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Acta Parasitologica

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Volume 59, Issue 2

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Seroprevalence of Neospora caninum in cats from the Czech Republic

Kamil Sedlák
  • Department of Virology and Serology, State Veterinary Institute Prague, Sidlistni 136/24, 165 03, Prague 6, Czech Republic
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/ Eva Bartova
  • Faculty of Veterinary, Department of Biology and Wildlife Diseases, Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Palackého tř. 1/3, 612 42, Brno, Czech Republic
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/ Tereza Machacova
  • Faculty of Veterinary, Department of Biology and Wildlife Diseases, Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Palackého tř. 1/3, 612 42, Brno, Czech Republic
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Published Online: 2014-05-15 | DOI: https://doi.org/10.2478/s11686-014-0246-y

Abstract

Sera of 414 cats coming from different parts of the Czech Republic were tested for N. caninum antibodies. Sera samples were collected during years 2002–2011. N. caninum antibodies were detected by a commercial competitive-inhibition enzyme-linked immunosorbent assay (cELISA) with cut off ≥30% inhibition. Samples positive in cELISA were confirmed by an indirect fluorescence antibody test (IFAT); titre ≥50 was considered positive. In total, 137 (33%) cats reacted positively in cELISA; N. caninum antibodies in IFAT were detected in 16 (3.86%) cats with titres 50 and 100. In 6 cats, positive for N. caninum antibodies, T. gondii antibodies were also detected by IFAT. It is the first report of N. caninum antibodies in domestic cats from the Czech Republic and third report in Europe.

Keywords: Neosporosis; antibodies; ELISA; IFAT; feline

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About the article

Published Online: 2014-05-15

Published in Print: 2014-06-01


Citation Information: Acta Parasitologica, Volume 59, Issue 2, Pages 359–361, ISSN (Online) 1896-1851, DOI: https://doi.org/10.2478/s11686-014-0246-y.

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© 2014 W. Stefański Institute of Parasitology, PAS. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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