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Acta Parasitologica

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Volume 59, Issue 4 (Oct 2014)


The comparison of FLOTAC, FECPAK and McMaster techniques for nematode egg counts in cattle

Antonio Bosco
  • Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, CREMOPAR Regione Campania, Naples, Italy
  • Email:
/ Laura Rinaldi
  • Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, CREMOPAR Regione Campania, Naples, Italy
  • Email:
/ Maria Maurelli
  • Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, CREMOPAR Regione Campania, Naples, Italy
  • Email:
/ Vincenzo Musella
  • Department of Health Sciences, University Magna Graecia of Catanzaro, Catanzaro, Italy
  • Email:
/ Gerald Coles
  • School of Veterinary Sciences, University of Bristol, Langford House, Bristol, BS40 5DU, UK
  • Email:
/ Giuseppe Cringoli
  • Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, CREMOPAR Regione Campania, Naples, Italy
  • Email:
Published Online: 2014-09-20 | DOI: https://doi.org/10.2478/s11686-014-0282-7


Three methods, FLOTAC, FECPAK and McMaster were compared for accuracy and sensitivity for counting numbers of nematode eggs in faeces of naturally infected cattle with high or low nematode egg counts. Only FLOTAC gave positive results for 12 replicates from pooled samples with low egg counts making it more sensitive than FECPAK (67%) and McMaster (41.7%). FLOTAC resulted in generally higher egg counts and lower coefficients of variation than the other two methods used. The reliability of FECPAK and McMaster is depended on the area under the slide counted. All three methods can be used for making decisions whether to treat but FLOTAC or Mini-FLOTAC should be used for faecal egg count reduction tests when lower egg counts are present.

Keywords: FecPak; McMaster; FLOTAC; nematode egg count; cattle

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About the article

Published Online: 2014-09-20

Published in Print: 2014-10-01

Citation Information: Acta Parasitologica, ISSN (Online) 1896-1851, ISSN (Print) 1230-2821, DOI: https://doi.org/10.2478/s11686-014-0282-7.

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© 2014 W. Stefański Institute of Parasitology, PAS. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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