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Acta Parasitologica

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Volume 58, Issue 3 (Sep 2013)

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Susceptibility of Acanthamoeba to multipurpose lens-cleaning solutions

Ana Aguiar
  • Departamento de Microbiologia, Imunologia e Parasitologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil
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/ Caroline Oliveira Silveira
  • Departamento de Microbiologia, Imunologia e Parasitologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil
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/ Mari Todero Winck
  • Departamento de Microbiologia, Imunologia e Parasitologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil
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/ Marilise Rott
  • Departamento de Microbiologia, Imunologia e Parasitologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil
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Published Online: 2013-08-29 | DOI: https://doi.org/10.2478/s11686-013-0143-9

Abstract

The present study investigated the susceptibility of Acanthamoeba spp. trophozoites to two multipurpose systems for cleaning and maintenance of contact lenses. Three strains of trophozoites from the ATCC (A. castellani T4, A. castellani Neff, and A. polyphaga) and two Acanthamoeba isolates obtained from swimming pools (PT5 and PO1) were placed in monoxenic culture. To test their survival in cleaning solutions for contact lenses, the trophozoites were exposed for 4 and 24 h to two multipurpose solutions (A and B), and were then inoculated into a new monoxenic culture. Amoebic growth on the plates was observed after 72 h of incubation. Trophozoites from all three ATCC strains and one isolate from a swimming pool (PO1) grew in all plates after 4 h of exposure to solutions A and B. After 24 h, the ATCC strains and the PO1 isolate showed growth in most of the plates treated. Only the PT5 isolate showed susceptibility to both solutions over the time intervals tested. The two solutions were not completely effective against most strains and isolates over the time intervals tested. These results are important, since species of Acanthamoeba are widely distributed in the environment and are potential agents of eye pathologies.

Keywords: Acanthamoeba; multipurpose solutions; keratitis

  • [1] Booton G.C., Visvesvara G.S., Byers T.J., Byers T.J., Kelly D.J., Fuerst P.A. 2005. Identification and distribution of Acanthamoeba species genotypes associated with nonkeratitis infections. Journal of Clinical Microbiology, 43, 1689–1693. DOI: 10.1128/JCM.43.4.1689-1693.2005. http://dx.doi.org/10.1128/JCM.43.4.1689-1693.2005CrossrefGoogle Scholar

  • [2] BRASIL, 2011. Agência Nacional de Vigilancia Sanitária. Serviçp de Registro de Produtos para Saúde [ANVISA web site]. Available in: www.anvisa.gov.br. Accessed November 7, 2010. Google Scholar

  • [3] Bryan K., Bugant J., Chang T., Chen S., Rosenberg J., Hammond R., McConnell K., Sanderson R., Nakata M., Wakida C., Austin C., Bestudik J., Bordson M.G., Conover C., Granzow L., Rea V., Chu A., Luckman E., Signs K., Harper J., Damrow T., Mosher E., Kruger K., Saheli E., Cassidy M., Hatch J., Weltman A., Garcia Rivera E.J., Garcia Y., Kainer M.A., Archer J., Joslin C., Cernoch P., Jones D., Hamill M., Matoba A., Pflugfelder S., Wilhelmus K., Beavers S., Chen T., Christian K., Cooper M., Dufficy D., Gershman M., Glenshaw M., Hall A., Holzbauer S., Huang A., Langer A., Moore Z., Patel A.S., Carpenter L.R., Schaffzin J., Su J., Trevino I., Weiser T., Wiersma P., Lorick S., Verani J.R. Acanthamoeba keratitis multiple states, 2005–2007 MMWR [CDC web site]. Available in: http://www.cdc.gov/mmwr/. Accessed October 20, 2010. Google Scholar

  • [4] Carlesso A.M., Artuso G.L., Simonetti A.M., Rott M.B. 2007. Isolation and identification of potentially pathogenic free-living amoebae in samples from environments in a public hospital in the city of Porto Alegre, Rio Grande do Sul. Revista da Sociedade Brasileira de Medicina Tropical, 40, 316–320. DOI: 10.1590/S0037-86822007000300013. http://dx.doi.org/10.1590/S0037-86822007000300013Web of ScienceCrossrefGoogle Scholar

  • [5] Caumo K., Frasson A.P., Pens C.J., Panatieri L.F., Frazzon A.P., Rott M.B. 2009. Potentially pathogenic Acanthamoeba in swimming pools: a survey in the southern Brazilian city of Porto Alegre. Annals of Tropical Medicine and Parasitology, 103, 477–485. DOI: 10.1179/136485909X451825. http://dx.doi.org/10.1179/136485909X451825Web of ScienceCrossrefGoogle Scholar

  • [6] Caumo K., Rott MB. 2011. Acanthamoeba T3, T4 and T5 in swimming-pool waters from Southern Brazil. Acta Tropica, 117, 233–235. DOI: 10.1016/j.actatropica.2010.12.008. http://dx.doi.org/10.1016/j.actatropica.2010.12.008Web of ScienceCrossrefGoogle Scholar

  • [7] Cohen E.J., Buchanan H.W., Laughrea P.A., Adams C.P., Galentine P.G., Visvesvara G.S., Folberg R., Arentsen J.J., Laibson P.R. 1985. Diagnosis and management of Acanthamoeba keratitis. American Journal of Ophthalmology, 100, 389–395. Google Scholar

  • [8] Corsaro D., Venditti D. 2010. Phylogenetic evidence for a new genotype of Acanthamoeba (Amoebozoa, Acanthamoebida). Parasitology Research, 107, 233–238. DOI: 10.1007/s00436-010-1870-6. http://dx.doi.org/10.1007/s00436-010-1870-6CrossrefWeb of ScienceGoogle Scholar

  • [9] De Carli G.A., Moura H. 2007. Amebas de Vida Livre. In: De Carli GA. 2nd Ed. Parasitologia Clínica, Seleção de Métodos e Técnicas de Laboratório para o Diagnóstico das Parasitoses Humanas. Atheneu, Rio de Janeiro, 417–427. Google Scholar

  • [10] De Jonckheere J.F. 2003. Epidemiological typing of Acanthamoeba strains isolated from keratitis cases in Belgium. Bull Societ Belge Ophtalmology, 287, 27–33. Google Scholar

  • [11] Gast R.J. 2001. Development of Acanthamoeba-specific reverse dotblot and the discovery of a new ribotype. Journal of Eukaryotic Microbiology, 48, 609–615. http://dx.doi.org/10.1111/j.1550-7408.2001.tb00199.xGoogle Scholar

  • [12] Hiti K., Walochnik J., Haller-Schober E.M., Faschinger C., Aspöck H. 2002. Viability of Acanthamoeba after exposure to a multipurpose disinfecting contact lens solution and two hydrogen peroxide systems. British Journal of Ophthalmology, 86, 144–146. DOI: 10.1136/bjo.86.2.144. http://dx.doi.org/10.1136/bjo.86.2.144CrossrefGoogle Scholar

  • [13] Iovieno A., Oechsler R.A., Ledee D.R., Miller D., Alfonso E.C. 2010. Drug-resistant severe Acanthamoeba keratitis caused by rare T5 Acanthamoeba genotype. Eye & Contact Lens, 36, 183–184. DOI: 10.1097/ICL.0b013e3181da2350. http://dx.doi.org/10.1097/ICL.0b013e3181da2350CrossrefWeb of ScienceGoogle Scholar

  • [14] Khan N.A., Jarrol E.L., Paget T.A. 2002. Molecular and physiological differentiation between pathogenic and nonpathogenic Acanthamoeba. Current Microbiology, 45, 197–202. DOI: 10.1007/s00284-001-0108-3. http://dx.doi.org/10.1007/s00284-001-0108-3CrossrefGoogle Scholar

  • [15] Lipener C., Ray C.B. 2008. Sistemas atuais de cuidado e manutenção de lentes de contato. Arquivos Brasileiros de Oftalmologia, 71, 9–13. http://dx.doi.org/10.1590/S0004-27492008000700003Google Scholar

  • [16] Maghsood A.H., Sissons J., Rezaian M., Nolder D., Warhurst D., Khan N.A. 2005. Acanthamoeba genotypes T4 from the UK and Iran and isolation of the T2 genotype from clinical isolates. Journal of Medical Microbiology, 74, 755–759. DOI: 10.1099/jmm.0.45970-0. http://dx.doi.org/10.1099/jmm.0.45970-0CrossrefGoogle Scholar

  • [17] Marciano-Cabral, Cabral G. 2003. Acanthamoeba spp. as agents of diseases in humans. Clinical Microbiology Reviews, 16, 273–307. DOI: 10.1128/CMR.16.2.273-307.2003. http://dx.doi.org/10.1128/CMR.16.2.273-307.2003CrossrefGoogle Scholar

  • [18] McClellan K., Coster D.J. 1987. Acanthamoebic keratitis diagnosed by paracentesis and biopsy and treated with propamidine. British Journal of Ophthalmology, 71, 734–736. http://dx.doi.org/10.1136/bjo.71.10.734Google Scholar

  • [19] Molmeret M., Horn M., Wagner M., Santic M., Abu Kwaik Y. 2005. Amoebae as training grounds for intracellular bacterials pathogens. Applied and Environmental Microbiology, 71, 20–28. DOI: 10.1128/AEM.71.1.20-28.2005. http://dx.doi.org/10.1128/AEM.71.1.20-28.2005CrossrefGoogle Scholar

  • [20] Morlet N., Duguid G., Radford C., Matheson M., Dart J. 1997. Incidence of Acanthamoeba keratitis associated with contact lens wear. Lancet, 350, 414–416. http://dx.doi.org/10.1016/S0140-6736(05)64138-4Google Scholar

  • [21] Patel A., Hammersmith K. 2008. Contact lens-related microbial keratitis: recent outbreaks. Current Opinion in Ophthalmology, 19, 302–306. DOI: 10.1097/ICU.0b013e3283045e74. http://dx.doi.org/10.1097/ICU.0b013e3283045e74CrossrefWeb of ScienceGoogle Scholar

  • [22] Pens C.J., da Costa M., Fadanelli C., Caumo K., Rott M. 2008. Acanthamoeba spp. and bacterial contamination in contact lens storage cases and the relationship to user profiles. Parasitology Research, 103, 1241–1245. DOI: 10.1007/s00436-008-1120-3. http://dx.doi.org/10.1007/s00436-008-1120-3Web of ScienceCrossrefGoogle Scholar

  • [23] Radfort C.F., Minassian D.C., Dart J.K.G. 2002. Acanthamoeba keratitis in England and Wales: incidence, outcome, and risks factors. Britsh Journal of Ophthalmology, 86, 536–542. DOI: 10.1136/bjo.86.5.536. http://dx.doi.org/10.1136/bjo.86.5.536CrossrefGoogle Scholar

  • [24] Schaumberg D.A., Snow K.K., Dana M.R. 1998. The epidemic of Acanthamoeba keratitis: where do we stand? Cornea, 17, 3–10. http://dx.doi.org/10.1097/00003226-199801000-00001Google Scholar

  • [25] Shoff M., Rogerson A., Schatz S., Seal D. 2007.Variable responses of Acanthamoeba strains to three multipurpose lens cleaning solutions. Investigative Ophthalmology & Visual Science, 84, 202–207. DOI: 10.1097/OPX.0b013e3180339f81. CrossrefGoogle Scholar

  • [26] Shoff M.E., Joslin C.E., Tu E.Y., Kubatko L., Fuerst P.A. 2008. Efficacy of contact lens systems against recent clinical and tap water Acanthamoeba isolates. Cornea, 27, 713–719. DOI: 10.1097/QAI.0b013e31815e7251. CrossrefGoogle Scholar

  • [27] Spanakos G., Tzanetou K., Miltsakakis D., Patsoula E., Malamou-Lada E., Vakalis N.C. 2006. Genotyping of pathogenic Acanthamoeba isolated from clinical samples in Greece — report of a clinical isolate presenting T5 genotype. Parasitology International, 55, 147–149. DOI: 10.1016/j.parint.2005.12.001. http://dx.doi.org/10.1016/j.parint.2005.12.001CrossrefGoogle Scholar

  • [28] Stothard D.R., Schroeder-Diedrich J.M., Awwad M.H., Gast R.J., Ledee D.R., Rodriguez-Zaragoza S., Dean C.L., Fuerst P.A., Byers T.J. 1998. The evolutionary history of the genus Acanthamoeba and identification of eight new 18S rRNA gene sequence types. Journal of Eukaryotic Microbiology, 45, 45–54. DOI: 10.1111/j.1550-7408.1998.tb05068.x. http://dx.doi.org/10.1111/j.1550-7408.1998.tb05068.xCrossrefGoogle Scholar

  • [29] Theodore F.H., Jakobiec F.A., Juechter K.B., Ma P., Troutman R.C., Panq P.M., Iwamoto T. 1985. The diagnostic value of a ring infiltrate in acanthamoebic keratitis. Ophthalmology, 2, 1471–1479. CrossrefGoogle Scholar

  • [30] Walochnick J., Obwaller A., Aspock H. 2000. Correlations between morphological, molecular, biological and physiological characteristics in clinical and nonclinical isolates of Acanthamoeba spp. Applied and Environmental Microbiology, 66, 4408–4413. DOI: 10.1128/AEM.66.10.4408-4413.2000. http://dx.doi.org/10.1128/AEM.66.10.4408-4413.2000CrossrefGoogle Scholar

  • [31] Yu H.S., Kong H.H., Kim S.Y., Hahn Y.H., Hahn T.W., Chung D.I. 2004. Laboratory investigation of Acanthamoeba lugdunensis from patients with keratitis. Investigative Ophthalmology & Visual Science, 45, 1418–1426. DOI: 10.1167/iovs.03-0433. http://dx.doi.org/10.1167/iovs.03-0433CrossrefGoogle Scholar

  • [32] Zheng X., Uno T., Goto T., Zhang W., Hill J.H. 2004. Pathogenic Acanthamoeba Induces Apoptosis of Human Corneal Epithelial cells. Japanese Journal of Ophthalmology, 48, 23–29. DOI: 10.1007/s10384-003-0018-y. http://dx.doi.org/10.1007/s10384-003-0018-yCrossrefGoogle Scholar

About the article

Published Online: 2013-08-29

Published in Print: 2013-09-01


Citation Information: Acta Parasitologica, ISSN (Online) 1896-1851, ISSN (Print) 1230-2821, DOI: https://doi.org/10.2478/s11686-013-0143-9.

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© 2013 W. Stefański Institute of Parasitology, PAS. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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