Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Acta Parasitologica


IMPACT FACTOR 2017: 1.039
5-year IMPACT FACTOR: 1.121

CiteScore 2018: 1.00

SCImago Journal Rank (SJR) 2018: 0.500
Source Normalized Impact per Paper (SNIP) 2018: 0.664

More options …
Volume 59, Issue 1

Issues

High occurrence of Cryptosporidium ubiquitum and Giardia duodenalis genotype E in sheep from Brazil

Flávio Paz e Silva
  • Laboratory of Parasitic Diseases, Department of Veterinary Clinic, Faculty of Veterinary Medicine and Animal Science, UNESP — Univ Estadual Paulista, Botucatu, São Paulo, Brazil
  • Laboratory of Molecular Diagnosis, Department of Microbiology and Immunology, Biosciences Institute, UNESP — Univ Estadual Paulista, Botucatu, São Paulo, Brazil
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Raimundo Lopes
  • Laboratory of Parasitic Diseases, Department of Veterinary Clinic, Faculty of Veterinary Medicine and Animal Science, UNESP — Univ Estadual Paulista, Botucatu, São Paulo, Brazil
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Katia Bresciani
  • Departamento de Apoio, Produção e Saúde Animal, FMVA, UNESP — Univ Estadual Paulista, Araçatuba, São Paulo, Brazil
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Alessandro Amarante / João Araujo
  • Laboratory of Molecular Diagnosis, Department of Microbiology and Immunology, Biosciences Institute, UNESP — Univ Estadual Paulista, Botucatu, São Paulo, Brazil
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2014-02-26 | DOI: https://doi.org/10.2478/s11686-014-0223-5

Abstract

In this paper we report the occurrence of zoonotic species of Cryptosporidium (C. ubiquitum and C. parvum subtype IIaA15G2R1) and the host-specific Giardia duodenalis genotype E in a sheep farm from Brazil. Fecal specimens were collected from 100 animals and screened for the presence of G. duodenalis and Cryptosporidium spp. by enzyme immunoassay (EIA) and polymerase chain reaction (PCR). Restriction fragment length polymorphism (RFLP) and sequence analysis of selected samples was used to confirm the species and to characterize the isolates. Of 100 specimens, 34 were positive for Giardia (34%) and 25 were positive for Cryptosporidium (25%). The prevalence of Giardia and Cryptosporidium was higher in lambs than in ewes. Co-infection was observed in 14% of animals. All G. duodenalis isolates belonged to genotype E. The genetic characterization performed in 22 Cryptosporidium-positive samples revealed the presence of zoonotic C. ubiquitum and C. parvum in 76% (19/25) and 12% (3/25) of infected animals, respectively. The presence of zoonotic Cryptosporidium subtypes in lambs was of epidemiological and public health relevance.

Keywords: Cryptosporidium; Giardia; genotypes; sheep; zoonosis

  • [1] Aloisio F., Filippini G., Antenucci P., Lepri E., Pezzotti G., Cacciò S.M., Pozio E. 2006. Severe weight loss in lambs infected with Giardia duodenalis assemblage B. Veterinary Parasitology, 142, 154–158. DOI: 10.1016/j.vetpar.2006.06.023. http://dx.doi.org/10.1016/j.vetpar.2006.06.023CrossrefGoogle Scholar

  • [2] Amer S., Harfoush M., He H. 2010. Cryptosporidium genotypes and subtypes in dairy calves in Egypt. Veterinary Parasitology, 169, 382–386. DOI: 10.1016/j.vetpar.2010.01.017. http://dx.doi.org/10.1016/j.vetpar.2010.01.017Web of ScienceCrossrefGoogle Scholar

  • [3] Elwin K., Chalmers R.M. 2008. Contemporary identification of previously reported novel Cryptosporidium isolates reveals Cryptosporidium bovis and the cervine genotype in sheep (Ovis aries). Parasitology Research, 102, 1103–1105. DOI: 10.1007/s00436-008-0935-2. http://dx.doi.org/10.1007/s00436-008-0935-2Web of ScienceCrossrefGoogle Scholar

  • [4] Fayer R., Santín M. 2009. Cryptosporidium xiaoi n. sp. (Apicomplexa: Cryptosporidiidae) in sheep (Ovis aries). Veterinary Parasitology, 164, 192–200. DOI: 10.1016/j.vetpar.2009.05.011. http://dx.doi.org/10.1016/j.vetpar.2009.05.011Web of ScienceCrossrefGoogle Scholar

  • [5] Fayer R., Santín M., Macarisin D. 2010. Cryptosporidium ubiquitum n. sp. in animals and humans. Veterinary Parasitology, 172, 23–32. DOI: 10.1016/j.vetpar.2010.04.028. http://dx.doi.org/10.1016/j.vetpar.2010.04.028Web of ScienceCrossrefGoogle Scholar

  • [6] Feng Y., Ortega Y., He G., Das P., Xu M., Zhang X., Fayer R., Gatei W., Cama V., Xiao L. 2007. Wide geographic distribution of Cryptosporidium bovis and the deer-like genotype in bovines. Veterinary Parasitology, 144:1–9. DOI: 10.1016/j.vetpar.2006.10.001 http://dx.doi.org/10.1016/j.vetpar.2006.10.001CrossrefWeb of ScienceGoogle Scholar

  • [7] Fiuza V.R., Cosendey R.I., Frazão-Teixeira E., Santín M., Fayer R., de Oliveira F.C. 2011. Molecular characterization of Cryptosporidium in Brazilian sheep. Veterinary Parasitology, 175, 360–362. DOI: 10.1016/j.vetpar.2010.10.036. http://dx.doi.org/10.1016/j.vetpar.2010.10.036CrossrefGoogle Scholar

  • [8] Geurden T., Thomas P., Casaert S., Vercruysse J., Claerebout E. 2008. Prevalence and molecular characterisation of Cryptosporidium and Giardia in lambs and goat kids in Belgium. Veterinary Parasitology, 155, 142–145. DOI: 10.1016/j.vetpar.2008.05.002. http://dx.doi.org/10.1016/j.vetpar.2008.05.002CrossrefGoogle Scholar

  • [9] Giangaspero A., Paoletti B, Lorio R, Traversa D. 2005. Prevalence and molecular characterization of Giardia duodenalis from sheep in central Italy. Parasitology Research, 96:32–37. DOI: 10.1007/s00436-005-1317-7. http://dx.doi.org/10.1007/s00436-005-1317-7Web of ScienceCrossrefGoogle Scholar

  • [10] Gómez-Muñoz M.T., Navarro C., Garijo-Toledo M.M., Dea-Ayuela M.A., Fernández-Barredo S., Pérez-Gracia M.T., Domínguez-Márquez M.V., Borrás R. 2009. Occurrence and genotypes of Giardia isolated from lambs in Spain. Parasitology International, 58, 297–299. DOI: 10.1016/j.parint.2009.06.005. http://dx.doi.org/10.1016/j.parint.2009.06.005CrossrefWeb of ScienceGoogle Scholar

  • [11] Green R.E., Amarante A.F.T., Mascarini L.M. 2004. The seasonal distribuition of Cryptosporidium oocysts in sheep raised in the state of Sao Paulo. Revista Brasileira de Parasitologia Veterinária, 13, 125–127. DOI: 10.1590/S1984-29612010000400002. CrossrefGoogle Scholar

  • [12] Meireles M.V. 2010. Cryptosporidium infection in Brazil: implications for veterinary medicine and public health. Revista Brasileira de Parasitologia Veterinária, 19, 197–204. DOI: 10.1590/S1984-29612010000400002. http://dx.doi.org/10.1590/S1984-29612010000400002CrossrefGoogle Scholar

  • [13] Pangasa A., Jex A.R., Nolan M.J., Campbell B.E., Haydon S.R., Stevens M.A., Gasser R.B. 2010. Highly sensitive nonisotopic restriction endonuclease fingerprinting of nucleotide variability in the gp60 gene within Cryptosporidium species, genotypes and subgenotypes infective to humans, and its implications Electrophoresis, 31, 1637–1647. DOI: 10.1002/elps.200900706. http://dx.doi.org/10.1002/elps.200900706CrossrefWeb of ScienceGoogle Scholar

  • [14] Peng M.M., Wilson M.L., Holland R.E., Meshnick S.R., Lal A.A., Xiao L. 2003. Genetic diversity of Cryptosporidium spp. in cattle in Michigan: implications for understanding the transmission dynamics. Parasitology Research, 90, 175–180. DOI: 10.1007/s00436-003-0834-5. http://dx.doi.org/10.1007/s00436-003-0843-4CrossrefGoogle Scholar

  • [15] Read C.M., Monis P.T., Thompson R.C.A. 2004. Discrimination of all genotypes of Giardia duodenalis at the glutamate dehydrogenase locus using PCR-RFLP. Infection Genetics and Evolution, 4, 125–130. DOI: 10.1016/j.meegid.2004.02.001. http://dx.doi.org/10.1016/j.meegid.2004.02.001CrossrefGoogle Scholar

  • [16] Robertson L.J., Gjerde B.K., Furuseth H.E. 2010. The zoonotic potential of Giardia and Cryptosporidium in Norwegian sheep: a longitudinal investigation of 6 flocks of lambs. Veterinary Parasitology, 171, 140–145. DOI: 0.1016/j.vetpar.2010.03.014. http://dx.doi.org/10.1016/j.vetpar.2010.03.014Web of ScienceGoogle Scholar

  • [17] Rocha R.A., Bresciani K.D.S., Barros T.F.M., Fernandes L.H., Silva M.B., Amarante A.F.T. 2008. Sheep and cattle grazing alternately: Nematode parasitism and pasture decontamination. Small Ruminant Research, 75, 135–143. DOI: 10.1016/j.smallrumres.2007.09.001 http://dx.doi.org/10.1016/j.smallrumres.2007.09.001Web of ScienceCrossrefGoogle Scholar

  • [18] Ryan U.M., Bath C., Robertson I., Read C., Elliot A., Mcinnes L., Traub R., Besier B. 2005. Sheep may not be an important zoonotic reservoir for Cryptosporidium and Giardia parasites. Applied Environmental Microbiology, 71, 4992–4497. DOI: 10.1128/AEM.71.9.4992-4997.2005. http://dx.doi.org/10.1128/AEM.71.9.4992-4997.2005CrossrefGoogle Scholar

  • [19] Santín M., Trout J.M., Fayer R. 2007. Prevalence and molecular characterization of Cryptosporidium and Giardia species and genotypes in sheep in Maryland. Veterinary Parasitology, 146, 17–24. DOI: 10.1016/j.vetpar.2007.01.010. http://dx.doi.org/10.1016/j.vetpar.2007.01.010CrossrefGoogle Scholar

  • [20] Trotz-Williams L.A., Martin D., Gatei W., Cama V., Peregrine A.S., Martin S.W., Nydam D.V., Jamieson F., Xiao L. 2006. Genotype and subtype analyses of Cryptosporidium isolates from dairy calves and humans in Ontario. Parasitology Research, 99, 346–352. DOI: 10.1007/s00436-006-0157-4. http://dx.doi.org/10.1007/s00436-006-0157-4CrossrefGoogle Scholar

  • [21] Xiao L., Singh A., Limor J., Graczyk T.K., Gradus S., Lal A. 2001. Molecular characterization of Cryptosporidium oocysts in samples of raw surface water and wastewater. Applied Environmental Microbiology, 67, 1097–1101. DOI: 10.1128/AEM.67.3.1097-1101.2001 http://dx.doi.org/10.1128/AEM.67.3.1097-1101.2001CrossrefGoogle Scholar

  • [22] Wang Y., Feng Y., Cui B., Jian F., Ning C., Wang R., Zhang L., Xiao L. 2010. Cervine genotype is the major Cryptosporidium genotype in sheep in China. Parasitology Research, 106, 341–347. DOI: 10.1007/s00436-009-1664-x. http://dx.doi.org/10.1007/s00436-009-1664-xWeb of ScienceCrossrefGoogle Scholar

  • [23] Yang R., Jacobson C., Gordon C., Ryan U. 2009. Prevalence and molecular characterisation of Cryptosporidium and Giardia species in pre-weaned sheep in Australia. Veterinary Parasitology, 161, 19–24. DOI: 10.1016/j.vetpar.2008.12.021. http://dx.doi.org/10.1016/j.vetpar.2008.12.021CrossrefGoogle Scholar

About the article

Published Online: 2014-02-26

Published in Print: 2014-03-01


Citation Information: Acta Parasitologica, Volume 59, Issue 1, Pages 193–196, ISSN (Online) 1896-1851, DOI: https://doi.org/10.2478/s11686-014-0223-5.

Export Citation

© 2014 W. Stefański Institute of Parasitology, PAS. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

[1]
Sha-Sha Wang, Ya-Jie Yuan, Yan-Ling Yin, Rui-Si Hu, Jun-Ke Song, and Guang-Hui Zhao
Parasites & Vectors, 2017, Volume 10, Number 1
[2]
Qingxun Zhang, Zhichao Zhang, Sitong Ai, Xiaoqi Wang, Ruiying Zhang, and Ziyuan Duan
Comparative Immunology, Microbiology and Infectious Diseases, 2019, Page 101346
[3]
Magdy Elsayed MAHFOUZ, Nabila MIRA, and Said AMER
Journal of Veterinary Medical Science, 2014, Volume 76, Number 12, Page 1569
[4]
Olufemi Ambrose Akinkuotu, Michael Irewole Takeet, Ebenezer Babatunde Otesile, Frederick Olufemi, Spencer J. Greenwood, and J. Trenton McClure
Acta Tropica, 2019, Volume 195, Page 15
[5]
Lynda Sahraoui, Myriam Thomas, Aurélie Chevillot, Mohamed Mammeri, Bruno Polack, Isabelle Vallée, Jérôme Follet, Hacina Ain-Baaziz, and Karim Tarik Adjou
Veterinary Parasitology: Regional Studies and Reports, 2019, Volume 16, Page 100280
[6]
Rongsheng Mi, Xiaojuan Wang, Yan Huang, Guodong Mu, Yehua Zhang, Haiyan Jia, Xiaoli Zhang, Heng Yang, Xu Wang, Xiangan Han, Zhaoguo Chen, and Charles M. Dozois
Applied and Environmental Microbiology, 2018, Volume 84, Number 18
[7]
Luciane Holsback, Heloísa Eid Lima, Odilon Vidotto, Marcelo Alves da Silva, Thaís Helena Constantino Patelli, Felippe Danyel Cardoso Martins, and Mércia de Seixas
Revista Brasileira de Parasitologia Veterinária, 2018
[8]
P. Díaz, E. Navarro, A. Prieto, A. Pérez-Creo, M. Viña, J.M. Díaz-Cao, C.M. López, R. Panadero, G. Fernández, P. Díez-Baños, and P. Morrondo
Veterinary Parasitology, 2018
[9]
Yingna Jian, Xueyong Zhang, Xiuping Li, Gabriele Karanis, Liqing Ma, and Panagiotis Karanis
Veterinary Parasitology, 2018, Volume 250, Page 40
[10]
Camila Henriques Coelho, Maurício Durigan, Diego Averaldo Guiguet Leal, Adriano de Bernardi Schneider, Regina Maura Bueno Franco, Steven M. Singer, and Ricardo Toshio Fujiwara
PLOS Neglected Tropical Diseases, 2017, Volume 11, Number 10, Page e0006005
[11]
Teklu Wegayehu, Md Robiul Karim, Junqiang Li, Haileeyesus Adamu, Berhanu Erko, Longxian Zhang, and Getachew Tilahun
BMC Veterinary Research, 2016, Volume 13, Number 1
[12]
Agnieszka Kaupke, Mirosław M. Michalski, and Artur Rzeżutka
Parasitology Research, 2017, Volume 116, Number 3, Page 871
[13]
D.C. Chatzopoulos, S. Sarrou, N.G.C. Vasileiou, K.S. Ioannidi, E. Peteinaki, G. Valiakos, C.N. Tsokana, E. Papadopoulos, V. Spyrou, V.S. Mavrogianni, A. Giannakopoulos, A. Sbiraki, D. Lacasta, J.P. Bueso, L.V. Athanasiou, C. Billinis, and G.C. Fthenakis
Small Ruminant Research, 2016, Volume 141, Page 5
[14]
Qiao Li, Lu Li, Wei Tao, Yanxue Jiang, Qiang Wan, Yongchao Lin, and Wei Li
Parasitology Research, 2016, Volume 115, Number 7, Page 2905
[15]
Satomi Kato, Yojiro Yanagawa, Ryota Matsuyama, Masatsugu Suzuki, and Chihiro Sugimoto
Parasitology Research, 2016, Volume 115, Number 4, Page 1463
[16]
Pablo Díaz, Joaquín Quílez, Alberto Prieto, Esther Navarro, Ana Pérez-Creo, Gonzalo Fernández, Rosario Panadero, Ceferino López, Pablo Díez-Baños, and Patrocinio Morrondo
Parasitology Research, 2015, Volume 114, Number 11, Page 4099
[17]
Jianbin Ye, Lihua Xiao, Yuanfei Wang, Yaqiong Guo, Dawn M. Roellig, and Yaoyu Feng
Veterinary Parasitology, 2015, Volume 210, Number 3-4, Page 235
[18]
M. Koinari, A.J. Lymbery, and U.M. Ryan
Experimental Parasitology, 2014, Volume 141, Page 134

Comments (0)

Please log in or register to comment.
Log in