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BY-NC-ND 3.0 license Open Access Published by De Gruyter February 26, 2014

Molecular analysis of lungworms from European bison (Bison bonasus) on the basis of small subunit ribosomal RNA gene (SSU)

  • Anna Pyziel EMAIL logo
From the journal Acta Parasitologica

Abstract

Dictyocaulosis (Nematoda: Trichostrongyloidea) is a widespread parasitosis of the European bison (Bison bonasus) inhabiting Bialowieza Primeval Forest. Bearing in mind the current coexistence of bison with wild cervids, and with domestic ruminants in the 19th and 20th century, the need arose for molecular identification of lungworm species. Molecular analysis was done on adult lungworms that were obtained from the respiratory track of four free-roaming bison euthanized as a part of the population health control program. As the result of the study four identical small subunit-ribosomal RNA gene sequences from the lungworms were obtained and deposited in GenBank as sequence, 1708 bp long (GenBank KC771250). Comparative analysis of the SSU rRNA sequences revealed the European bison to be a host for the bovine lungworm Dictyocaulus viviparus.

[1] Chilton N.B., Huby-Chilton F., Gasser R.B., Beveridge I. 2006. The evolutionary origins of nematodes within the order Strongylida are related to predilection sites within hosts. Molecular Phylogenetics and Evolution, 40, 118–128. DOI: 10.1016/j.ympev.2006.01.003. http://dx.doi.org/10.1016/j.ympev.2006.01.00310.1016/j.ympev.2006.01.003Search in Google Scholar PubMed

[2] Demiaszkiewicz A.W., Pyziel A.M., Lachowicz J. 2008. Stan zarazenia zubrow w Puszczy Bialowieskiej helmintami w sezonie zimowym 2007/2008. European Bison Conservation Newsletter, 1, 42–52. Search in Google Scholar

[3] Demiaszkiewicz A.W., Pyziel A.M., Lachowicz J., Kuligowska I. 2009. Robaczyca plucna zubrow w Puszczy Bialowieskiej. European Bison Conservation Newsletter, 2, 112–118. Search in Google Scholar

[4] Divina B.P., Wilhelmsson E., Mattsson J.G., Waller P., Hoglund J. 2000. Identification of Dictyocaulus spp. in ruminants by morphological and molecular analyses. Parasitology, 121, 193–201. http://dx.doi.org/10.1017/S003118209900616210.1017/S0031182099006162Search in Google Scholar

[5] Gasser R.B., Jabbar A., Mohandas N., Hoglund J., Hall R.S., Littlewood D.T.J., Jex A.R. 2012. Assessment of the genetic relationship between Dictyocaulus species from Bos taurus and Cervus elaphus using complete mitochondrial genomic datasets. Parasites & Vectors, 5, 241. DOI: 10.1186/1756-3305-5-241. http://dx.doi.org/10.1186/1756-3305-5-24110.1186/1756-3305-5-241Search in Google Scholar PubMed PubMed Central

[6] Hoglund J., Morrison D.A., Divina B.P., Wilhelmsson E. 2003. Phylogeny of Dictyocaulus (lungworms) from eight species of ruminants based on analyses of ribosomal RNA data. Parasitology, 127, 179–187. DOI: 10.1017/S0031182003366. http://dx.doi.org/10.1017/S0031182003003366Search in Google Scholar

[7] Hoglund J., Morrison D.A., Mattsson J.G., Engstrom A. 2006. Population genetics of the bovine/cattle lungworm (Dictyocaulus viviparus) based on mtDNA and AFLP marker techniques. Parasitology, 133, 89–99. DOI: 10.1017/S0031182006009991. http://dx.doi.org/10.1017/S003118200600999110.1017/S0031182006009991Search in Google Scholar PubMed

[8] Koffman M. 1942. Bidrag till kannedomen om parasiter hos husdjur och vilt i Sverige. III. Parasitofauna hos bisonoxe, visent (Bison Europeus). Meddelanden fran Statens veterinarbakteriologiska anstalt, 168. Search in Google Scholar

[9] Nicholas K.B., Nicholas H.B. Jr., Deerfield D.W. 1997. GeneDoc: Analysis and visualization of genetic variation. EMBNEW NEWS, 4, 14. Search in Google Scholar

[10] Wroblewski K. (Ed.). 1927. Zubr Puszczy Bialowieskiej. Wydawnictwo Polskie, Poznan, Poland. Search in Google Scholar

Published Online: 2014-2-26
Published in Print: 2014-3-1

© 2014 W. Stefański Institute of Parasitology, PAS

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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