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Acta Parasitologica

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Volume 60, Issue 2


Antiparasitic DNA vaccines in 21st century

Halina Wedrychowicz
  • Corresponding author
  • Department of Molecular Biology, Laboratory of Molecular Parasitology, W. Stefański Institute Parasitology, Polish Academy of Sciences, 51/55 Twarda St., 00-818 Warsaw, Poland
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Published Online: 2015-03-25 | DOI: https://doi.org/10.1515/ap-2015-0026


Demands for effective vaccines to control parasitic diseases of humans and livestock have been recently exacerbated by the development of resistance of most pathogenic parasites to anti-parasitic drugs. Novel genomic and proteomic technologies have provided opportunities for the discovery and improvement of DNA vaccines which are relatively easy as well as cheap to fabricate and stable at room temperatures. However, their main limitation is rather poor immunogenicity, which makes it necessary to couple the antigens with adjuvant molecules. This paper review recent advances in the development of DNA vaccines to some pathogenic protozoa and helminths. Numerous studies were conducted over the past 14 years of 21st century, employing various administration techniques, adjuvants and new immunogenic antigens to increase efficacy of DNA vaccines. Unfortunately, the results have not been rewarding. Further research is necessary using more extensive combinations of antigens; alternate delivery systems and more efficient adjuvants based on knowledge of the immunomodulatory capacities of parasitic protozoa and helminths.

Keywords: DNA vaccines; adjuvants; exprimental trials; immunomodulation


  • Ahmad G., Torren W., Zhang W., Wyatt M., Siddiqui A.A. 2009a. Sm-p80-based DNA vaccine formulation induces potent protective immunity against Schistosoma mansoni. Parasite Immunology, 31, 156-161. DOI: 10.1111/j.1365-3024.2008.001091.x CrossrefPubMedGoogle Scholar

  • Ahmad G., Zhang W., Torben W., Damian R.T., Chavez-Suarez M., Wolf R.F,. White G.L. 2009b. Protective and antifecundity effects of Sm-p80-based DNA vaccine formulation against Schistosoma mansoni in a nonhuman primate model. Vaccine, 27, 2830-2837. DOI: 10.1016/j.vaccine.2009.02.096CrossrefGoogle Scholar

  • Ahmad G., Zhang W., Torben W., Haskins C., Diggs S., Noor Z., Le L., Siddiqui A.A. 2009c Prime/boost and recombinant protein vaccination strategies using Sm-p80 protects against Schistosoma mansoni infection in the mouse model to levels previously attainable only by the irradiated cercarial vaccine. Parasitology Research, 105, 1767-1777. DOI: 10.1007/s00 436-009-1646-z CrossrefGoogle Scholar

  • Anand S.B., Murugan V., Prabhu P.R., Anandharaman V., Reddy M.V., Kaliraj P. 2008. Comparison of immunogenicity, protective efficacy of single and cocktail DNA vaccine of Brugia malayi abundant larval transcript (ALT-2) and thioredoxin peroxidase (TPX) inmice. Acta Tropica, 107, 106-112Google Scholar

  • Anand S.B., Kodumudi K.N., Reddy M.V., Kaliraj P. 2011. A combination of two Brugia malayi filarial vaccine candidate antigens (BmALT-2 and BmVAH) enhances immune responses and protection in jirds. Journal of Helminthology, 85, 442-452. DOI: org/10.1017/S0022149X10000799CrossrefGoogle Scholar

  • Bergman P.J., Camps-Palau M.A., McKnight J.A., Leibman N.F., Craft D.M., Leung C., Liao J., Riviere I., Sadelain M., Hohenhaus A.E., Gregor P., Houghton A.N., Perales M.A., Wolchok J.D. 2006. Development of a xenogenetic DNA vaccine program for canine malignantmelanoma at the Animal Medical Center. Vaccine, 24, 4582-4585. DOI: 10.1016/j.vaccine.2005.08.027CrossrefGoogle Scholar

  • Bolhassani A., Gholami E., Zahedifard F., Moradin N., Parsi P., Doustdari F., Seyed N., Papadopoulou B., Rafati S. 2011. Leishmania major: Protective capacity of DNA vaccine using amastin fused to HSV-1 VP22 and EGFP in BALB/c mice model. Experimental Parasitology, 128, 9-17. DOI: 10.1016/ j.exppara.2011.01.012CrossrefGoogle Scholar

  • Cao S., Mousa A.A., Aboge G.O., Kamyingkird K., Zhou M., Moumouni P.F.A., Terkawi M.A., Masatani T., Nishikawa Y., Suzuki H., Fukumoto S., Xuan X. 2013. Prime-boost vaccinationwith plasmid DNA followed by recombinant vaccinia virus expressing BgGARP induced a partial protective immunity to inhibit Babesia gibsoni proliferation in dogs. Acta Parasitologica, 58, 619-623. DOI: 10.2478/s11686-013-0183-1CrossrefGoogle Scholar

  • Cannas A., Naguleswaran A., Muller N., Eperon S., Gottstein B., Hemphill A. 2003. Vaccination of mice against experimental Neospora caninum infection using NcSAG1- and NcSRS2- based recombinant antigens and DNA vaccines. Parasitology, 126, 303-312Google Scholar

  • Chlichlia K., Bahgat M., Ruppel A., Schirrmacher V. 2001. DNA vaccination with asparaginyl endopeptidase (Sm32) from the parasite Schistosoma mansoni: anti-fecundity effect induced in mice. Vaccine, 20, 439-447CrossrefGoogle Scholar

  • Cruz Lança A.S., de Sousa K.P., Atouguia J., Prazeres D.M.F., Monteiro G.A., Silva M.S. 2011. Trypanosoma brucei: Immunisation with plasmid DNA encoding invariant surface glycoprotein gene is able to induce partial protection in experimental African trypanosomiasis. Experimental Parasitology, 127, 18-24. DOI: 10.1016/j.exppara.2010.06.017CrossrefGoogle Scholar

  • Da’dara A.A., Skelly P.J., Fatakawadala M., Visovatti S., Eriksson E., Harn D.A. 2002. Comparative efficacy of the Schistosoma mansoni nucleic acid vaccine, Sm23, following microseeding or gene gun delivery. Parasite Immunology, 24, 179-187Google Scholar

  • Da’Dara A.A., Li Y.S., Xiong T., Zhou J.,Williams G.M., McManus D.P., Fenge Z., Yu X.L., Gray D.J., Harn D.A. 2008. DNAbased vaccines protect against zoonotic schistosomiasis in water buffalo. Vaccine, 26, 3617-3625. DOI: 10.1016/j.vaccine.2008.04.080CrossrefGoogle Scholar

  • Dalton J.P., Robinson M.W., Mulcahy G., O’Neill S.M., Donnelly S. 2013. Immunomodulatory molecules of Fasciola hepatica. Candidates for both vaccine and immunotherapeutic development. Veterinary Parasitology, 195, 272-285. DOI: org/10.1016/j.vetpar.2013.04.008CrossrefGoogle Scholar

  • Davidson A.H., Traub-Dargatz J.L., Rodeheaver R.M., Ostlund E.N., Pedersen D.D., Moorhead R.G., Stricklin J.B., Dewell R.D., Roach S.D., Long R.E., Albers S.J., Callan R.J., Salman M.D.2005. Immunologic responses to West Nile virus in vaccinated and clinically affected horses. Journal of the American Veterinary Medical Association, 226, 240-245. DOI: 10.2460/ javma.2005.226.240CrossrefGoogle Scholar

  • Dobano C., Rogers W.O.,Gowda K., Doolan D.L. 2007. Targeting antigen to MHC Class I and Class II antigen presentation pathways for malaria DNA vaccines. Immunology Letters, 111, 92-102. DOI: 10.1016/j.imlet.2007.05.007CrossrefGoogle Scholar

  • Du A., Wang S. 2005. Efficacy of a DNA vaccine delivered in attenuated Salmonella typhimurium against Eimeria tenella infection in chickens. International Journal forParasitology, 35, 777-785. DOI: 10.1016/j.ijpara.2005.03.005CrossrefGoogle Scholar

  • Dunachie S.J.,Walther M., Epstein J.E., Keating S., Berthoud T., Andrews, L., Andersen R.F., Bejon P., Goonetilleke N., Poulton I., Webster D.P., Butcher G., Watkins K., Sinden R.E., Levine GL., Richie, T.L., Schneider J., Kaslow D., Gilbert S.C., Carucci D.J., Hill A.V.S. 2006. A DNA Prime-Modified Vaccinia Virus Ankara Boost Vaccine Encoding Thrombospondin- Related Adhesion Protein but Not Circumsporozoite Protein Partially Protects Healthy Malaria- Naive Adults against Plasmodium falciparum Sporozoite Challenge. Infection and Immunity, 74, 5933-5942. DOI: 10.1128/IAI.00590-06CrossrefGoogle Scholar

  • Espino A.M., Morales A., Delgado B., Rivera F.M., Figueroa O., Suárez E. 2010. Partial immunity to Fasciola hepatica in mice after vaccination with FhSAP2 delivered as recombinant protein or DNA construct. Ethnicity & Disease, 20, S1-17-23Google Scholar

  • Fukumoto F., Tamaki Y., Igarashi I., Suzuki H., Xuan X. 2009. Immunogenicity and growth inhibitory efficacy of the prime- boost immunization regime with DNA followed by recombinant vaccinia virus carrying the P29 gene of Babesia gibsoni in dogs. Experimental Parasitology, 123, 296-301. DOI: 10. 1016/j.exppara.2009.08.012CrossrefGoogle Scholar

  • Garver K.A., LaPatra S.E., Kurath G. 2005. Efficacy of an infectious hematopoietic necrosis (IHN) virus DNA vaccine in Chinook Oncorhynchus tshawytscha and sockeye O. nerka salmon. Diseases of Aquatic Organisms, 64, 13-22. DOI: 10.3354/ dao064013CrossrefGoogle Scholar

  • Geriletu Xu L., Xurihu Li X. 2011. Vaccination of chickens with DNA vaccine expressing Eimeria tenella MZ5-7 against coccidiosis. Veterinary Parasitology, 177, 6-12. DOI: 10.1016/ j.vetpar.2010.11.041CrossrefGoogle Scholar

  • Han K., Xu L., Yan R., Song X., Li X. 2012. Vaccination of goats with glyceraldehyde-3- phosphate dehydrogenase DNA vaccine induced partial protection against Haemonchus contortus. Veterinary Immunology and Immunopathology, 149, 177-185. DOI :10.1016/j.vetimm.2012.06.016. Epub 2012 Jun 19CrossrefGoogle Scholar

  • He S., Yang L., Lv Z., Hu W., Cao J., Wei J., Sun X., Zheng H., Wu Z. 2010. Molecular and functional characterization of a mortalin-like protein from Schistosoma japonicum (SjMLP/ hsp70) as a member of the HSP70 family. Parasitology Research, 107, 955-966. DOI: 10.1007/s00436-010-1960-5CrossrefGoogle Scholar

  • Hezarjaribi H.Z., Ghaffarifar F., Dalimi F., Sharifi Z., Jorjani O. 2013. Effect of IL-22 on DNA vaccine encoding LACK gene of Leishmania major in BALB/c mice. Experimental Parasitology, 134, 341-348. DOI: org/10.1016/j.exppara.2013.03.012CrossrefGoogle Scholar

  • Hiszczynska-Sawicka E., Olędzka G., Holec-Gąsior L., Li H., Xua, J.B. Sedcole R., Kur J., Bickerstaffe R., Stankiewicz M. 2011a. Evaluation of immune responses in sheep inducedby DNA immunization with genes encoding GRA1, GRA4, GRA6 and GRA7 antigens ofToxoplasma gondii. Veterinary Parasitology, 177, 281-289. DOI: 10.1016/j.vetpar.2010. 11.047 CrossrefGoogle Scholar

  • Hiszczynska-Sawicka E., Li H., Xua J.B., Holec-Gąsior L., Kur J., Sedcole R., Bickerstaffe R., Stankiewicz M. 2011b. Modulation of immune response to Toxoplasma gondii in sheep by immunization with a DNA vaccine encoding ROP1 antigen as a fusion protein with ovine CD154. Veterinary Parasitology, 183, 72-78. DOI: 10.1016/j.vetpar.2011.06.010CrossrefGoogle Scholar

  • Januszkiewicz K. 2010. Optimalization of vaccination against Fasciola hepatica infections using cDNA encoding for selected fluke antigens and specific for the host species CTLA-4 molecule. PhD Thesis. Witold Stefański Institute of Parasitology Polish Academy of Sciences, Warsaw, Poland Google Scholar

  • Jaros S., Jaros D., Wesolowska A., Zygner W., Wedrychowicz H. 2010. Blocking Fasciola hepatica‘s energy metabolism - a pilot study of vaccine potential of a novel gene - phosphoglycerate kinase. Veterinary Parasitology, 172, 229-237. DOI: 10.1016/j.vetpar.2010.05.008CrossrefGoogle Scholar

  • Jayaraj R., Piedrafita D., Spithill T., Smooker P. 2012. Evaluation of the immune responses induced by four targeted DNA vaccines encoding the juvenile liver fluke antigen, cathepsin B in a mouse model. Genetic Vaccines and Therapy 2012, 10, 2-7, http://www.gvt-journal.com/content/10/1/7Google Scholar

  • Jenkins M., Parker C., Tuo W.,Vinyard B., Dubey J.P. 2004. Inclusion of CpG adjuvant with plasmid DNA coding for NcGRA7 improves protection against congenital neosporosis. Infection and Immunity, 72, 1817-1819, DOI: 10.1128/IAI.72.3.1817-1819.2004CrossrefGoogle Scholar

  • Kalyanasundaram R., Balumuri P. 2011. Multivalent vaccine formulation with BmVAL-1 and BmALT-2 confer significant protection against challenge infections with Brugia malayi in mice and jirds. Research and Reports in Tropical Medicine 2011, 45-56. DOI: 10.2147/RRTMS13679CrossrefGoogle Scholar

  • Kennedy N.J., Spithill T.W., Tennent J., Wood P.R., Piedrafita D. 2006. DNA vaccines in sheep: CTLA-4 mediated targeting and CpG motifs enhance immunogenicity in a DNA prime/ protein boost strategy. Vaccine, 24, 970-979. DOI: 10.1016/ j.vaccine.2005.08.076CrossrefGoogle Scholar

  • Kimani D., Jagne Y.J., Cox M., Kimani E., Bliss C.M., Gitau, E., Ogwang C., Afolabi M.O., Bowyer G., Collins K.A. et al. 2014. Translating the immunogenicity of prime-boost immunization with ChAd63 and MVA ME-TRAP from malaria naive to malaria-endemic populations. Molecular Therapy, 22, 1992-2003. DOI: 10.1038/mt.2014.109.CrossrefGoogle Scholar

  • Klinman D.M., Yamshchikov G., Ishigatsubo Y. Contribution of CpG motifs to the immunogenicity of DNA vaccines. Journal of Immunology, 158, 3635-3642Google Scholar

  • Kofta W., Wędrychowicz H. 2001. c-DNA vaccination against parasitic infections: advantages and disadvantages. Veterinary Parasitology 94; 243-247, DOI: 10.1016/SO304-4017(01) 00478-2CrossrefGoogle Scholar

  • Kurup S.P., Tewari A.K. 2012. Induction of protective immune response in mice by a DNA vaccine encoding Trypanosoma evansi beta tubulin gene. Veterinary Parasitology, 187, 9-16. DOI: 10.1016/j.vetpar.2012.01.009CrossrefGoogle Scholar

  • Lee J.S., Kim I.S., Sohn W.M., Lee J., Yong T.S. 2006a. A DNA vaccine encoding a fatty acid binding protein of Clonorchis sinensis induces protective immune response in Sprague-Dawley rats. Scandinavian Journal of Immunology, 63, 169-176 DOI: 10.1111/j.1365-3083.2006.01721.x CrossrefGoogle Scholar

  • Lee J., Kim I.S., Sohn W., Lee J., Yong T. 2006.b Vaccination with DNA encoding cysteine proteinase confers protective immune response to rats infected with Clonorchis sinensis.Vaccine, 24, 2358-2366CrossrefGoogle Scholar

  • Levine M.M. and Sztein M.B. 2004. Vaccine development strategies for improving immunization: The role of modern immunology. Nature Immunology, 5, 460-464. DOI: 10.1038/ni0504-460CrossrefGoogle Scholar

  • Li M., Lei J., WangT., Lu S., Guan F., Liu W., Li Y. 2011. Cimetidine enhances the protective effect of GST DNA vaccine against Schistosoma japonicum. Experimental Parasitology 128, 427-432. DOI: 10.1016/j.exppara.2011.05.012CrossrefGoogle Scholar

  • Liddell S., Parker C., Vinyard B., Jenkins M., Dubey J.P. 2003. Immunization of mice with plasmid DNA coding for NcGRA7 or NcsHSP33 confers partial protection against vertical transmission of Neospora caninum. Journal of Parasitology, 89, 496-500. DOI: org/10.1645/GE-2969CrossrefGoogle Scholar

  • Liu, M.A. 2010. DNA vaccines: An historical perspective and view to the future. Immunological Reviews, 239, 62-84. DOI: org/10.1111/j.1600-065X.2010.00980.x Liu Q., Shang L., Jin H., WeiF., Zhu Q., Gao H. 2010. The protective effect of a Toxoplasma gondii SAG1 plasmid DNA vaccine in mice is enhanced with IL-18. Research in Veterinary Science, 89, 93-97. DOI: 10.1016/j.rvsc.2010.01.007CrossrefGoogle Scholar

  • Mancini-Bourgine M., Fontaine H., Brechot C., Pol S., Michel M.L. 2006. Immunogenicity of a hepatitis B DNA vaccine administered to chronic HBV carriers. Vaccine, 24, 4482-4489. DOI: 10.1016/j.vaccine.2005.08.013CrossrefGoogle Scholar

  • Masih S., Arora S.K., Vasishta R.K. 2011. Efficacy of Leishmania donovani ribosomal P1 gene asDNA vaccine in experimental visceral leishmaniasis. Experimental Parasitology, 129, 55-64. DOI: 10.1016/j.exppara.2011.05.014CrossrefGoogle Scholar

  • Martin J.E., Louder M.K., Holman L.A., Gordon I.J., Enama M.E, Larkin B.D. et al. 2008 A SARS DNA vaccine induces neutralizing antibody and cellular immune responses in healthy adults in a Phase I clinical trial. Vaccine, 26, 6338-6343. DOI: 10.1016/j.vaccine.2008.09.026CrossrefGoogle Scholar

  • McCullers J.A. 2007. Evolution, benefits, and shortcomings of vaccine management. Journal of Managed Care Pharmacy, 13, S2-S6Google Scholar

  • Mc Neilly T.N., Nisbet A.J. 2014. Immune modulation by helminth parasites of ruminants: implications for vaccine development and host immune competence. Parasite, 21, 51-62. DOI: 10.1051/parasite/2014051CrossrefGoogle Scholar

  • Molloy M., Bouladoux N., Belkaid Y. 2012. Intestinal microbiota: shaping local and systemic immune responses. Seminar in Immunology, 24, 51-55. DOI: 10.1016/j.smim.2011CrossrefGoogle Scholar

  • Nielsen H.V., Di Cristina M., Beghetto E., Spadoni A., Petersen E., Gargano N. 2006. Toxoplasma gondii: DNA vaccination with bradyzoite antigens induces protective immunity in mice against oral infection with parasite cysts. Experimental Parasitology, 112, 274-279. DOI: 10.1016/j.exppara.2005.11.009CrossrefGoogle Scholar

  • Payette P.J., Weeratna R.D., McCluskie M.J., Davis H.L. 2001. Immune- mediated destruction of transfected myocytes following DNA vaccination occurs via multiple mechanisms. Gene Therapy, 8, 1395-1400. DOI: org/10.1038/sj.gt.3301534CrossrefGoogle Scholar

  • Person R., Bodles-Brakhop A.M., Pope M.A., Brown P.A., Khan A.S., Draghia-Akli R. 2008. Growth hormone-releasing hormone plasmid treatment by electroporation decreases offspring mortality over three pregnancies. Molecular Therapy, 16, 1891-1897. DOI: 10.1038/mt.2008.178CrossrefGoogle Scholar

  • Qu D., Han J., Du A. 2013. Evaluation of protective effect of multiantigenic DNA vaccine encoding MIC3 and ROP18 antigen segments of Toxoplasma gondii in mice Parasitology Research, 112, 2593-2599. DOI 10.1007/s00436-013-3425-0CrossrefGoogle Scholar

  • Rafati S., Zahedifard F., Azari M.K.,Taslimi Y., Taheri T. 2008. Leishmania infantum: Prime boost vaccination with C-terminal extension of cysteine proteinase type I displays both type 1 and 2 immune signatures in BALB/c mice. Experimental Parasitology, 118, 393-401. DOI: 10.1016/j.exppara.2007.10.004CrossrefGoogle Scholar

  • Rainczuk A., Scorza T., Spithill T.W., Smooker P.M. 2004. A bicistronic DNA vaccine containing Apical Membrane Antigen 1 and Merozoite Surface Protein 4/5 can prime humoral and cellular immune responses and partially protect mice against virulent Plasmodium chabaudi adami DS malaria.. Infection and Immunity, 72, 5565-5573. DOI: 10.1128/IAI.72.10. 5565-5573.2004CrossrefGoogle Scholar

  • Rathaur S., Yadav M., Gupta S., Anandharaman V., Reddy M.V. 2008. Filarial glutathione-S- transferase: a potential vaccine candidate against lymphatic filariasis. Vaccine, 26, 4094-4100. DOI: 10.1016/j.vaccine.2008.03.099CrossrefGoogle Scholar

  • Robinson M.W., Dalton J.P., O’Brien B.A., Donnelly S. 2013. Fasciola hepatica: The therapeutic potential of a worm secretome. International Journal for Parasitology, 43, 283-291. ttp://dx.doi.org/10.1016/j.ijpara.2012.11.004CrossrefGoogle Scholar

  • Saade F., Petrovsky N. 2012. Technologies for enhanced efficacy of DNA vaccines. Expert Review of Vaccines, 11, 189-209. DOI: 10.1586/erv.11.188PubMedCrossrefGoogle Scholar

  • Sato Y, Roman M., Tighe H., Lee D., Corr M., Nguyen M., Silverman G.J., Lotz M., Carson D.A., Raz E. 1996. Immunostimulatory DNA sequences necessary for effective intradermal gene immunization. Science, 273, 352-354Google Scholar

  • Shah M.A.A.,Yan R., Xu L., Song X., Li X. 2010. A recombinant DNA vaccine encoding Eimeria acervulina cSZ-2 induces immunity against experimental E. tenella infection. Veterinary Parasitology, 169, 185-189. DOI: 10.1016/j.vetpar.2009.12.035CrossrefGoogle Scholar

  • Shah M.A.A., Song X., Xu L.,Yan R., Li X. 2011. Construction of DNA vaccines encoding Eimeria acervulina cSZ-2 with chicken IL-2 and IFN-c and their efficacy against poultry coccidiosis. Research in Veterinary Science, 90, 72-77. DOI: 10.1016/j.rvsc.2010.05.003CrossrefGoogle Scholar

  • ShiW., Liu Q., Zhang J., Sun J., Jiang X., Geng J., Wang F., Xiao Y., Li H., Zhao X. 2014. Co-expression of EtMic2 protein and chicken interleukin-18 for DNA vaccine against chicken coccidiosis. Research in Veterinary Science, 97, 64-70. DOI: org/10.1016/j.rvsc.2014.05.001CrossrefGoogle Scholar

  • Smooker P.M., Kennedy N.J., Steeper K.R., Christopoulos H., Spithill T.W. 2001. Fasciola: Kinetics and quality of humoral responses to fatty acid binding protein and cathepsin l following delivery as DNA vaccines in mice. Experimental Parasitology, 97, 154-160, DOI: 10.1006/expr.2001.4601CrossrefGoogle Scholar

  • Song X., Xu L., Yan R., Huang X., Shah M.A.A., Li X. 2009. The optimal immunization procedure of DNA vaccine pcDNA- TA4-IL-2 of Eimeria tenella and its cross-immunity to Eimeria necatrix and Eimeria acervulina. Veterinary Parasitology 159, 30-36. DOI: 10.1016/j.vetpar.2008.10.015CrossrefGoogle Scholar

  • Song H., Yan R., Xu L., Song X., Shah M.A.A., Zhu H., Li X., 2010. Efficacy of DNA vaccines carrying Eimeria acervulina lactate dehydrogenase antigen gene against coccidiosis. Experimental Parasitology, 126, 224-231. DOI: 10.1016/j.exppara. 2010.05.015CrossrefGoogle Scholar

  • Song H., Qiu B.,Yan R Xu L., Song X., Li X. 2013. The protective efficacy of chimeric SO7/IL-2 DNA vaccine against coccidiosis in chickens Research in Veterinary Science, 94, 562-567. DOI: org/10.1016/j.rvsc.2012.11.006CrossrefGoogle Scholar

  • Schneider J., Langermans J.A., Gilbert S.C., Blanchard T.J., Twigg S., Naitza S., Hannan C.M., Aidoo M., Crisanti A., Robson K.J. et al. A prime-boost immunisation regimen using DNA followed by recombinant modified vaccinia virus Ankara induces strong cellular immune responses against the Plasmodium falciparum TRAP antigen in chimpanzees. Vaccine 2001, 19, 4595-4602PubMedCrossrefGoogle Scholar

  • Sun W., Song X., Yan R., Xu L., Li X. 2011.Vaccination of goats with a glutathione peroxidase DNA vaccine induced partial protection against Haemonchus contortus infection. Veterinary Parasitology 182, 239-247. DOI: 10.1016/j.vetpar.2011.05.024CrossrefGoogle Scholar

  • Thirugnanam S., Pandiaraja P., Ramaswamy K., Murugan V., Gnanasekar M., Nanadakumar K., Reddy M.V., Kaliraj P. 2007. Brugia malayi: comparison of protective immune responses induced by Bm-alt-2 DNA, recombinant Bm-ALT-2 protein and prime-boost vaccine regimens in a jird model. Experimental Parasitology, 116, 483-491. DOI: 10.1016/ j.exppara.2007.02.017CrossrefGoogle Scholar

  • Van Riet E., Hartgers F.C.,Yazdanbakhsh M. 2007. Chronic helminth infections induce immunomodulation: consequences and mechanisms. Immunobiology, 212, 6, 475-490. DOI: 10.1016/ j.imbio.2007.03.009CrossrefGoogle Scholar

  • Vaughan E.E., Dean D.A. 2006. Intracellular trafficking of plasmids during transfection is mediated by microtubules. Molecular Therapy, 13, 422-428. DOI: org/10.1016/j.ymthe.2005.10.004CrossrefGoogle Scholar

  • Waine G.J., Alarcon J.B., McManus D.P. 2002. Genetic immunization of mice with DNA encoding the 23 kDa transmembrane surface protein of Schistosoma japonicum (Sj23) induces antigen- specific immunoglobulin G antibodies. Parasite Immunology, 1999, 21, 377-381Google Scholar

  • Wang H., He S., Yao Y., Cong H., Zha H., Li T., Zhu X. 2009. Toxoplasma gondii: Protective effect of an intranasal SAG1 and MIC4 DNA vaccine in mice. Experimental Parasitology, 122, 226-232. DOI: 10.1016/j.exppara.2009.04.002CrossrefGoogle Scholar

  • Wahren W., Liu M.A. 2014. DNA vaccines: recent developments and the future. Vaccines, 2, 785-796. DOI: 10.3390/vaccines 2040785CrossrefGoogle Scholar

  • WesołowskaA., NorburyL., Januszkiewicz K., Jedlina L., Jaros S., Zawistowska- Deniziak A., ZygnerW., Wędrychowicz H. 2013. Evaluation of the immune response of male and female rats vaccinated with cDNA encoding a cysteine proteinase of Fasciola hepatica (FhPcW1). Acta Parasitologica, 58, 198-206. DOI :10.2478/s11686-013-0120-3CrossrefGoogle Scholar

  • Wedrychowicz H., Szymanski P., Panasiuk L.J., Bienkowska-Szewczyk K. 2002. Humoral immune response of rats vaccinated with cDNA or protein form of glutathione-S-transferase of Fasciola hepatica to infection with metacercariae of the fluke. Helminthologia, 39, 127-133Google Scholar

  • Wędrychowicz H., Lamparska M., Kęsik M., Kotomski G., Mieszczanek J., Jedlina-Panasiuk L., Płucienniczak A. 2003. The immune response of rats to vaccination with cDNA or protein forms of the cysteine proteinase of Fasciola hepatica. Veterinary Immunology and Immunopathology, 94, 83-93. DOI: 10.1016/S0165-2427(03)00085-0PubMedCrossrefGoogle Scholar

  • Williams J.A. 2013. Vector Design for Improved DNA Vaccine Efficacy, Safety and Production. Vaccines, 1, 225-249. DOI: 10.3390/vaccines1030225CrossrefGoogle Scholar

  • Wiśniewski M., Jaros S., Bąska P., Capello M., Wędrychowicz H. 2013. Ancylostoma ceylanicum metalloprotease 6 DNA vaccination induces partial protection against hookworm challenge infection. Acta Parasitologica 58, 376-383. DOI: 10.2478/s11686-013-0151-9CrossrefGoogle Scholar

  • Wolff J.A., Malone R.W., Williams P., Chong W., Acsadi G., Jani A., Felgner P.L. 1990. Direct gene transfer into mouse muscle in vivo. Science, 247, 1465-1468. DOI: org/10.1126/science. 1690918CrossrefGoogle Scholar

  • Xiang W., Qiong Z., Li-peng L., Kui T., Jian-wu G., Heng-ping S. 2009. The location of invasion- related protein MIC3 of Toxoplasma gondii and protective effect of its DNA vaccine in mice. Veterinary Parasitology, 166, 1-7. DOI: 10.1016/j.vetpar. 2009.08.014CrossrefGoogle Scholar

  • Xu Q., Song X., Xu L., Yan R., Shah M., Li X., 2008. Vaccination of chickens with a chimeric DNA vaccine encoding Eimeria tenella TA4 and chicken IL-2 induces protective immunity against coccidiosis. Veterinary Parasitology, 156, 319-323. DOI: 10.1016/j.vetpar.2008.05.025CrossrefGoogle Scholar

  • Xue M., He S., Zhang J., Cui Y., Yao Y.,Wang H. 2008a. Comparison of cholera toxin A2/B and murine interleukin-12 as adjuvants of Toxoplasma multi-antigenic SAG1-ROP2 DNA vaccine. Experimental Parasitology, 119, 352-357, DOI: 10.1016/ j.exppara.2008.03.005Google Scholar

  • Xue M., He S., Zhang J., Cui Y., Yao Y.,Wang H. 2008b. Evaluation of the immune response elicited by multi-antigenic DNA vaccine expressing SAG1, ROP2 and GRA2 against Toxoplasma gondii. Parasitology International 57,424-429. DOI: 10.1016/ j.parint.2008.05.001CrossrefGoogle Scholar

  • Yamakami K., Akao S., Sato M., Nitta Y., Miyazaki J., Tadakuma T. 2001. A single intradermal administration of soluble leishmanial antigen and plasmid expressing interleukin-12 protects BALB c mice from Leishmania major infection.. Parasitology International, 50, 81-91Google Scholar

  • Yan H.,Yuan Z., Petersen E., Zhang X., Zhou D., Liu Q., He Y., Lin R., Xu M., Chen X., Zhong X., Zhu X. 2011.Toxoplasma gondii: Protective immunity against experimental toxoplasmosis induced by a DNA vaccine encoding the perforin-like protein 1. Experimental Parasitology, 128, 38-43. DOI: 10.1016/j.exppara.2011.02.005CrossrefGoogle Scholar

  • Yan R., Sun W., Song X., Li X. 2013. Vaccination of goats with DNA vaccine encoding Dim-1 induced partial protection against Haemonchus contortus: A preliminary experimental study Research in Veterinary Science, 95, 189-199. DOI: org/10.1016/j.rvsc.2013.02.020CrossrefGoogle Scholar

  • You Z., Huang X., Hester J., Toh H.C., Chen S.Y. 2001. Targeting dendritic cells to enhance DNA vaccine potency. Cancer Research, 61, 3704-3711Google Scholar

  • Yu Q., Li J., Zhang X., Gong P., Zhang P., Li S., Wang H. 2010. Induction of immune responses in mice by a DNA vaccine encoding Cryptosporidium parvum Cp12 and Cp21 and its effect against homologous oocyst challenge. Veterinary Parasitology. 172, 1-7. DOI: 10.1016/j.vetpar.2010.04.036CrossrefGoogle Scholar

  • Yu L., Yamagishi J., Zhang S., Jin Ch., Aboge O.G., Zhang H., Zhang G., Tanaka T., Fujisaki K., Nishikawa Y., Xuan X. 2012. Protective effect of a prime-boost strategy with plasmid DNA followed by recombinant adenovirus expressing TgAMA1 as vaccines against Toxoplasma gondii infection in mice. Parasitology International, 61, 481-486. DOI: 10.1016/j.parint. 2012.04.001CrossrefGoogle Scholar

  • Yuan H.,You-en S., Long-jiang Y., Xiao-hua Z., Liu-zhe L.,Cash M., Lu Z., Zhi L., Deng-xin S. 2006. Studies on the protective immunity of Schistosoma japonicum bivalent DNA vaccine encoding Sj23 and Sj14. Experimental Parasitology, 115, 379-386. DOI: 10.1016/j.exppara.2006.09.022CrossrefGoogle Scholar

  • Zhao Y., Bao Y., Zhang L., Chang L., Jiang L., Liu Y., Zhang L., Qin J. 2013. Biosafety of the plasmid pcDNA3-1E of Eimeria acervulina in chicken. Experimental Parasitology, 133, 231-236, DOI: org/10.1016/j.exppara.2012.11.026CrossrefGoogle Scholar

  • Zhao G., Yan R., Muleke C.I., Sun Y., Xu L., Li X. 2012. Vaccination of goats with DNA vaccines encoding H11 and IL-2 induces partial protection against Haemonchus contortus infection. Veterinary Journal, 191, 94-100. DOI: 10.1016/j.tvjl.2010.12.023. Epub 2011 Feb 16.CrossrefGoogle Scholar

  • Zhu Y., Lu F., Dai Y., Wang X., Tang J., Zhao S., Zhang Ch., Zhang H., Lu S., Wang S. 2010. Synergistic enhancement of immunogenicity and protection in mice against Schistosoma japonicum with codon optimization and electroporation delivery of SjTPI DNA vaccines. Vaccine, 28, 5347-5355. DOI: 10.1016/j.vaccine.2010.05.017CrossrefGoogle Scholar

  • Zhu L., Liu H., Lu M., Long Q., Shi Y., Yu L. 2011.Construction, purification, and evaluation of multivalent DNA vaccine against Schistosoma japonicum Parasitology Research, 108, 115- 121. DOI: 10.1007/s00436-010-2040-6 CrossrefGoogle Scholar

About the article

Received: 2014-12-14

Revised: 2014-12-15

Accepted: 2014-12-17

Published Online: 2015-03-25

Published in Print: 2015-06-01

Citation Information: Acta Parasitologica, Volume 60, Issue 2, Pages 179–189, ISSN (Online) 1896-1851, ISSN (Print) 1230-2821, DOI: https://doi.org/10.1515/ap-2015-0026.

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