Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Acta Parasitologica

IMPACT FACTOR 2017: 1.039
5-year IMPACT FACTOR: 1.121

CiteScore 2018: 1.00

SCImago Journal Rank (SJR) 2018: 0.500
Source Normalized Impact per Paper (SNIP) 2018: 0.664

More options …
Volume 59, Issue 1


Adaptation and immunogenicity of Cryptosporidium parvum to immunocompetent mice

Tomohide Matsuo
  • Laboratory of Parasitology, Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima, 890-0065, Japan
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Yasuko Tsuge / Rika Umemiya-Shirafuji
  • National Research Center for Protozoan Diseases, Obihiro University of Agriculture & Veterinary Medicine, Obihiro, 080-8555, Japan
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Takashi Fujino / Toshihiro Matsui
Published Online: 2014-02-26 | DOI: https://doi.org/10.2478/s11686-014-0218-2


The adaptation and immunogenisity of Cryptosporidium parvum isolated from Siberian chipmunks (SC1 strain) in immunocompetent (ICR) mice were examined. The oocysts were received to the severe combined immunodeficiency (SCID) mice by repeated passage. The oocysts collected from the 18th SCID mice were inoculated to 5 ICR mice. The mice began to shed oocysts from 6 days after inoculation, the patency was 5 days, and the maximum oocysts per gram of feces (OPG) value was 104. The maximum of OPG value was gradually increased by successive passage, and finally that in the 22nd mice reached 106 (patency: 11 days). It is considered that these results indicate completion of their adaptation to ICR mice. To examine the immunogenicity of C. parvum to ICR mice, 8 groups of 5 mice each were inoculated with 1.3 × 106 oocysts of SC1 strain, which were collected after adaptation to SCID mice. All groups shed oocysts from 6th day, and their patency was from 8 to 12 days. On the 21st day after the primary infection, these mice were challenged with 1.3 × 106 oocysts. No oocysts shed from any groups, although 2 control groups shed oocysts from the 6th day, and their OPG values were more than 106. These results suggest that this strain has strong immunogenicity against ICR mice. Therefore, the immunological healthy mice were considered a useful experimental model to investigate immunological and drug treatments in the strain of C. parvum.

Keywords: Cryptosporidium; mice; adaptation; immunogenicity; experimental model

  • [1] Fayer R., Andrew C., Ungar B.L.P., Blagburg B. 1989a. Efficacy of hyperimmune bovine colostrum for prophylaxis of cryptosporidiosis in neonatal calves. Journal of Parasitology, 75, 393–397. DOI: 10.2307/3282595. http://dx.doi.org/10.2307/3282595CrossrefGoogle Scholar

  • [2] Fayer R., Perryman L.E., Riggs M.W. 1989b. Hyperimmune bovine colostrum neutralizes Cryptosporidium sporozoites and protects mice against oocyst challenge. Journal of Parasitology, 75, 151–153. DOI: 10.2307/3282956. http://dx.doi.org/10.2307/3282956CrossrefGoogle Scholar

  • [3] Matsubayashi M., Kimata I., Iseki M., Hajiri T., Tani H., Sasai K., Baba E. 2005. Infectivity of a novel type of Cryptosporidium andersoni to laboratory mice. Veterinary Parasitology, 129, 165–168. DOI: 10.1016/j.vetpar2005.01.003. http://dx.doi.org/10.1016/j.vetpar.2005.01.003CrossrefGoogle Scholar

  • [4] Matsubayashi M., Kimata I., Abe N., Tani H., Sasai K. 2004. The detection of a novel type of Cryptosporidium andersoni oocyst in cattle in Japan. Parasitology Research, 93, 504–506. DOI: 10.1007/s00436-004-1153-1. http://dx.doi.org/10.1007/s00436-004-1153-1CrossrefPubMedGoogle Scholar

  • [5] Matsui T., Fujino T., Kajima J., Tsuji M. 2000. Infectivity to experimental rodents of Cryptosporidium parvum oocysts from Siberian Chipmunks (Tamias sibiricus) originated in the People’s Republic of China. Journal of Veterinary Medical Science, 62, 487–489. DOI: 10.1292/jvms.62.487. http://dx.doi.org/10.1292/jvms.62.487CrossrefGoogle Scholar

  • [6] Matsui T., Fujino T., Kobayashi F., Morii T., Tsuji M. 1994. Oocyst production and immunogenisity of Cryptosporidium muris in the experimental mice. Japanese Journal of Parasitology, 43, 199–204. (in Japanese) Google Scholar

  • [7] Matsui T., Morii T., Fujino T., Tadeja S.L., Itakura C. 1992. Oocyst production and immunogenisity of Cryptosporidium sp. in chickens. Journal of Parasitology, 41, 24–29. Google Scholar

  • [8] McDonald V., Deer R., Uni S., Iseki M., Bancroft G.J. 1992. Immune responses to Cryptosporidium muris and Cryptosporidium parvum in adult immunocompetent or immunocompromised (nude or SCID) mice. Infection and Immunity, 60, 3325–3331. PubMedGoogle Scholar

  • [9] Tzipori S., Roberton D., Chapman C. 1986. Remission of diarrhea due to cryptosporidiosis in an immunodeficient child treated with hyperimmune bovine colostrum. British Medical Journal, 293, 1276–1277. http://dx.doi.org/10.1136/bmj.293.6557.1276Google Scholar

  • [10] Umemiya R., Fukuda M., Fujisaki K., Matsui T. 2005. Electron microscopic observation of the invasion process of Cryptosporidium parvum in severe combined immunodeficiency mice. Journal of Parasitology, 91, 1034–1039. DOI: 10.1645/GE-508R.1. http://dx.doi.org/10.1645/GE-508R.1CrossrefGoogle Scholar

About the article

Published Online: 2014-02-26

Published in Print: 2014-03-01

Citation Information: Acta Parasitologica, Volume 59, Issue 1, Pages 189–192, ISSN (Online) 1896-1851, DOI: https://doi.org/10.2478/s11686-014-0218-2.

Export Citation

© 2014 W. Stefański Institute of Parasitology, PAS. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

N. A. Filatova, N. A. Knyazev, S. O. Skarlato, O. V. Anatskaya, and A. E. Vinogradov
Parasite Immunology, 2018, Page e12524

Comments (0)

Please log in or register to comment.
Log in