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Volume 59, Issue 2


Seroprevalence of Neospora caninum in cats from the Czech Republic

Kamil Sedlák
  • Department of Virology and Serology, State Veterinary Institute Prague, Sidlistni 136/24, 165 03, Prague 6, Czech Republic
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/ Eva Bartova
  • Faculty of Veterinary, Department of Biology and Wildlife Diseases, Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Palackého tř. 1/3, 612 42, Brno, Czech Republic
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/ Tereza Machacova
  • Faculty of Veterinary, Department of Biology and Wildlife Diseases, Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Palackého tř. 1/3, 612 42, Brno, Czech Republic
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Published Online: 2014-05-15 | DOI: https://doi.org/10.2478/s11686-014-0246-y


Sera of 414 cats coming from different parts of the Czech Republic were tested for N. caninum antibodies. Sera samples were collected during years 2002–2011. N. caninum antibodies were detected by a commercial competitive-inhibition enzyme-linked immunosorbent assay (cELISA) with cut off ≥30% inhibition. Samples positive in cELISA were confirmed by an indirect fluorescence antibody test (IFAT); titre ≥50 was considered positive. In total, 137 (33%) cats reacted positively in cELISA; N. caninum antibodies in IFAT were detected in 16 (3.86%) cats with titres 50 and 100. In 6 cats, positive for N. caninum antibodies, T. gondii antibodies were also detected by IFAT. It is the first report of N. caninum antibodies in domestic cats from the Czech Republic and third report in Europe.

Keywords: Neosporosis; antibodies; ELISA; IFAT; feline

  • [1] Andre M.R., Adania C.H., Teixeira R.H.F., Silva K.F., Jusi M.M.G., Machado S.T.Z., De Bortolli C.P., Falcade M., Sousa L., Alegretti S.M., Felippe P.A.N., Machado R.Z. 2010. Antibodies to Toxoplasma gondii and Neospora caninum in captive neotropical and exotic wild canids and felids. Journal of Parasitology, 96, 1007–1009. DOI: 10.1645/GE-2502-1. http://dx.doi.org/10.1645/GE-2502.1CrossrefWeb of ScienceGoogle Scholar

  • [2] Arunvipas P., Inpankaew T., Jittapalapong S. 2011. Risk factors of Neospora caninum infection in dogs and cats in dairy farms in Western Thailand. Tropical Animal Health and Production, 44, 1117–1121. DOI: 10.1007/s11250-011-0048-2. http://dx.doi.org/10.1007/s11250-011-0048-2CrossrefWeb of ScienceGoogle Scholar

  • [3] Baszler T.V., Adams S., Vander-Schalie J., Mathison B.A., Kostovic M. 2001. Validation of a commercially available monoclonal antibody-based competitive-inhibition enzyme-linked immunosorbent assay for detection of serum antibodies to Neospora caninum in cattle. Journal of Clinical Microbiology, 39, 3851–3857. DOI: 10.1128/JCM.39.11.3851-3857.2001. http://dx.doi.org/10.1128/JCM.39.11.3851-3857.2001CrossrefGoogle Scholar

  • [4] Bresciani K.D.S., Gennari S.M, Serrano A.C.M., Ueno T, Franco L.G., Perri S.H.V. 2007. Antibodies to Neospora caninum and Toxoplasma gondii in domestic cats from Brazil. Parasitology Research, 100, 281–285. DOI: 10.1007/s00436-006-0262-4. http://dx.doi.org/10.1007/s00436-006-0262-4CrossrefWeb of ScienceGoogle Scholar

  • [5] Coelho W.M., Amarante A.F., Apolinario J.D.E., De Lima V.M., Perri S.H., Bresciani K.D. 2011. Seroepidemiology of Toxoplasma gondii and Neospora caninum and Leishmania spp. infection and risk factors for cats from Brazil. Parasitology Research, 109, 4. DOI: 10.1007/s00436-011-2461-x. http://dx.doi.org/10.1007/s00436-011-2461-xWeb of ScienceCrossrefGoogle Scholar

  • [6] Dubey J.P. 1992. A Review of Neospora caninum and Neospora-like infections in animals. Journal of Protozoology Research, 2, 40–52. Google Scholar

  • [7] Dubey J.P., Schares G. 2011. Neosporosis in animals-the last five years. Veterinary Parasitology, 180, 90–108. DOI: 10.1016/j.vetpar.2011.05.031. http://dx.doi.org/10.1016/j.vetpar.2011.05.031CrossrefWeb of ScienceGoogle Scholar

  • [8] Dubey J.P, Schares G., Ortega-Mora L.M. 2007. Epidemiology and control of neosporosis and Neospora caninum. Clinical Mikrobiology Reviews, 20, 323–367. DOI: 10.1128/CMR.00031-06. http://dx.doi.org/10.1128/CMR.00031-06CrossrefWeb of ScienceGoogle Scholar

  • [9] Ferroglio E., Guiso P., Pasino M., Accossato A., Trisciuoglio A. 2005. Antibodies to Neospora caninum in stray cats from north Italy. Veterinary Parasitology, 131, 31–34. DOI:10.1016/j.vetpar.2005.04.012. http://dx.doi.org/10.1016/j.vetpar.2005.04.012CrossrefGoogle Scholar

  • [10] Hamidine H., Mosalanejad B., Avizeh R., Hossein M., Ghorbanpour M., Namavari M. 2011. Neospora caninum and Toxoplasma gondii antibody prevalence in Ahvaz feral cats, Iran. Journal of Microbiology, 4, 217–222. Google Scholar

  • [11] Hornok S., Edelhofer R., Joachim A., Farkas R., Berta K., Repasi A., Lakatos B. 2008. Seroprevalence of Toxoplasma gondii and Neospora caninum infection of cats in Hungary. Acta Veterinaria Hungarica, 56, 81–88. DOI: 10.1556/AVet.56.2008.1.8. http://dx.doi.org/10.1556/AVet.56.2008.1.8CrossrefWeb of ScienceGoogle Scholar

  • [12] Millan J., Cabezon O., Pabon M., Dubey J.P., Almeria S. 2009a. Seroprevalence of Toxoplasma gondii and Neospora caninum in feral cats (Felis silvestris catus) in Malorca, Balearic Islands, Spain. Veterinary Parasitology, 165, 323–326. DOI: 10.1016/j.vetpar.2009.07.014. http://dx.doi.org/10.1016/j.vetpar.2009.07.014Web of ScienceCrossrefGoogle Scholar

  • [13] Millan J., Candela M.G., Palomares F., Cubero M.J., Rodriguez A., Barral M., De La Fuente J., Almeria S., Leon-Vizcaino L. 2009b. Disease threats to the endangered Iberian lynx (Lynx pardinus). Veterinary Journal, 182, 114–124. DOI: 10.1016/j.tvjl.2008.04.005. http://dx.doi.org/10.1016/j.tvjl.2008.04.005CrossrefWeb of ScienceGoogle Scholar

  • [14] Sedlak K., Bartova E. 2006. Seroprevalence of antibodies to Neospora caninum and Toxoplasma gondii in zoo animals. Veterinary Parasitology, 136, 223–231. DOI:10.1016/j.vetpar.2005.11.021. http://dx.doi.org/10.1016/j.vetpar.2005.11.021CrossrefGoogle Scholar

  • [15] Sobrino R., Dubey J.P., Pabon M., Linarez N., Kwok O.C., Millan J., Arnal M.C., Luco D.F., Lopez-Gatius F., Thulliez P., Gortazar C., Almeria S. 2008. Neospora caninum antibodies in wild carnivores from Spain. Veterinary Parasitology, 155, 190–197. DOI: 10.1016/j.vetpar.2008.05.009. http://dx.doi.org/10.1016/j.vetpar.2008.05.009CrossrefGoogle Scholar

  • [16] Spencer J.A., Higginbotham M.J., Blagburn B.L. 2003. Seroprevalence of Neospora caninum and Toxoplasma gondii in captive and free-ranging nondomestic felids in the United States. Journal of Zoo and Wildlife Medicine, 34, 246–249. DOI: http://dx.doi.org/10.1638/02-046. http://dx.doi.org/10.1638/02-046Google Scholar

About the article

Published Online: 2014-05-15

Published in Print: 2014-06-01

Citation Information: Acta Parasitologica, Volume 59, Issue 2, Pages 359–361, ISSN (Online) 1896-1851, DOI: https://doi.org/10.2478/s11686-014-0246-y.

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© 2014 W. Stefański Institute of Parasitology, PAS. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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