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Acta Parasitologica

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Volume 59, Issue 2


The effect of initial dose on the recovery and final yields of Heterorhabditis megidis (Rhabditida: Heterorhabditidae) in larvae of the great wax moth, Galleria mellonella

Dorota Tumialis
  • Faculty of Animal Sciences, Department of Animal Environment Biology, Warsaw University of Life Sciences — SGGW, Ciszewskiego 8, 02-786, Warsaw, Poland
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/ Elżbieta Pezowicz
  • Faculty of Animal Sciences, Department of Animal Environment Biology, Warsaw University of Life Sciences — SGGW, Ciszewskiego 8, 02-786, Warsaw, Poland
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/ Anna Mazurkiewicz
  • Faculty of Animal Sciences, Department of Animal Environment Biology, Warsaw University of Life Sciences — SGGW, Ciszewskiego 8, 02-786, Warsaw, Poland
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/ Iwona Skrzecz
  • Department of Forest Protection, Sękocin Stary, Forest Research Institute, Braci Leśnej 3, 05-090, Raszyn, Poland
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/ Elżbieta Popowska-Nowak
  • Faculty of Biology and Environmental Sciences, Cardinal Stefan Wyszynski University, Wóycickiego 1/3, 01-938, Warsaw, Polan
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/ Agnieszka Petrykowska
  • Faculty of Animal Sciences, Department of Animal Environment Biology, Warsaw University of Life Sciences — SGGW, Ciszewskiego 8, 02-786, Warsaw, Poland
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Published Online: 2014-05-15 | DOI: https://doi.org/10.2478/s11686-014-0231-5


The aim of this study was to determine the effect of different initial doses of the infective juveniles (IJs) (50 IJs, 200 IJs, 1000 IJs) of Heterorhabditis megidis Poinar (Rhabditida: Heterorhabditidae) strain IsM15/09 on recovery, final yields and percent final yields in larvae Galleria mellonella ( L.). Percent recovery was not directly related to initial dose. Final yields also did not change with the initial dose. However, percent yields was highly negatively correlated with initial dose of nematodes and was the highest with the 50 IJs dose. Additional point of the study was to investigate whether the nematodes are able to produce progeny from one hermaphroditic individual. The results showed that the invasive larvae resumed growth and transformed into hermaphroditic individuals that reproduced without cross-fertilisation.

Keywords: Heterorhabditis megidis; Galleria mellonella; initial dose; recovery; final yields

  • [1] Baliadi Y., Kondo E., Yoshiga T. 2009. The continual forming and contribution of infective juveniles produced via endotokia matricida of entomopathogenic nematodes in the family of Steinernematidae and Heterorhabditidae. Indonesian Journal of Agricultural Science, 10, 26–33. Google Scholar

  • [2] Boff M.I.C., Wiegers G.L., Gerritsen L.J.M., Smits P.H. 2000. Development of the entomopathogenic nematode Heterorhabditis megidis strain NLH-E 87.3 in Galleria mellonella. Nematology, 2, 303–308. DOI: 10.1163/156854100509178. http://dx.doi.org/10.1163/156854100509178CrossrefGoogle Scholar

  • [3] Boff M.I.C., Wiegers G.L., Smits P.H. 2001. Host influences on the pathogenicity of Heterorhabditis megidis. BioControl, 46, 91–103. DOI: 10.1590/S1519-69842013000200003. http://dx.doi.org/10.1023/A:1009937407899CrossrefGoogle Scholar

  • [4] Bonifassi E., Fischer-Le Saux M., Boemare N., Lanois A., Laumond C., Smart G. 1999. Gnotobiological study of infective juveniles and symbionts of Steinernema scapterisci: A model to clarify the concept of the natural occurrence of monoxenic associations in entomopathogenic nematodes. Journal of Invertebrate Pathology, 74, 164–172. DOI: 10.1098/rspb.2001.1795. http://dx.doi.org/10.1006/jipa.1999.4866CrossrefGoogle Scholar

  • [5] Burnell A.M., Stock S.P. 2000. Heterorhabditis, Steinernema and their bacterial symbionts lethal pathogens of insects. Nematology, 2, 31–42. DOI: 10.1603/029.102.0348. http://dx.doi.org/10.1163/156854100508872CrossrefGoogle Scholar

  • [6] Ehlers R.U., Niemann I., Hollmer S., Strauch O., Jende D., Shanmugasundaram M., Mehta U.K., Easwaramoorthy S.K., Burnell A.M. 2000. Mass production potential of the bactohelminthic biocontrol complex Heterorhabditis indica — Photorhabdus luminescens. Biocontrol Science and Technology, 10, 607–616. DOI: 10.1007/s10123-003-0144-x. http://dx.doi.org/10.1080/095831500750016406CrossrefGoogle Scholar

  • [7] Elawad S.A., Gowen S.R., Hague N.G.M. 1999. The life cycle of Steinernema abbasi and S. riobrave in Galleria mellonella. Nematology, 1, 762–764. http://dx.doi.org/10.1163/156854199508676Google Scholar

  • [8] Fan X., Hominick W.M. 1991. Efficiency of the Galleria (wax moth) baiting technique for recovering infective stages of entomopathogenic rhabditidis (Steinernematidae and Heterorhabditidae) from sand and soil. Revue de Nématologie, 14, 381–387. Google Scholar

  • [9] Flanders K.L., Miller J.M., Shields E.J. 1996. In vivo production of Heterorhabditis bacteriophora ‘Oswego’ (Rhabditida: Heterorhabditidae), a potential biological control agent for soilinhabiting insects in temperate regions. Journal of Economic Entomology, 89, 373–380. CrossrefGoogle Scholar

  • [10] Gerritsen L.J.M., Wiegers G.L., Smits P.H. 1998. Pathogenicity of New Combinations of Heterorhabditis spp. and Photorhabdus luminescens against Galleria mellonella and Tipula olerace. Biological Control, 13, 9–15. http://dx.doi.org/10.1006/bcon.1998.0640Google Scholar

  • [11] Han R.C. 1996. The effects of inoculum size on yield of Steinernema carpocapsae and Heterorhabditis bacteriophora in liquid culture. Nematologica, 42, 546–553. DOI: 10.1163/004625996X 00045. http://dx.doi.org/10.1163/004625996X00045CrossrefGoogle Scholar

  • [12] Kaya H.K., Stock S.P. 1997. Techniques in insect nematology. In: (Ed. L.A. Lacey) Manual of techniques in insect pathology. Academic Press, London, 281–324. http://dx.doi.org/10.1016/B978-012432555-5/50016-6Google Scholar

  • [13] Koppenhöfer A.M., Grewal P.S., Fuzy E.M. 2007. Differences in penetration routes and establishment rates of four entomopathogenic nematode species into four white grub species. Journal of Invertebrate Pathology, 94, 184–195. DOI: 10.1016/j.jip.2006.10.005. http://dx.doi.org/10.1016/j.jip.2006.10.005Web of ScienceCrossrefGoogle Scholar

  • [14] Lacey L.A., Frutos R., Kaya H.K., Vail P. 2001. Insect Pathogens as Biological Control Agents: Do they Have a Future?. Biological Control, 21, 230–248. DOI: 10.1006/bcon.2001.0938. http://dx.doi.org/10.1006/bcon.2001.0938CrossrefGoogle Scholar

  • [15] Li X.Y., Cowles R.S., Cowles E.A., Gaugler R., Cox-Foster D.L. 2007. Relationship between the successful infection by entomopathogenic nematodes and the host immune response. International Journal of Parasitology, 37, 365–374. DOI: 10.1016/j.ijpara.2006.08.009. http://dx.doi.org/10.1016/j.ijpara.2006.08.009CrossrefWeb of ScienceGoogle Scholar

  • [16] Lewis E.E., Campbell J., Griffin C., Kaya H., Peters A. 2006. Behavioral ecology of entomopathogenic nematodes. Biological Control, 38, 66–79. DOI: 10.1016/j.biocontrol.2005.11.007. http://dx.doi.org/10.1016/j.biocontrol.2005.11.007CrossrefGoogle Scholar

  • [17] Mannion C.M., Jansson R.K. 1993. Infectivity of five entomopathogenic nematodes to the sweetpotato weevil, Cylas formicarius (F.), (Coleoptera: Apionidae) in three experimental arenas. Journal of Invertebrate Pathology, 62, 29–36. DOI: 10.1006/jipa.1993.1070. http://dx.doi.org/10.1006/jipa.1993.1070CrossrefGoogle Scholar

  • [18] Mason J.M., Hominick W.M. 1995. The effect of temperature on infection, development and reproduction of Heterorhabditis. Journal of Helminthology, 69, 37–347. DOI: 10.1017/S00 22149X00014929. http://dx.doi.org/10.1017/S0022149X00014929CrossrefGoogle Scholar

  • [19] Saunders J.E., Webster J.M. 1999. Temperature Effects on Heterorhabditis megidis and Steinernema carpocapsae. Infectivity to Galleria mellonella. Journal of Nematology, 31, 299–304. Google Scholar

  • [20] Selvan S., Campbell J.F., Gaugler R. 1993. Density — depndent effects on entomopathogenic nematodes (Heterorhabditidae and Steinernematidae) within an insect host. Journal of Invertebrate Pathology, 62, 278–274. DOI: 10.1006/jipa.1993.1113. http://dx.doi.org/10.1006/jipa.1993.1113CrossrefGoogle Scholar

  • [21] Shapiro-Ilan D.I., Gaugler R. 2002. Production technology for entomopathogenic nematodes and their bacterial symbionts. Journal of Industrial Microbiology and Biotechnology, 28, 137–146. DOI: 10.1038/sj/jim/7000230. http://dx.doi.org/10.1038/sj.jim.7000230CrossrefGoogle Scholar

  • [22] Simões N., Caldas C., Rosa J.S., Bonifassi E., Laumond C. 2000. Pathogenicity Caused by High Virulent and Low Virulent Strains of Steinernema carpocapsae to Galleria mellonella. Journal of Invertebrate Pathology, 75, 47–54. DOI: 10. 1006/jipa.1999.4899. http://dx.doi.org/10.1006/jipa.1999.4899CrossrefGoogle Scholar

  • [23] Wang J., Bedding R.A. 1996. Population development of Heterorhabditis bacteriophora and Steinernema carpocapsae in the larvae of Galleria mellonella. Fundamental and Applied Nematology, 19, 363–367. Google Scholar

  • [24] White G.F. 1929. A method for obtaining infective nematode larvae from cultures. Science, 66, 302–303. DOI: 10.1126/science. 66.1709.302-a. http://dx.doi.org/10.1126/science.66.1709.302-aCrossrefGoogle Scholar

  • [25] Zervos S., Johnson S.C., Webster J.M. 1991. Effect of temperature and inoculum size on reproduction and development of Heterorhabditis heliothidis and Steinernema glaseri (Nematoda: Rhabditoidea) in Galleria mellonella. Canadian Journal of Zoology, 69, 1261–1264. DOI: 10.1139/z91-177. http://dx.doi.org/10.1139/z91-177CrossrefGoogle Scholar

About the article

Published Online: 2014-05-15

Published in Print: 2014-06-01

Citation Information: Acta Parasitologica, Volume 59, Issue 2, Pages 213–218, ISSN (Online) 1896-1851, DOI: https://doi.org/10.2478/s11686-014-0231-5.

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© 2014 W. Stefański Institute of Parasitology, PAS. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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