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Volume 59, Issue 4 (Oct 2014)


Gastrointestinal parasites in rural dogs and cats in Selangor and Pahang states in Peninsular Malaysia

Romano Ngui
  • Department of Parasitology, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia
  • Email:
/ Soo Lee
  • Department of Parasitology, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia
  • Email:
/ Nan Yap
  • Department of Parasitology, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia
  • Email:
/ Tiong Tan
  • Department of Parasitology, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia
  • Email:
/ Roslan Aidil
  • Department of Parasitology, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia
  • Email:
/ Kek Chua
  • Department of Biomedical Science, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia
  • Email:
/ Shafie Aziz
  • Department of Geography, Faculty of Arts and Social Sciences, University of Malaya, 50603, Kuala Lumpur, Malaysia
  • Email:
/ Wan Sulaiman
  • Department of Parasitology, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia
  • Email:
/ Arine Ahmad
  • Department of Parasitology, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia
  • Email:
/ Rohela Mahmud
  • Department of Parasitology, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia
  • Email:
/ Yvonne Lian
  • Department of Parasitology, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia
  • Email:
Published Online: 2014-09-20 | DOI: https://doi.org/10.2478/s11686-014-0306-3


To estimate the current prevalence of gastrointestinal (GI) parasites in dogs and cats, a total of 105 fresh faecal samples were collected from rural areas in Peninsular Malaysia. Each faecal sample was examined for the presence of GI parasites by microscopic examination after formalin-ether concentration technique and for protozoa, trichrome and Ziehl-Neelsen staining were employed. The overall prevalence of GI parasitic infection was 88.6% (95% CI = 82.5–94.7) in which 88.3% of dogs and 89.3% of cats were infected with at least one parasites species, respectively. There were 14 different GI parasites species (nematodes, cestodes and protozoa) detected, including Ancylostoma spp. (62.9%), Toxocara spp. (32.4%), Trichuris vulpis (21.0%), Spirometra spp. (9.5%), Toxascaris leonina (5.7%), Dipylidium caninum (4.8%), Ascaris spp. (2.9%), Hymenolepis diminuta (1.0%) and others. General prevalence of GI parasites showed a significant difference between helminth (84.4%) and protozoa (34.3%) infections. Monoparasitism (38.1%) was less frequent than polyparasitism (46.7%). As several of these GI parasites are recognized as zoonotic agents, the results of this investigation revealed that local populations may be exposed to a broad spectrum of zoonotic agents by means of environmental contamination with dogs and cats faeces and this information should be used to mitigate public health risks. Prevention and control measures have to be taken in order to reduce the prevalence rates especially in socioeconomically disadvantaged communities where animals live in close proximity to people, poor levels of hygiene and overcrowding together with a lack in veterinary attention and zoonotic awareness.

Keywords: Dogs; Cats; Gastrointestinal helminths and protozoa; rural area; zoonosis

  • [1] Bridger K.E., Whitney H. 2009. Gastrointestinal parasites in dogs from the Island of St. Pierre off the south coast of New foundland. Veterinary Parasitology, 162, 167–170. http://dx.doi.org/10.1016/j.vetpar.2009.02.016 [Web of Science] [Crossref]

  • [2] Bowman D.D., Montgomery S.P., Zajac A.M., Eberhard M.L., Kazacos K.R. 2010. Hookworms of dogs and cats as agents of cutaneous larva migrans. Trends Parasitology, 26, 162–167. http://dx.doi.org/10.1016/j.pt.2010.01.005 [Web of Science] [Crossref]

  • [3] Calvete C., Lucientes J., Castillo J.A., Estrada R., Gracia M.J., Peribanez M.A., Ferrer M. 1998. Gastrointestinal helminth parasites in stray cats from the mid-Ebro Valley, Spain. Veterinary Parasitology, 75, 235–240. http://dx.doi.org/10.1016/S0304-4017(97)00182-9 [Crossref]

  • [4] Claerebout E.S., Casaert A.C., Dalemans N., De Wilde N., Levecke B., Vercruysse J., Geurden T. 2009. Giardia and other intestinal parasites in different dog populations in Northern Belgium. Veterinary Parasitology, 161, 41–46. http://dx.doi.org/10.1016/j.vetpar.2008.11.024 [Web of Science] [Crossref]

  • [5] Conlan J.V., Sripa B., Attwood S., Newton P.N. 2011. A review of parasitic zoonoses in a changing Southeast Asia. Veterinary Parasitology, 182, 22–40. http://dx.doi.org/10.1016/j.vetpar.2011.07.013 [Crossref] [Web of Science]

  • [6] Despommier D. 2003. Toxocariasis: Clinical aspects, epidemiology, medical ecology, and molecular aspects. Clinical Microbiology Reviews, 16, 265–272. http://dx.doi.org/10.1128/CMR.16.2.265-272.2003 [Crossref]

  • [7] Eyles D.E., Jones F.E., Jumper J.R., Drinnon V.P. 1954. Amebic infection in dogs. Journal of Parasitology 40, 163–166. http://dx.doi.org/10.2307/3274296 [Crossref]

  • [8] Gracenea M., Mar Gómez M.S., Torres J. 2009. Prevalence of intestinal parasites in shelter dogs and cats in the metropolitan area of Barcelona (Spain). Acta Parasitologica, 54, 73–77. http://dx.doi.org/10.2478/s11686-009-0005-7 [Crossref] [Web of Science]

  • [9] Inpankaew T., Traub R., Thompson R.C., Sukthana Y. 2007. Canine parasitic zoonoses in Bangkok temples. Southeast Asian Journal of Tropical Medicine and Public Health, 38, 247–255.

  • [10] Katagiri S., Oliveira-Sequeira T.C.G. 2008. Prevalence of dog intestinal parasites and risk perception of zoonotic infection by dog owners in Sao Paulo State, Brazil. Zoonoses and Pubic Health, 55, 406–413. http://dx.doi.org/10.1111/j.1863-2378.2008.01163.x [Crossref] [Web of Science]

  • [11] Kirkpatrick C.E. 1990. Enteric protozoal infections, in greene ce (ed): Infectious diseases of the dog and cat. Philadelphia WB Saunders, 804-814.

  • [12] Lee C.C., Cheng N.A.B.Y., Bohari Y. 1993. Toxocara canis from domestic cats in Kuala Lumpur. Tropical Biomedicine, 10, 79–80.

  • [13] Lindsay D.S., Zajac A.M. 2004. Cryptosporidium infections in cats and dogs. Compendium on Continuing Education for the Practising Veterinarian, 26, 864–874.

  • [14] Lorenzini G., Tasca T., De Carli G.A. 2007. Prevalence of intestinal parasites in dogs and cats under veterinary care in Porto Alegre, Rio Grande do Sul, Brazil. Brazilian Journal of Veterinary Research and Animal Science, 44, 137–145.

  • [15] McGlade T.R., Robertson I.D., Elliot A.D., Read C., Thompson R.C. 2003. Gastrointestinal parasites of domestic cats in Perth, Western Australia. Veterinary Parasitology, 117, 251–262. http://dx.doi.org/10.1016/j.vetpar.2003.08.010 [Crossref]

  • [16] Mahdy M.A., Lim Y.A.L., Ngui R., Siti Fatimah M.R., Choy S.H., Yap N.J., Al-Mekhlafi H.M., Jamaiah I., Johari S. 2012. Prevalence and zoonotic potential of canine hookworms in Malaysia. Parasites and Vectors, 5, 88. http://dx.doi.org/10.1186/1756-3305-5-88 [Crossref]

  • [17] Martinez-Moreno F.J., Hernandez S., Lopez-Cobos E., Becerra C., Acosta I., Martinez-Moreno A. 2007. Estimation of canine intestinal parasites in Cordoba (Spain) and their risk to public health. Veterinary Parasitology, 143, 7–13. http://dx.doi.org/10.1016/j.vetpar.2006.08.004 [Crossref] [Web of Science]

  • [18] Mohd Zain S.N. 2010. Comparative study of the macroparasite communities of stray cats from four urban cities in Peninsular Malaysia. Proceedings of the Fourth ASEAN Congress of Tropical Medicine and Parasitology 2010.

  • [19] Ng J., Eastwood K., Durrheim D., Massey P., Walker B., Armson A., Ryan U. 2008. Evidence supporting zoonotic transmission of Cryptosporidium in rural New South Wales. Experimental Parasitology, 119, 192–195. http://dx.doi.org/10.1016/j.exppara.2008.01.010 [Web of Science] [Crossref]

  • [20] Ngui R., Saidon I., Chow S.C., Rohela M., Lim YAL. 2011. Prevalence and risk factors of intestinal parasitism in rural and remote West Malaysia. PLoS Neglected Tropical Diseases, 5, e974. http://dx.doi.org/10.1371/journal.pntd.0000974 [Crossref] [Web of Science]

  • [21] Ngui R., Lim Y.A.L., Traub R., Mahmud R., Mistam M.S. 2012. Epidemiological and genetic data supporting the transmission of Ancylostoma ceylanicum among human and domestic animals. PLoS Neglected Tropical Diseases, 6, e1522. http://dx.doi.org/10.1371/journal.pntd.0001522 [Crossref] [Web of Science]

  • [22] Oliveira-Sequeira T.C., Amarante A.F., Ferrari T.B., Nunes L.C. 2002. Prevalence of intestinal parasites in dogs from Sao Paulo State, Brazil. Veterinary Parasitology, 103, 19–27. http://dx.doi.org/10.1016/S0304-4017(01)00575-1 [Crossref]

  • [23] Overgaauw P.A.M., van Zutphen L., Hoek D., Yaya F.O., Roelfsema J., Pinelli E., van Knapen F., Kortbeek L.M. 2009. Zoonotic parasites in fecal samples and fur from dogs and cats in the Netherlands. Veterinary Parasitology, 163, 115–122. http://dx.doi.org/10.1016/j.vetpar.2009.03.044 [Web of Science] [Crossref]

  • [24] Papazahariadou M., Founta A., Papadopoulos E., Chilounakis S., Antoniadou-Sotiriadou K., Theodorides Y. 2007. Gastrointestinal parasites of shepherd and hunting dogs in the Serres Prefecture, Northern Greece. Veterinary Parasitology, 148, 170–173. http://dx.doi.org/10.1016/j.vetpar.2007.05.013 [Web of Science] [Crossref]

  • [25] Paquet-Durand I., Hernandez J., Dolz G., Zuniqa J.J., Schneider T., Epe C. 2007. Prevalence of Toxocara spp., Toxascaris leonina and ancylostomidae in public parks and beaches in different climate zones of Costa Rica. Acta Tropica, 104, 30–37. http://dx.doi.org/10.1016/j.actatropica.2007.06.011 [Crossref] [Web of Science]

  • [26] Prociv P., Croese J. 1990. Human eosinophilic enteritis caused by dog hookworm Ancylostoma-caninum. Lancet, 335, 1299–1302. http://dx.doi.org/10.1016/0140-6736(90)91186-E [Crossref]

  • [27] Robertson I.D., Irwin P.J., Lymbery A.J., Thompson R.C. 2000. The role of companion animals in the emergence of parasitic zoonoses. International Journal for Parasitology, 30, 1369–1377. http://dx.doi.org/10.1016/S0020-7519(00)00134-X [Crossref]

  • [28] Schantz P.M. 1994. Of worms, dogs, and human hosts: Continuing challenges for veterinarians in prevention of human disease. Journal of the American Veterinary Medical Association, 204, 1023–1028.

  • [29] Scorza A.V., Duncan C., Miles L., Lappin M.R. 2011. Prevalence of selected zoonotic and vector-borne agents in dogs and cats in Costa Rica. Veterinary Parasitology, 183, 178–183. http://dx.doi.org/10.1016/j.vetpar.2011.06.025 [Crossref] [Web of Science]

  • [30] Shanta C.S., Wan S.P., Kwong K.H. 1980. A survey of the endo and ectoparasites of cats in and around Ipoh, West Malaysia. Malay. Veterinary Journal, 7, 17–27.

  • [31] Shimada A., Muraki Y., Awakura T., Umemura T., Sanekata T., Kuroki T., Ishihara M. 1992. Necrotic colitis associated with Entamoeba histolytica infection in a cat. Journal of Comparative Pathology, 106, 195–199. http://dx.doi.org/10.1016/0021-9975(92)90048-Y [Crossref]

  • [32] Sommerfelt I.E., Cardillo N., Lopez C., Ribicich M., Gallo C., Franco A. 2006. Prevalence of Toxocara cati and other parasites in cats’ faeces collected from the open spaces of public institutions: Buenos Aires, Argentina. Veterinary Parasitology, 140, 296–301. http://dx.doi.org/10.1016/j.vetpar.2006.03.022 [Crossref]

  • [33] Soriano S.V., Pierangeli N.B., Roccia I., Bergagna H.F., Lazzarini L.E., Celescinco A., Saiz M.S., Kossman A., Contreras P.A., Arias C., Basualdo J.A. 2010. A wide diversity of zoonotic intestinal parasites infects urban and rural dogs in Neuquen, Patagonia, Argentina. Veterinary Parasitology, 167, 81–85. http://dx.doi.org/10.1016/j.vetpar.2009.09.048 [Web of Science] [Crossref]

  • [34] Traub R.J., Inpankaew T., Sutthikornchai C., Sukthana Y., Thompson R.C. 2008. PCR-based coprodiagnostic tools reveal dogs as reservoirs of zoonotic ancylostomiasis caused by Ancylostoma ceylanicum in temple communities in bangkok. Veterinary Parasitology, 155, 67–73. http://dx.doi.org/10.1016/j.vetpar.2008.05.001 [Crossref] [Web of Science]

  • [35] Traub R.J., Robertson I.D., Irwin P., Mencke N., Monis P., Thompson R.C.A. 2003. Humans, dogs and parasitic zoonoses — Unravelling the relationships in a remote endemic community in Northeast India using molecular tools. Parasitology Research, 90, S156–S157. http://dx.doi.org/10.1007/s00436-003-0925-3 [Crossref]

  • [36] Traub R.J., Robertson I.D., Irwin P.J., Mencke N., Thompson R.C.A. 2005. Canine gastrointestinal parasitic zoonoses in India. Trends Parasitology, 21, 42–48. http://dx.doi.org/10.1016/j.pt.2004.10.011 [Crossref]

  • [37] Wittnich C. 1976. Entamoeba histolytica infection in a German shepherd dog. Canadian Veterinary Journal, 17, 259–263.

  • [38] Xiao L., Fayer R., Ryan U., Upton S.J. 2004. Cryptosporidium taxonomy: Recent advances and implications for public health. Clinical Microbiologyl Reviews, 17, 72–97. http://dx.doi.org/10.1128/CMR.17.1.72-97.2004 [Crossref]

  • [39] Yoshida Y., Okamoto K., Matsuo K., Kwo E.H., Retnasabapathy A. 1973. The occurrence of Ancylostoma braziliense (de Faria, 1910) and Ancylostoma ceylanicum (Looss, 1911) in Malaysia. Southeast Asian Journal of Tropical Medicine and Public Health, 4, 498–503.

  • [40] Zhu X.Q., Gasser R.B., Jacobs D.E., Hung G.C., Chilton N.B. 2000. Relationships among some ascaridoid nematodes based on ribosomal DNA sequence data. Parasitology Research, 86, 738–744. http://dx.doi.org/10.1007/PL00008561 [Crossref]

About the article

Published Online: 2014-09-20

Published in Print: 2014-10-01

Citation Information: Acta Parasitologica, ISSN (Online) 1896-1851, ISSN (Print) 1230-2821, DOI: https://doi.org/10.2478/s11686-014-0306-3. Export Citation

© 2014 W. Stefański Institute of Parasitology, PAS. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. (CC BY-NC-ND 3.0)

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