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Acta Parasitologica

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Volume 60, Issue 1


The pathogenic role of Blastocystis isolated from patients with irritable bowel syndrome and colitis from Iasi, Romania

Doina S. Matiut
  • Corresponding author
  • Praxis Laboratory, Bd. Independentei, No. 33, 700102, Iasi, Romania
  • Laboratory of Diagnosis and Investigation in Public Health, Str. Nicolae Balcescu No. 21, Iasi, Romania
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Lucian Hritcu
  • Department of Biology, Alexandru Ioan Cuza University, Bd. Carol I, No.11, Iasi 700506, Romania
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2014-12-30 | DOI: https://doi.org/10.1515/ap-2015-0015


Blastocystis is a common parasite and regarded as one of the etiologic agents of irritable bowel syndrome, colitis and chronic diarrhea. Our study was undertaken in order to identify different subtypes of Blastocystis isolated in patients with irritable bowel syndrome and colitis, as well as with chronic diarrhea and to evaluate their pathogenic potential. Seventy-three subjects (10 asymptomatic infected subjects, 49 subjects harboring Blastocystis or associated with other etiologic agents like bacteria, yeasts, protozoa, helminthes and 14 subjects with unknown etiologic agents) were investigated by in vitro parasitological and bacteriological stool samples followed by PCR subtyping of Blastocystis using STS primers, immunological markers (total serum IgA and IgE antibody levels), Helicobacter pylori antigen rapid test and fecal occult blood test. Also, among 49 subjects, there were 12 subjects harboring Blastocystis as the single etiologic agent. Subtyping proved that only three subtypes of Blastocystis were identified as following: subtype II (66.66%) in single infected subjects, subtype I (16.66%) in mixed infection with subtype II and subtype IV (8.33%) in single infected subjects. Total serum IgA and IgE antibody levels were in normal range. Subtype II was the most frequent subtype followed by subtype I and subtype IV of Blastocystis isolates in patients with irritable bowel syndrome, colitis, and chronic diarrhea as well as in asymptomatic infected group. Our results suggest that the severity of clinical manifestations depend on factors involving the host and possible parasitic density and not necessarily by isolated subtype.

Keywords: Blastocystis subtype; host; irritable bowel syndrome; colitis; chronic diarrhea


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About the article

Received: 2014-03-06

Revised: 2014-09-10

Accepted: 2014-09-19

Published Online: 2014-12-30

Published in Print: 2014-03-01

Citation Information: Acta Parasitologica, Volume 60, Issue 1, Pages 116–123, ISSN (Online) 1896-1851, DOI: https://doi.org/10.1515/ap-2015-0015.

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