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Volume 58, Issue 1

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Molecular characterization of Trypanosoma evansi isolates from water buffaloes (Bubalus bubalis) in the Philippines

Marjo Villareal / Claro Mingala / Windell Rivera
  • Institute of Biology, College of Science, University of the Philippines, Diliman, Quezon City, 1101, Philippines
  • Molecular Protozoology Laboratory, Natural Sciences Research Institute, University of the Philippines, Diliman, Quezon City, 1101, Philippines
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Published Online: 2013-02-02 | DOI: https://doi.org/10.2478/s11686-013-0110-5

Abstract

Trypanosoma evansi infection in the Philippines is frequently reported to affect the country’s livestock, particularly, the buffaloes. To assess the prevalence and intraspecific diversity of T. evansi in the country, blood samples from water buffaloes in different geographical regions were collected during an outbreak. T. evansi was detected in all 79 animals tested using PCR targeting the RoTat 1.2 VSG gene. Sequencing of the rDNA complete internal transcribed spacer (ITS) region including the 5.8S subunit showed high similarity (99–100%) between Philippine isolates and known T. evansi isolates in Genbank. Tree construction based on the same region confirmed the close relationship between Philippine and reported Thai isolates as compared to Egyptian isolates separated by relatively small genetic distances, 47 polymorphisms, despite the clustering in four branches. Overall, the results of this study prove genetic diversity within T. evansi species despite previous reports on limited heterogeneity among isolates worldwide.

Keywords: Trypanosoma evansi; ITS region; DNA sequencing; phylogeny; water buffaloes; Philippines

  • [1] Agbo E.C., Majiwa P.A., Claassen E.J., Roos M.H. 2001. Measure of molecular diversity within the Trypanosoma brucei subspecies Trypanosoma brucei brucei and Trypanosoma brucei gambiense as revealed by genotypic characterization. Experimental Parasitology, 99, 123–131. DOI: 10.1006/expr.2001.4666. http://dx.doi.org/10.1006/expr.2001.4666CrossrefGoogle Scholar

  • [2] Amer S., Ryu O., Tada C., Fukuda Y., Inoue N., Nakai Y. 2011. Molecular identification and phylogenetic analysis of Trypanosoma evansi from dromedary camels (Camelus dromedarius) in Egypt, a pilot study. Acta Tropica, 117, 39–46. DOI: 10.1016/j.actatropica.2010.09.010. http://dx.doi.org/10.1016/j.actatropica.2010.09.010Web of ScienceCrossrefGoogle Scholar

  • [3] Antoine-Moussiaux N., Magez S., Desmecht D. 2008. Contributions of experimental mouse models to the understanding of African trypanosomiasis. Trends in Parasitology, 24, 411–418. DOI: 10.1016/j.pt.2008.05.010. http://dx.doi.org/10.1016/j.pt.2008.05.010Web of ScienceCrossrefGoogle Scholar

  • [4] Areekit S., Singhapan P., Kanjanavas P., Khuchanreontaworn S., Sriyapai T., Pakpitcharoen A., Chansiri K. 2008. Genetic diversity of Trypanosoma evansi in beef cattle based on internal transcribed spacer region. Infection, Genetics and Evolution, 8, 484–488. DOI: 10.1292/jvms.69.487. http://dx.doi.org/10.1016/j.meegid.2007.10.008Web of ScienceCrossrefGoogle Scholar

  • [5] Aymerich S., Goldenberg S. 1989. The karyotype of Trypanosoma cruzi Dm28c: comparison with other T. cruzi strains and trypanosomatids. Experimental Parasitology, 69, 107–115. DOI: 10.1016/0014-4894(89)90178-1. http://dx.doi.org/10.1016/0014-4894(89)90178-1CrossrefGoogle Scholar

  • [6] Beltrame-Botelho I.T., Gaspar-Silva D., Steindel M., Dávila A.M., Grisard E.C. 2005. Internal transcribed spacers (ITS) of Trypanosoma rangeli ribosomal DNA (rDNA): a useful marker for inter-specific differentiation. Infection, Genetics and Evolution, 5, 17–28. DOI: 10.1016/j.meegid.2004.05.007. http://dx.doi.org/10.1016/j.meegid.2004.05.007CrossrefGoogle Scholar

  • [7] Biteau N., Bringaud F., Gibson W., Truc P., Baltz T. 2000. Characterization of Trypanozoon isolates using a repeated coding sequence and microsatellite markers. Molecular and Biochemical Parasitology, 105, 187–202. DOI: 10.1016/S0166-6851(99)00171-1. http://dx.doi.org/10.1016/S0166-6851(99)00171-1CrossrefGoogle Scholar

  • [8] Cacciò S.M., Beck R., Almeida A., Bajer A., Pozio E. 2010. Identification of Giardia species and Giardia duodenalis assemblages by sequence analysis of the 5.8S rDNA gene and internal transcribed spacers. Parasitology, 137, 919–925. DOI: 10.1017/S003118200999179X. http://dx.doi.org/10.1017/S003118200999179XWeb of ScienceCrossrefGoogle Scholar

  • [9] Carmona T.M., Garrizzo J., Roschman-González A., Tejero F., Escalante A., Aso P.M. 2006. Susceptibility of different mouse strains to experimental infection with a Venezuelan isolate of Trypanosoma evansi. Journal of Protozoology Research, 16, 1–8. Google Scholar

  • [10] Dargantes A.P., Mercado R.T., Dobson R.J., Reid S.A. 2009. Estimating the impact of Trypanosoma evansi infection (surra) on buffalo population dynamics in southern Philippines using data from cross-sectional surveys. International Journal for Parasitology, 39, 1109–1114. DOI: 10.1016/j.ijpara.2009.02.012. http://dx.doi.org/10.1016/j.ijpara.2009.02.012Web of ScienceCrossrefGoogle Scholar

  • [11] Dobson R.J., Dargantes A.P., Mercado R.T., Reid S.A. 2009. Models for Trypanosoma evansi (surra), its control and economic impact on small-hold livestock owners in the Philippines. International Journal for Parasitology, 39, 1115–1123. DOI: 10.1016/j.ijpara.2009.02.013. http://dx.doi.org/10.1016/j.ijpara.2009.02.013Web of ScienceCrossrefGoogle Scholar

  • [12] Gibson W. 2002. Epidemiology and diagnosis of African trypanosomiasis using DNA probes. Transactions of the Royal Society of Tropical Medicine and Hygiene, Suppl. 1, 141–143. CrossrefGoogle Scholar

  • [13] Guindon S., Gascuel O. 2003. A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Systematic Biology, 52, 696–704. DOI: 10.1080/10635150390235520. http://dx.doi.org/10.1080/10635150390235520CrossrefGoogle Scholar

  • [14] Hall T.A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41, 95–98. Google Scholar

  • [15] Hasegawa M., Kishino H., Yano T. 1985. Dating the human-ape splitting by a molecular clock of mitochondrial DNA. Journal of Molecular Evolution, 22, 160–174. DOI: 10.1007/BF02101694. http://dx.doi.org/10.1007/BF02101694CrossrefGoogle Scholar

  • [16] Herrera H.M., Norek A., Freitas T.P., Rademaker V., Fernandes O., Jansen A.M. 2005. Domestic and wild mammals infection by Trypanosoma evansi in a pristine area of the Brazilian Pantanal region. Parasitology Research, 96, 121–126. DOI: 10.1007/s00436-005-1334-6. http://dx.doi.org/10.1007/s00436-005-1334-6CrossrefGoogle Scholar

  • [17] Homan W.L., Limper L., Verlaan M., Borst A., Vercammen M., van Knapen F. 1997. Comparison of the internal transcribed spacer, ITS1, from Toxoplasma gondii isolates and Neospora caninum. Parasitology Research, 83, 285–289. DOI: 10.1007/s004360050248. http://dx.doi.org/10.1007/s004360050248CrossrefGoogle Scholar

  • [18] Konnai S., Mekata H., Mingala C.N., Abes N.S., Gutierrez C.A., Herrera J.R.V., Dargantes A.P., Witola W.H., Cruz L.C., Inoue N., Onuma M., Ohashi K. 2009. Development and application of a quantitative real-time PCR for the diagnosis of surra in water buffaloes. Infection, Genetics and Evolution, 9, 449–452. DOI: 10.1016/j.meegid.2009.01.006. http://dx.doi.org/10.1016/j.meegid.2009.01.006CrossrefWeb of ScienceGoogle Scholar

  • [19] Khuchareontaworn S., Singhaphan P., Viseshakul N., Chansiri K. 2007. Genetic diversity of Trypanosoma evansi in buffalo based on internal transcribed spacer (ITS) regions. Journal of Veterinary Medical Science, 69, 487–493. DOI: 10.1292/jvms.69.487. http://dx.doi.org/10.1292/jvms.69.487Web of ScienceCrossrefGoogle Scholar

  • [20] Lanave C., Preparata G., Saccone C., Serio G. 1984. A new method for calculating evolutionary substitution rates. Journal of Molecular Evolution, 20, 86–93. DOI: 10.1007/BF02101990. http://dx.doi.org/10.1007/BF02101990CrossrefPubMedGoogle Scholar

  • [21] Lollis L., Gerhold R., McDougald L., Beckstead R. 2011. Molecular characterization of Histomonas meleagridis and other parabasalids in the United States using the 5.8S, ITS-1, and ITS-2 rRNA regions. Journal of Parasitology, 97, 610–615. DOI: http://dx.doi.org/10.1645/GE-2648.1. http://dx.doi.org/10.1645/GE-2648.1Web of ScienceGoogle Scholar

  • [22] Luckins A.G. 1988. Trypanosoma evansi in Asia. Trends in Parasitology, 4, 137–142. DOI: 10.1016/0169-4758(88)90188-3. CrossrefGoogle Scholar

  • [23] Lun Z.-R., Li A.-X., Chen X.-G., Lu L.-X., Zhu X.-Q. 2004. Molecular profiles of Trypanosoma brucei, T. evansi, and T. equiperdum stocks revealed by the random amplified polymorphic DNA method. Parasitology Research, 92, 335–340. DOI: 10.1007/s00436-003-1054-8. http://dx.doi.org/10.1007/s00436-003-1054-8CrossrefGoogle Scholar

  • [24] Majiwa P.A., Webster P. 1987. A repetitive deoxyribonucleic acid sequence distinguishes Trypanosoma simiae from T. congolense. Parasitology, 95, 543–558. DOI: 10.1017/S0031182000057978. http://dx.doi.org/10.1017/S0031182000057978CrossrefGoogle Scholar

  • [25] Manuel M.F. 1998. Sporadic outbreaks of surra in the Philippines and its economic impact. Journal of Protozoology Research, 8, 131–138. Google Scholar

  • [26] Masiga D.K., Ndung’u K., Tweedie A., Tait A., Turner C.M. 2006. Trypanosoma evansi: genetic variability detected using amplified restriction fragment length polymorphism (AFLP) and random amplified polymorphic DNA (RAPD) analysis of Kenyan isolates. Experimental Parasitology, 114, 147–153. DOI: 10.1016/j.exppara.2006.03.002. http://dx.doi.org/10.1016/j.exppara.2006.03.002CrossrefGoogle Scholar

  • [27] Mekata H., Konnai S., Witola W.H., Inoue N., Onuma M., Ohashi K. 2009. Molecular detection of trypanosomes in cattle in South America and genetic diversity of Trypanosoma evansi based on expression-site-associated gene 6. Infection, Genetics and Evolution, 9, 1301–1305. DOI:10.1016/j.meegid.2009.07.009. http://dx.doi.org/10.1016/j.meegid.2009.07.009CrossrefWeb of ScienceGoogle Scholar

  • [28] Nijru Z.K., Constantine C.C., Gitonga P.K., Thompson R.C., Reid S.A. 2007. Genetic variability of Trypanosoma evansi isolates detected by inter-simple sequence repeat anchored-PCR and microsatellite. Veterinary Parasitology, 147, 51–60. DOI: 10.1016/j.vetpar.2007.03.010. http://dx.doi.org/10.1016/j.vetpar.2007.03.010CrossrefWeb of ScienceGoogle Scholar

  • [29] Page R.D. 1996. TreeView: an application to display phylogenetic trees on personal computers. Computer Applications in the Biosciences, 12, 357–358. DOI: 10.1093/bioinformatics/12.4.357. CrossrefGoogle Scholar

  • [30] Perrone T.M., Gonzatti M.I., Villamizar G., Escalante A., Aso P.M. 2009. Molecular profiles of Venezuelan isolates of Trypanosoma sp. by random amplified polymorphic DNA method. Veterinary Parasitology, 161, 194–200. DOI: 10.1016/j.vetpar.2009.01.034. http://dx.doi.org/10.1016/j.vetpar.2009.01.034CrossrefWeb of ScienceGoogle Scholar

  • [31] Reid S.A. 2002. Trypanosoma evansi control and containment in Australasia. Trends in Parasitology, 18, 219–224. DOI: 10.1016/S1471-4922(02)02250-X. http://dx.doi.org/10.1016/S1471-4922(02)02250-XCrossrefGoogle Scholar

  • [32] Santos S.S., Cupolillo E., Junqueira A., Coura J.R., Jansen A., Sturm N.R., Campbell D.A., Fernandes O. 2002. The genetic diversity of Brazilian Trypanosoma cruzi isolates and the phylogenetic positioning of zymodeme 3, based on the internal transcribed spacer of the ribosomal gene. Annals of Tropical Medicine and Parasitology, 96, 755–764. DOI: http://dx.doi.org/10.1179/000349802125002301. http://dx.doi.org/10.1179/000349802125002301Google Scholar

  • [33] Swofford D.L. 2002. PAUP* 4.0: Phylogenetic analysis using parsimony (*and other methods). Sinauer Associates, Sunderland, Massachusetts. Google Scholar

  • [34] Tamura K., Nei M. 1993. Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Molecular Biology and Evolution, 10, 512–526. Google Scholar

  • [35] Tian Z., Liu G., Xie J., Shen H., Zhang L., Zhang P., Luo J. 2011. The internal transcribed spacer 1 (ITS-1), a controversial marker for the genetic diversity of Trypanosoma evansi. Experimental Parasitology, 129, 303–306. DOI: 10.1016/j.exppara.2011.08.006. http://dx.doi.org/10.1016/j.exppara.2011.08.006Web of ScienceCrossrefGoogle Scholar

  • [36] Uche U.E., Jones T.W., Boid R. 1992. Antibody patterns in rabbits showing different levels of susceptibility to an experimental Trypanosoma evansi infection. Acta Tropica, 52, 139–147. DOI:10.1016/0001-706X(92)90030-2. http://dx.doi.org/10.1016/0001-706X(92)90030-2CrossrefGoogle Scholar

  • [37] Venus E.B., Dumag P.U. 1967. Incidence of surra infection among carabaos examined in Nueva Ecija and Pampanga. Philippine Journal of Animal Industry, 22, 177–180. Google Scholar

  • [38] Walsh P.S., D.A. Metzger, Higuchi R. 1991. Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. Biotechniques, 10, 506–513. Google Scholar

  • [39] Witola W.H., Sarataphan N., Inoue N., Ohashi K., Onuma M. 2005. Genetic variability in ESAG6 genes among Trypanosoma evansi isolates and in comparison to other Trypanozoon members. Acta Tropica, 93, 63–73. DOI: 10.1016/j.actatropica.2004.09.006. http://dx.doi.org/10.1016/j.actatropica.2004.09.006Google Scholar

  • [40] Yutuc L. 1935. Observations on the occurrence of surra in Laguna province and its relation to the 1933 outbreak among college of agricultural animals. Philippine Agriculturist, 24, 104–125. Google Scholar

About the article

Published Online: 2013-02-02

Published in Print: 2013-03-01


Citation Information: Acta Parasitologica, Volume 58, Issue 1, Pages 6–12, ISSN (Online) 1896-1851, ISSN (Print) 1230-2821, DOI: https://doi.org/10.2478/s11686-013-0110-5.

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© 2013 W. Stefański Institute of Parasitology, PAS. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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