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Acta Parasitologica


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Volume 59, Issue 1

Issues

Immunomodulation of ALT-2 and TLR may collude in antigen specific T cell hyporesponsiveness: Proposed mechanism for elevated IL-10 levels in Balb/C mice

Ramanathan Aparnaa
  • Centre for Biotechnology, Anna University, Chennai, 600025, India
  • Centre for Biotechnology, Anna University, Guindy, Chennai, 600025, Tamilnadu, India
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/ Perumal Kaliraj
  • Centre for Biotechnology, Anna University, Chennai, 600025, India
  • Centre for Biotechnology, Anna University, Guindy, Chennai, 600025, Tamilnadu, India
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Published Online: 2014-02-26 | DOI: https://doi.org/10.2478/s11686-014-0204-8

Abstract

ALT-2, a novel antigen belonging to the chromadorea ALT-2 family of the filarial nematode is proved to clear filarial parasites in Jirds. In order to increase the protection efficacy by stimulating the cell mediated immunity, MPLA a detoxified derivative of LPS known to induce the cellular response, was used in this study as an adjuvant on mice models. ALT-2+MPLA formulation elicited a high titer of total IgG antibody, with profoundly increased levels of IgG2b. Reduced splenocyte proliferation was observed in immunized group when compared to control groups which could be attributed to many in vivo factors. The levels of IFN-γ were high in unstimulated MPLA group compared to ALT-2 stimulated MPLA group, suggesting that the ALT-2 antigen suppressed the IFN-γ levels. A high level of IL-10 was induced by the ALT-2+MPLA formulation, which inhibited the production of Th2 cytokines (IL-4, IL-5) and also reduced the Th1 cytokine (IFN-γ, IL-2) levels which are not in vogue with the classical MPLA adjuvant formulation. We propose a mechanism for this immunomodulation which involves a diminished expression of TLR-4, by which the filarial parasites have evolved to evade host immune mechanism.

Keywords: Filariasis; ALT-2; MPLA; TLR; IL-10; immunomodulation

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About the article

Published Online: 2014-02-26

Published in Print: 2014-03-01


Citation Information: Acta Parasitologica, Volume 59, Issue 1, Pages 25–30, ISSN (Online) 1896-1851, DOI: https://doi.org/10.2478/s11686-014-0204-8.

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© 2014 W. Stefański Institute of Parasitology, PAS. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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