Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Acta Parasitologica

4 Issues per year


IMPACT FACTOR 2016: 1.160
5-year IMPACT FACTOR: 1.185

CiteScore 2016: 1.24

SCImago Journal Rank (SJR) 2016: 0.532
Source Normalized Impact per Paper (SNIP) 2016: 0.721

Online
ISSN
1896-1851
See all formats and pricing
More options …
Volume 58, Issue 4 (Dec 2013)

Issues

Fasciola hepatica — the pilot study of in vitro assessing immune response against native and recombinant antigens of the fluke

Piotr Bąska
  • Division of Pharmacology and Toxicology, Department of Preclinical Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences — SGGW, 8 Ciszewskiego Street, 02-786, Warsaw, Poland
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Anna Zawistowska-Deniziak
  • Witold Stefański Institute of Parasitology, Polish Academy of Sciences, 51/55 Twarda Street, 00-818, Warsaw, Poland
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Anna Zdziarska
  • Division of Parasitology and Parasitic Diseases, Department of Preclinical Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences — SGGW, 8 Ciszewskiego Street, 02-786, Warsaw, Poland
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Katarzyna Wasyl
  • Division of Parasitology and Parasitic Diseases, Department of Preclinical Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences — SGGW, 8 Ciszewskiego Street, 02-786, Warsaw, Poland
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Marcin Wiśniewski
  • Division of Parasitology and Parasitic Diseases, Department of Preclinical Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences — SGGW, 8 Ciszewskiego Street, 02-786, Warsaw, Poland
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Anna Cywińska
  • Department of Pathology and Veterinary Diagnostics, Faculty of Veterinary Medicine, Warsaw University of Life Sciences — SGGW, 159C Nowoursynowska Street, 02-776, Warsaw, Poland
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Maciej Klockiewicz
  • Division of Parasitology and Parasitic Diseases, Department of Preclinical Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences — SGGW, 8 Ciszewskiego Street, 02-786, Warsaw, Poland
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Kamil Januszkiewicz
  • Witold Stefański Institute of Parasitology, Polish Academy of Sciences, 51/55 Twarda Street, 00-818, Warsaw, Poland
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Halina Wędrychowicz
  • Witold Stefański Institute of Parasitology, Polish Academy of Sciences, 51/55 Twarda Street, 00-818, Warsaw, Poland
  • Division of Parasitology and Parasitic Diseases, Department of Preclinical Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences — SGGW, 8 Ciszewskiego Street, 02-786, Warsaw, Poland
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2013-12-13 | DOI: https://doi.org/10.2478/s11686-013-0163-5

Abstract

Fasciola hepatica is a liver fluke that infects 2.4 million of people and causes great economical loss in animal production. To date a 100% effective vaccine has not been developed and the disease is controlled by drug therapy. Great efforts are put into development of effective vaccine against parasite what is difficult since Fasciola spp. (like other helmints) during evolutionary process has developed sophisticated and efficient methods to evade immune response. During preliminary experiments it is convenient to use cell lines which are relatively cheap and allow for reproducible comparison of results between laboratories. We stimulated BOMA (bovine monocyte/macrophage cell line) and BOMAC (bovine macrophage cell line) with native or recombinant antigens of Fasciola hepatica and assessed IFN-γ, IL-4 and TNF-α level upon stimulation. We observed diminished secretion of proinflammatory TNF-α in LPS activated BOMA cells stimulated with Excretory/Secretory products of adult fluke (Fh-ES). We also observed greater changes in gene expression in LPS activated BOMA cells than in non activated BOMA cells upon stimulation using Fh-ES. The results show possibility of using cell lines for in vitro research of bovine immune response against liver fluke, although this model still requires validation and further characterization.

Keywords: Fasciola hepatica; antigens; immune response

  • [1] Aida Y., Pabst M.J. 1990. Removal of endotoxin from protein solutions by phase separation using Triton X-114. Journal of Immunological Methods, 14, 191–195. http://dx.doi.org/10.1016/0022-1759(90)90029-UCrossrefGoogle Scholar

  • [2] Bąska P., Norbury L.J., Wiśniewski M., Januszkiewicz K., Wędrychowicz H. 2013a. Excretory/secretory products of Fasciola hepatica but not recombinant phosphoglycerate kinase induce death of human hepatocyte cells. Acta Parasitologica, 58, 215–217. DOI: 10.2478/s11686-013-0126-x. http://dx.doi.org/10.2478/s11686-013-0126-xCrossrefWeb of ScienceGoogle Scholar

  • [3] Bąska P., Wiśniewski M., Krzyżowska M., Długosz E., Zygner W., Górski P., Wędrychowicz H. 2013b. Molecular cloning and characterisation of in vitro immune response against astacinlike metalloprotease Ace-MTP-2 from Ancylostoma ceylanicum. Experimental Parasitology, 133, 472–482. DOI: 10.1016/j.exppara.2013.01.006. http://dx.doi.org/10.1016/j.exppara.2013.01.006Web of ScienceCrossrefGoogle Scholar

  • [4] Clery D.G., Mulcahy G 1998. Lymphocyte and cytokine responses of young cattle during primary infection with Fasciola hepatica. Research in Veterinary Science, 65,169–171. http://dx.doi.org/10.1016/S0034-5288(98)90171-0CrossrefGoogle Scholar

  • [5] De Maere V., Vercauteren I., Geldhof P., Gevaert K., Vercruysse J., Claerebout E. 2005. Molecular analysis of astacin-like metalloproteases of Ostertagia ostertagi. Parasitology, 130, 89–98. http://dx.doi.org/10.1017/S0031182004006274CrossrefGoogle Scholar

  • [6] Donnelly S., O’Neill S.M., Sekiya M., Mulcahy G., Dalton J.P. 2005. Thioredoxin peroxidase secreted by Fasciola hepatica induces the alternative activation of macrophages. Infection and Immunity, 73, 166–173. http://dx.doi.org/10.1128/IAI.73.1.166-173.2005CrossrefGoogle Scholar

  • [7] Donofrio G., Cavirani S., van Santen V., Flammini C.F. 2005. Potential secondary pathogenic role for bovine herpesvirus 4. Journal of Clinical Microbiology, 43, 3421–3426. http://dx.doi.org/10.1128/JCM.43.7.3421-3426.2005CrossrefGoogle Scholar

  • [8] Falcón C., Carranza F., Martínez F.F., Knubel C.P., Masih D.T., Motrán C.C., Cervi L. 2010. Excretory-secretory products (ESP) from Fasciola hepatica induce tolerogenic properties in myeloid dendritic cells. Veterinary Immunology Immunopathology, 15, 36–46. DOI: 10.1016/j.vetimm.2010.04.007. http://dx.doi.org/10.1016/j.vetimm.2010.04.007Web of ScienceCrossrefGoogle Scholar

  • [9] Flynn R.J., Mulcahy G. 2008. Possible role for Toll-like receptors in interaction of Fasciola hepatica excretory/secretory products with bovine macrophages. Infection and Immunity, 76, 678–684. http://dx.doi.org/10.1128/IAI.00732-07CrossrefWeb of ScienceGoogle Scholar

  • [10] Guasconi L., Serradell M.C., Garro A.P., Iacobelli L., Masih D.T. 2011. C-type lectins on macrophages participate in the immunomodulatory response to Fasciola hepatica products. Immunology, 133, 386–396. DOI: 10.1111/j.1365-2567.2011.03449.x. http://dx.doi.org/10.1111/j.1365-2567.2011.03449.xCrossrefWeb of ScienceGoogle Scholar

  • [11] Guasconi L., Serradell M.C., Masih D.T. 2012. Fasciola hepatica products induce apoptosis of peritoneal macrophages. Veterinary Immunology and Immunopathology, 148, 359–363. DOI: 10.1016/j.vetimm.2012.06.022. http://dx.doi.org/10.1016/j.vetimm.2012.06.022Web of ScienceCrossrefGoogle Scholar

  • [12] Jaros S., Jaros D., Wesolowska A., Zygner W., Wedrychowicz H. 2010. Blocking Fasciola hepatica’s energy metabolism — a pilot study of vaccine potential of a novel gene — phosphoglycerate kinase. Veterinary Parasitology, 172, 229–237. DOI: 10.1016/j.vetpar.2010.05.008. http://dx.doi.org/10.1016/j.vetpar.2010.05.008Web of ScienceCrossrefGoogle Scholar

  • [13] Jolly A., Colavecchia S.B., Fernández B., Fernández E., Mundo S.L. 2011. Antibodies Induced by Lipoarabinomannan in Bovines: Characterization and Effects on the Interaction between Mycobacterium Avium Subsp. paratuberculosis and Macrophages In Vitro. Veterinary Medicine International, 258479. DOI: 10.4061/2011/258479. CrossrefGoogle Scholar

  • [14] Lane G. 1998. Anthelmintic resistance. Veterinary Record, 143, 232. Google Scholar

  • [15] Loukas A., Bethony J.M., Mendez S., Fujiwara R.T., Goud G.N., Ranjit N., Zhan B., Jones K., Bottazzi M.E., Hotez P.J. 2005. Vaccination with recombinant aspartic hemoglobinase reduces parasite load and blood loss after hookworm infection in dogs. PLoS Medicine. 2, e295. http://dx.doi.org/10.1371/journal.pmed.0020295CrossrefGoogle Scholar

  • [16] McManus D.P., Dalton J.P. 2006. Vaccines against the zoonotic trematodes Schistosoma japonicum, Fasciola hepatica and Fasciola gigantica. Parasitology, 133 Suppl., 43–61. http://dx.doi.org/10.1017/S0031182006001806CrossrefGoogle Scholar

  • [17] McVeigh P., Maule A.G., Dalton J.P., Robinson M.W. 2012. Fasciola hepatica virulence-associated cysteine peptidases: a systems biology perspective. Microbes and Infection, 14, 301–310. DOI: 10.1016/j.micinf.2011.11.012. http://dx.doi.org/10.1016/j.micinf.2011.11.012Web of ScienceCrossrefGoogle Scholar

  • [18] Moll L., Gaasenbeek C.P.H., Vellema P., Borgsteede H.H.M. 2000. Resistance of Fasciola hepatica against triclabendazole in cattle and sheep in The Netherlands. Veterinary Parasitology, 91, 153–158. http://dx.doi.org/10.1016/S0304-4017(00)00267-3CrossrefGoogle Scholar

  • [19] Norbury L.J., Beckham S., Pike R.N., Grams R., Spithill T.W., Fecondo J.V., Smooker P.M. 2011 Adult and juvenile Fasciola cathepsin L proteases: different enzymes for different roles. Biochimie, 93, 604–611. DOI: 10.1016/j.biochi.2010.12.004. http://dx.doi.org/10.1016/j.biochi.2010.12.004Web of ScienceCrossrefGoogle Scholar

  • [20] Norbury L.J., Januszkiewicz K., Smooker P. M. 2012. Fasciola: Parasite Biology, Disease and Control. In: (Ed. G.S. Erzinger) Parasites: Ecology, Diseases and Management. Nova Science Publishers, New York, 103–166. Google Scholar

  • [21] Overend D.J, Bowen F.L. 1995. Resistance of Fasciola hepatica to triclabendazole. Australian Veterinary Journal, 72, 275–276. http://dx.doi.org/10.1111/j.1751-0813.1995.tb03546.xCrossrefGoogle Scholar

  • [22] Pearson M.S., Bethony J.M., Pickering D.A., de Oliveira L.M., Jariwala A., Santiago H., Miles A.P., Zhan B., Jiang D., Ranjit N., Mulvenna J., Tribolet L., Plieskatt J., Smith T., Bottazzi M.E., Jones K., Keegan B., Hotez P.J., Loukas A. 2009. An enzymatically inactivated hemoglobinase from Necator americanus induces neutralizing antibodies against multiple hookworm species and protects dogs against heterologous hookworm infection. FASEB Journal, 23, 3007–3019. DOI: 10.1096/fj.09-131433. http://dx.doi.org/10.1096/fj.09-131433Web of ScienceCrossrefGoogle Scholar

  • [23] Pearson M.S., Pickering D.A., Tribolet L., Cooper L., Mulvenna J., Oliveira L.M., Bethony J.M,. Hotez P.J., Loukas A. 2010. Neutralizing antibodies to the hookworm hemoglobinase Na-APR-1: implications for a multivalent vaccine against hookworm infection and schistosomiasis. Journal of Infectious Diseases, 15,201(10), 1561–1569. DOI: 10.1086/651953. http://dx.doi.org/10.1086/651953CrossrefWeb of ScienceGoogle Scholar

  • [24] Ranjit N., Zhan B., Hamilton B., Stenzel D., Lowther J., Pearson M., Gorman J., Hotez P., Loukas A. 2009. Proteolytic degradation of hemoglobin in the intestine of the human hookworm Necator americanus. Journal of Infectious Diseases, 199, 904–912. http://dx.doi.org/10.1086/597048Web of ScienceCrossrefGoogle Scholar

  • [25] Rola M., Materniak M., Pluta A., Kuzmak J. 2011. DNA microarray gene expression profile of bovine macrophages cell line (BoMac) after infection with Bovine Immunodeficiency Virus or Bovine Foamy Virus. Retrovirology, 8(Suppl. 1), A22. DOI: 10.1186/1742-4690-8-S1-A22. http://dx.doi.org/10.1186/1742-4690-8-S1-A22CrossrefGoogle Scholar

  • [26] Salazar-Calderón M., Martín-Alonso J.M., Ruiz de Eguino A.D., Casais R., Marin M.S., Parra F. 2000. Fasciola hepatica: Heterologous expression and functional characterization of Thioredoxin Peroxidase. Experimental Parasitology, 95, 63–70. http://dx.doi.org/10.1006/expr.2000.4495CrossrefGoogle Scholar

  • [27] Schweizer G., Braun U., Deplazes P., Torgerson P.R. 2005. Estimating the financial losses due to bovine fasciolosis in Switzerland. Veterinary Records, 157, 188–193. Google Scholar

  • [28] Serradell M.C., Guasconi L., Masih D.T. 2009. Involvement of a mitochondrial pathway and key role of hydrogen peroxide during eosinophil apoptosis induced by excretory-secretory products from Fasciola hepatica. Molecular and Biochemical Parasitology,163, 95–106. DOI: 10.1016/j.molbiopara.2008.10.005. http://dx.doi.org/10.1016/j.molbiopara.2008.10.005CrossrefWeb of ScienceGoogle Scholar

  • [29] Shiels B.R., McKellar S., Katzer F., Lyons K., Kinnaird J., Ward C., Wastling J.M., Swan D. 2004. A Theileria annulata DNA binding protein localized to the host cell nucleus alters the phenotype of a bovine macrophage cell line. Eukaryotic Cell, 3, 495–505. http://dx.doi.org/10.1128/EC.3.2.495-505.2004CrossrefGoogle Scholar

  • [30] Sreekrishna K., Potenz R.H., Cruze J.A., McCombie W.R., Parker K.A,. Nelles L., Mazzaferro P.K., Holden K.A., Harrison R.G., Wood P.J, et al. 1988. High level expression of heterologous proteins in methylotrophic yeast Pichia pastoris. Journal of Basic Microbiology. 28, 265–278. http://dx.doi.org/10.1002/jobm.3620280410CrossrefGoogle Scholar

  • [31] Stabel J.R., Stabel T.J. 1995. Immortalization and characterization of bovine peritoneal macrophages transfected with SV40 plasmid DNA. Veterinary Immunology and Immunopathology, 45, 211–220. http://dx.doi.org/10.1016/0165-2427(94)05348-VCrossrefGoogle Scholar

  • [32] van Riet E., Hartgers F.C., Yazdanbakhsh M. 2007. Chronic helminth infections induce immunomodulation: consequences and mechanisms. Immunobiology, 212, 475–490. DOI: 10.1016/j.imbio.2007.03.009 http://dx.doi.org/10.1016/j.imbio.2007.03.009Web of ScienceCrossrefGoogle Scholar

  • [33] Wasyl K., Zawistowska-Deniziak A., Bąska P., Wędrychowicz H., Wiśniewski M. 2013. Molecular cloning and expression of the cDNA sequence encoding a novel aspartic protease from Uncinaria stenocephala. Experimental Parasitology, 134, 220–227. DOI: 10.1016/j.exppara.2013.03.013. http://dx.doi.org/10.1016/j.exppara.2013.03.013CrossrefWeb of ScienceGoogle Scholar

  • [34] Weaver C.T., Harrington L.E., Mangan P.R., Gavrieli M., Murphy KM. 2006. Th17: an effector CD4 T cell lineage with regulatory T cell ties. Immunity, 24, 677–688. Review. http://dx.doi.org/10.1016/j.immuni.2006.06.002CrossrefGoogle Scholar

  • [35] Wędrychowicz H., Klockiewicz M. 1994. Protective and diagnostic molecules of Fasciola hepatica. Acta Parasitologica, 39, 173–178. Google Scholar

  • [36] Williamson A.L., Brindley P.J., Loukas A. 2003. Hookworm cathepsin D aspartic proteases: contributing roles in the host-specific degradation of serum proteins and skin macromolecules. Parasitology, 126, 179–185. http://dx.doi.org/10.1017/S0031182002002706CrossrefGoogle Scholar

  • [37] Williamson A.L., Lecchi P., Turk B.E., Choe Y., Hotez P.J., McKerrow J.H., Cantley L.C., Sajid M., Craik C.S., Loukas A. 2004. A multi-enzyme cascade of hemoglobin proteolysis in the intestine of blood-feeding hookworms. Journal of Biological Chemistry, 279, 35950–35957. http://dx.doi.org/10.1074/jbc.M405842200CrossrefGoogle Scholar

  • [38] Wilson L.R., Good R.T., Panaccio M., Wijffels G.L., Sandeman R.M., Spithill T.W. 1998. Fasciola hepatica: characterization and cloning of the major cathepsin B protease secreted by newly excysted juvenile liver fluke. Experimental Parasitology, 88,85–94. http://dx.doi.org/10.1006/expr.1998.4234CrossrefGoogle Scholar

  • [39] Zawistowska-Deniziak A., Wasyl K., Norbury L.J., Wesołowska A., Bień J., Grodzik M., Wiśniewski M., Bąska P., Wędrychowicz H. 2013. Characterization and differential expression of cathepsin L3 alleles from Fasciola hepatica. Molecular and Biochemical Parasitology, 190, 27–37. DOI: 10.1016/j.molbiopara.2013.06.001. http://dx.doi.org/10.1016/j.molbiopara.2013.06.001CrossrefGoogle Scholar

About the article

Published Online: 2013-12-13

Published in Print: 2013-12-01


Citation Information: Acta Parasitologica, ISSN (Online) 1896-1851, ISSN (Print) 1230-2821, DOI: https://doi.org/10.2478/s11686-013-0163-5.

Export Citation

© 2013 W. Stefański Institute of Parasitology, PAS. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

[1]
Piotr Bąska, Luke James Norbury, Anna Zawistowska-Deniziak, Marcin Wiśniewski, and Kamil Januszkiewicz
Parasitology Research, 2017
[2]
M Reyes-Becerril, E Alamillo, A Trasviña, I Hirono, H Kondo, W Jirapongpairoj, F Ascencio-Valle, and C Angulo
Journal of Fish Diseases, 2017
[3]
Anna Zawistowska-Deniziak, Katarzyna Basałaj, Barbara Strojny, and Daniel Młocicki
Frontiers in Immunology, 2017, Volume 8
[4]
Luan C. Henker, Claiton I. Schwertz, Neuber J. Lucca, Manoela M. Piva, Keila C. Prior, Piotr Baska, Luke Norbury, Kamil Januszkiewicz, Diogenes Dezen, Marta M.M.F. Duarte, Rafael N. Moresco, Liana Bertagnolli da Rosa, and Ricardo E. Mendes
Vaccine, 2017, Volume 35, Number 4, Page 663
[5]
Ewa Długosz and Marcin Wiśniewski
Acta Parasitologica, 2016, Volume 61, Number 1
[6]
Matheus D. Baldissera, Nathieli B. Bottari, Ricardo E. Mendes, Claiton I. Schwertz, Neuber J. Lucca, Diessica Dalenogare, Guilherme V. Bochi, Rafael N. Moresco, Vera M. Morsch, Maria R.C. Schetinger, Virginia C. Rech, Jeandre A. Jaques, and Aleksandro S. Da Silva
Pathology - Research and Practice, 2015, Volume 211, Number 11, Page 871
[7]
Nathieli B. Bottari, Ricardo E. Mendes, Neuber J. Lucca, Claiton I. Schwertz, Luan C. Henker, Débora C. Olsson, Manoela M. Piva, Manuela Sangoi, Luízi P. Campos, Rafael N. Moresco, Jeandre A. Jaques, and Aleksandro S. Da silva
Experimental Parasitology, 2015, Volume 159, Page 24
[8]
Kamil Januszkiewicz, Luke J. Norbury, Przemysław Wilkowski, Anna Zawistowska-Deniziak, Agnieszka Wesołowska, and Halina Wedrychowicz
Acta Parasitologica, 2015, Volume 60, Number 3

Comments (0)

Please log in or register to comment.
Log in