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Acta Parasitologica

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Volume 59, Issue 1 (Mar 2014)

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PCR detection of Neospora caninum in water buffalo foetal tissues

Clementina Auriemma
  • Department of Animal Health, Istituto Zooprofilattico Sperimentale del Mezzogiorno, Via Salute, 2, 80055, Portici, NA, Italy
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/ Maria Lucibelli
  • Department of Animal Health, Istituto Zooprofilattico Sperimentale del Mezzogiorno, Via Salute, 2, 80055, Portici, NA, Italy
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/ Giorgia Borriello
  • Department of Animal Health, Istituto Zooprofilattico Sperimentale del Mezzogiorno, Via Salute, 2, 80055, Portici, NA, Italy
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/ Esterina Carlo
  • Centro di Referenza Nazionale sull’Igiene e le Tecnologie dell’Allevamento e delle Produzioni Bufaline, S.S. 18 Via delle Calabrie, 27-84131, Fuorni, SA, Italy
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/ Alessandra Martucciello
  • Centro di Referenza Nazionale sull’Igiene e le Tecnologie dell’Allevamento e delle Produzioni Bufaline, S.S. 18 Via delle Calabrie, 27-84131, Fuorni, SA, Italy
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/ Lorena Schiavo
  • Centro di Referenza Nazionale sull’Igiene e le Tecnologie dell’Allevamento e delle Produzioni Bufaline, S.S. 18 Via delle Calabrie, 27-84131, Fuorni, SA, Italy
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/ Amalia Gallo
  • Department of Animal Health, Istituto Zooprofilattico Sperimentale del Mezzogiorno, Via Salute, 2, 80055, Portici, NA, Italy
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/ Francesca Bove
  • Department of Animal Health, Istituto Zooprofilattico Sperimentale del Mezzogiorno, Via Salute, 2, 80055, Portici, NA, Italy
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/ Federica Corrado
  • Department of Animal Health, Istituto Zooprofilattico Sperimentale del Mezzogiorno, Via Salute, 2, 80055, Portici, NA, Italy
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/ Santa Girardi
  • Department of Animal Health, Istituto Zooprofilattico Sperimentale del Mezzogiorno, Via Salute, 2, 80055, Portici, NA, Italy
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/ Maria Amoroso
  • Department of Animal Health, Istituto Zooprofilattico Sperimentale del Mezzogiorno, Via Salute, 2, 80055, Portici, NA, Italy
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/ Barbara Ďegli Uberti
  • Department of Animal Health, Istituto Zooprofilattico Sperimentale del Mezzogiorno, Via Salute, 2, 80055, Portici, NA, Italy
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/ Giorgio Galiero
  • Department of Animal Health, Istituto Zooprofilattico Sperimentale del Mezzogiorno, Via Salute, 2, 80055, Portici, NA, Italy
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Published Online: 2014-02-26 | DOI: https://doi.org/10.2478/s11686-014-0201-y

Abstract

The seroprevalence of Neospora caninum was surveyed by an ELISA kit on two water buffalo herds of Southern Italy. Seropositive samples were detected in 47% and 59% of individuals, respectively, thus indicating high level of exposure to the parasite even if the possibility of vertical transmission cannot be excluded. Tissue samples collected from three aborted fetuses from the same herds were investigated for N. caninum presence by PCR assays targeting the 18S and the Nc5 DNA sequences, respectively. Both methods have shown the presence of N. caninum DNA in heart and brain. Sequencing of the Nc5 genomic DNA confirmed the presence of N. caninum in the samples; phylogenetic analysis of the obtained sequences showed high homology among the Neospora recovered from different samples. The present study suggests an important role of N. caninum as a possible abortive agent for water buffaloes.

Keywords: Neospora caninum; water buffalo; abortion; PCR

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About the article

Published Online: 2014-02-26

Published in Print: 2014-03-01


Citation Information: Acta Parasitologica, ISSN (Online) 1896-1851, DOI: https://doi.org/10.2478/s11686-014-0201-y.

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© 2014 W. Stefański Institute of Parasitology, PAS. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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