org/10.2478/s11686-008-0024-9.

Export Citation

© 2008 W. Stefański Institute of Parasitology, PAS. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

[1]
Ahmad A. Othman, Dalia S. Ashour, and Dareen A. Mohamed
Experimental Parasitology, 2013, Volume 134, Number 1, Page 76

Comments (0)

Please log in or register to comment.
Log in