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Acta Parasitologica

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Volume 59, Issue 2


Seroprevalence of Neospora caninum infection in dairy cows in Northern provinces, Thailand

Tawin Inpankaew
  • National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido, 080-8555, Japan
  • Department of Parasitology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, 10903, Thailand
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/ Sathaporn Jittapalapong / Thomas Mitchell
  • Animal Health Laboratories, Department of Agriculture and Food Western Australia, 3 Baron-Hay Court, South Perth, Western Australia, 6151, Australia
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/ Chainirun Sununta / Ikuo Igarashi
  • National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido, 080-8555, Japan
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/ Xuenan Xuan
  • National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido, 080-8555, Japan
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Published Online: 2014-05-15 | DOI: https://doi.org/10.2478/s11686-014-0245-z


Neospora caninum, an obligate intracellular protozoan parasite, is the causative agent of neosporosis, recognized as a major cause of bovine abortion around the world. Thailand is a developing agricultural country located in Southeast Asia. Livestock developments particularly in dairy cows of this country have been hampered by low productivity including milk and slow growth rate due to the impact of many pathogens including N. caninum. Currently, there is no effective method for control of neosporosis since there is less information regarding current status of infections. The objective of this study was to investigate the seroprevalence of neosporosis in dairy cows of the northern part of Thailand. During 2006–2007, the sera of 642 cows from 42 small farm holders with the top three highest consensus of dairy farms in the northern provinces, such as Chiang Rai, Chiang Mai and Lumpang were collected and performed tests. Antibodies to N. caninum were assayed by enzyme-linked immunosorbent assay (ELISA) with recombinant N. caninum surface antigen 1 (NcSAG1) and indirect fluorescent antibody test (IFAT). The overall prevalence of N. caninum infection in this study was 46.9% (301/642) by ELISA and 34.3% (220/642) by IFAT.

Keywords: Neospora caninum; dairy cows; NcSAG1; IFAT; Thailand

  • [1] Arunvipas P., Inpankaew T., Jittapalapong S. 2012a. Seroprevalence and Risk Factors of Neospora caninum Infection among Dairy Cows in the Western Provinces of Nakhon Pathom, Ratchaburi and Kanchanaburi, Thailand. Kasetsart Journal (Natural Science.), 46, 64–70. Google Scholar

  • [2] Arunvipas P., Inpankaew T., Jittapalapong S. 2012b. Risk factors of Neospora caninum infection in dogs and cats in dairy farms in Western Thailand. Tropical Animal Health and Production, 44, 1117–1121. DOI: 10.1007/s11250-011-0048-2. http://dx.doi.org/10.1007/s11250-011-0048-2CrossrefWeb of ScienceGoogle Scholar

  • [3] Atkinson R.A., Cook R.W., Reddacliff L.A., Rothwell J., Broady K.W., Harper P., Ellis J.T. 2000. Seroprevalence of Neospora caninum infection following an abortion outbreak in a dairy cattle herd. Australian Veterinary Journal, 78, 262–266. http://dx.doi.org/10.1111/j.1751-0813.2000.tb11752.xCrossrefGoogle Scholar

  • [4] Bae J.S., Kim D.Y., Hwang W.S., Kim J.H., Lee N.S., Nam H.W. 2000. Detection of IgG antibody against Neospora caninum in cattle in Korea. The Korean Journal of Parasitology, 38, 245–249. http://dx.doi.org/10.3347/kjp.2000.38.4.245CrossrefGoogle Scholar

  • [5] Chahan B., Gaturanga I., Xiaohong H., Liao M., Fukumoto S., Hirata H., Nishikawa Y., Suzuki H., Sugimoto C., Nagasawa H., Fujusaki K., Igarashi I., Mikami T., Xuan X. 2003. Serodiagnosis of Neospora caninum infection in cattle by enzyme-linked immunosorbent assay with recombinant truncated NcSAG1. Veterinary Parasitology, 118, 177–185. http://dx.doi.org/10.1016/j.vetpar.2003.10.010CrossrefGoogle Scholar

  • [6] Dubey J.P., Carpenter J.L., Speer A., Topper M.J., Uggla A. 1988. Newly recognized fatal protozoan disease of dogs. Journal of the American Veterinary Medical Association, 198, 1269–1285. Google Scholar

  • [7] Gondim L.F., Sartor I.F., Hasegawa M., Yamane I. 1999. Seroprevalence of Neospora caninum in dairy cattle in Bahia, Brazil. Veterinary Parasitology, 86, 71–75. http://dx.doi.org/10.1016/S0304-4017(99)00129-6CrossrefGoogle Scholar

  • [8] Hemphil A., Felleisen R., Connolly B., Gottstein B., Hentrich B., Muller N. 1997. Characterization of a cDNA-clone encoding Nc-p43, a major Neospora caninum tachyzoite surface protein. Parasitology, 115, 581–590. http://dx.doi.org/10.1017/S0031182097001650CrossrefGoogle Scholar

  • [9] Howe D.K., Crawford A.C., Lindsay D., Sibley L.D. 1998. The p29 and p35 immunodominant antigens of Neospora caninumtachyzoites are homologous to the family of surface antigens of Toxoplasma gondii. Infection and Immunity, 66, 5322–5328. Google Scholar

  • [10] Huong L.T.T., Ljungstrom B.L., Uggla A., Bjorkman C. 1998. Prevalence of antibodies to Neospora caninum and Toxoplasma gondii in cattle and water buffaloes in southern Vietnam. Veterinary Parasitology, 75, 53–57. http://dx.doi.org/10.1016/S0304-4017(97)00178-7CrossrefGoogle Scholar

  • [11] Jenkins M.C., Caver J.A., Björkman C., Anderson T.C., Romand S., Vinyard B., Uggla A., Thulliez P., Dubey J.P. 2000. Serological investigation of an outbreak of Neospora caninum-associated abortion in a dairy herd in southeastern United States. Veterinary Parasitology, 94, 17–26. http://dx.doi.org/10.1016/S0304-4017(00)00373-3CrossrefGoogle Scholar

  • [12] Jittapalapong S., Sangvaranond A., Inpankaew T., Phasuk C., Pinyopanuwat N., Chimnoi W., Kengradomkij C., Saengow S., Pumhom P., Arunwipat P., Anakewit T., Robertson I.D. 2008. Seroprevalence of Neospora caninum infections of dairy cows in the North-east of Thailand. Kasetsart Journal (Natural Science), 42, 61–66. Google Scholar

  • [13] Kashiwasaki Y., Pholpark S., Charoenchai A., Polsar C., Teeverapanya S., Pholpark M. 2001. Postnatal neosporosis in dairy cattle in northeast Thailand. Veterinary Parasitology, 94, 217–220. http://dx.doi.org/10.1016/S0304-4017(00)00358-7CrossrefGoogle Scholar

  • [14] Nishikawa Y., Kousaka Y., Tragoolpua K., Xuan X., Makala L., Fujisaki K., Mikami T., Nagasawa H. 2001. Characterization of Neospora caninum surface protein NcSRS2 based on Baculovirus expression system and its application for serodiagnosis of Neospora infection. Journal of Clinical Microbiology, 39, 3987–3991. http://dx.doi.org/10.1128/JCM.39.11.3987-3991.2001CrossrefGoogle Scholar

  • [15] Ooi H.K., Huang C.C., Yang C.H., Lee S.H. 2000. Serological survey and first finding of Neospora caninum in Taiwan, and the detection of its antibodies in various body fluids of cattle. Veterinary Parasitology, 90, 47–55. http://dx.doi.org/10.1016/S0304-4017(00)00211-9CrossrefGoogle Scholar

  • [16] Rodriguez I., Choromanski L., Rodgers S.J., Weinstock D. 2002. Survey of Neospora caninum antibodies in dairy and beef cattle from five regions of the United States. Veterinary Therapeutics, 3, 396–401. Google Scholar

  • [17] Suteeraparp P., Pholpark S., Pholpark M., Charoenchai A., Chompochan T., Yamane I., Kashiwazaki Y. 1999. Seroprevalence of antibodies to Neospora caninum and associated abortion in dairy cattle from central Thailand. Veterinary Parasitology, 86, 49–57. http://dx.doi.org/10.1016/S0304-4017(99)00130-2CrossrefGoogle Scholar

  • [18] Yu J., Xia Z., Liu Q., Liu J., Ding J., Zhang W. 2007. Seroepidemiology of Neospora caninum and Toxoplasma gondii in cattle and water buffaloes (Bubalus bubalis) in the People’s Republic of China. Veterinary Parasitology, 143, 79–85. http://dx.doi.org/10.1016/j.vetpar.2006.07.031Web of ScienceCrossrefGoogle Scholar

About the article

Published Online: 2014-05-15

Published in Print: 2014-06-01

Citation Information: Acta Parasitologica, Volume 59, Issue 2, Pages 305–309, ISSN (Online) 1896-1851, DOI: https://doi.org/10.2478/s11686-014-0245-z.

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© 2014 W. Stefański Institute of Parasitology, PAS. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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